α1,3fucosyltransferase VI is expressed in HepG2 cells and codistributed with β1,4galactosyltransferase I in the Golgi apparatus and monensin-induced swollen vesicles

Glycobiology

Volumn 9, Issue 11, 1999, Pages 1273-1280

  Borsig, Lubor ^a,b   Imbach, Timo ^a   Höchli, Matthias ^a   Berger, Eric G ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fucosyltransferase; Golgi apparatus; Human liver; Monensin

Indexed keywords

ANTIBODY; FUCOSYLTRANSFERASE; GALACTOSYLTRANSFERASE; KIDNEY ENZYME; LIVER ENZYME; MONENSIN; BETA 1,4 GALACTOSYLTRANSFERASE I; BETA-1,4-GALACTOSYLTRANSFERASE I; GALACTOSIDE 3 FUCOSYLTRANSFERASE; GALACTOSIDE 3-FUCOSYLTRANSFERASE; HYBRID PROTEIN; TUMOR PROTEIN;

ARTICLE; CELL STRAIN HEPG2; CULTURE MEDIUM; ENZYME ASSAY; ENZYME BLOOD LEVEL; ENZYME LOCALIZATION; GOLGI COMPLEX; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MEMBRANE VESICLE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; BIOSYNTHESIS; CELL CULTURE; CHEMISTRY; CHO CELL; CONFOCAL MICROSCOPY; CRICETULUS; DRUG EFFECT; ENZYMOLOGY; EXTRACELLULAR SPACE; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GENETICS; HAMSTER; LIVER CELL CARCINOMA; LIVER TUMOR; METABOLISM; MOLECULAR WEIGHT; PATHOLOGY; SECRETION;

ANIMALS; CARCINOMA, HEPATOCELLULAR; CHO CELLS; CRICETINAE; CRICETULUS; EXTRACELLULAR SPACE; FUCOSYLTRANSFERASES; GALACTOSYLTRANSFERASES; GOLGI APPARATUS; HUMANS; LIVER NEOPLASMS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MOLECULAR WEIGHT; MONENSIN; NEOPLASM PROTEINS; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0032585916     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/9.11.1273     Document Type: Article

References (41)

1. Banting,G. and Ponnambalam,S. (1997) TGN38 and its orthologues - roles in post-TGN vesicle formation and maintenance of TGN morphology [review]. Biochim. Biophys. Acta Mol. Cell Res., 1355, 209-217.
2. Berger,E.G., Aegerter,E., Mandel,T. and Hauri,H.-P. (1986) Monoclonal antibodies to soluble, human milk galactosyltransferase (lactose synthase A protein) Carbohydr. Res., 149, 23-33.
3. Berger,E.G., Grimm,K., Bächi.T., Bosshart,H., Kleene,R. and Watzele,M. (1993) Double immunofluorescent staining of α2,6 sialyltransferase and β1,4 galactosyltransferase in monensin-treated cells: evidence for different Golgi compartments?. J. Cell. Biochem., 52, 275-88.
4. Borsig,L., Katopodis,A.G., Bowen,B.R. and Berger,E.G. (1998) Trafficking and localization studies of recombinant α-1,3-fucosyltransferase vi stably expressed in cho cells. Glycobiology, 8, 259-268.
5. Borsig,L., Kleene,R., Dinter,A. and Berger,E.G. (1996) Immunodetection of α1-3 fucosyltransferase (FucT-V). Eur. J. Cell Biol., 70, 42-53.
6. Brinkman-Van der Linden,E.C.M., Mollicone,R., Oriol,R., Larson,G., Van den Eijnden,D.H. and Van Dijk,W. (1996) A missense mutation in the FUT6 gene results in total absence of α 3-fucosylation of human α (1)-acid glycoprotein. J. Biol. Chem., 271, 14492-14495 (published erratum appeared in J. Biol. Chem., 271, 267-94, 1996).
7. x) antigen in the kidney but not on leucocytes. Immunogenetics, 5, 15-23.
8. Cameron,H.S., Szczepaniak,D. and Weston,B.W. (1995) Expression of human chromosome 19p α(1,3)-fucosyltransferase genes in normal tissues - alternative splicing, polyadenylation and isoforms. J. Biol. Chem., 270, 20112-20122.
9. De Vries,T., Palcic,M.P., Schoenmakers,P.S., Van Den Eijnden,D.H. and Joziasse,D.H. (1997) Acceptor specificity of GDP-Fuc:Gal β1→4GlcNAc-R α3-fucosyltransferase VI (FucT VI) expressed in insect cells as soluble, secreted enzyme. Glycobiology, 7, 921-927.
10. Dinter,A. and Berger,E.G. (1998) Golgi-disturbing agents [review]. Histochern. Cell Biol., 109, 571-590.
11. Gerber,A.C., Kozdrowski,I., Wyss, SR. and Berger,E.G. (1979) The charge heterogeneity of soluble human galactosyltransferases isolated from milk, amniotic fluid and malignant ascites. Eur. J. Biochem., 93, 453-460.
12. Goelz,S.E., Hession,C., Goff,D., Griffiths,B., Tizard,R., Newman,B., Chirosso,G. and Lobb,R. (1990) ELFT - a gene that directs the expression of an ELAM-1 ligand. Cell, 63, 1349-1356.
13. x motifs on complex-type N-glycans. J. Biol. Chem., 273, 30985-30994.
14. Homa,F.L., Hollander T., Lehman,D.J., Thomsen D.R.and Elhammer,A.P. (1993) Isolation and expression of a cDNA clone encoding a bovine UDPGalNAc:polypeptide N-acetylgalactosaminyltransferase. J. Biol. Chem., 268, 12609-12616.
15. Jezequel-Cuer,M., N'Guyen-Cong,H., Biou,D. and Durand,G. (1993) Oligosaccharide specificity of normal human hepatocyte α1-3 fucosyltransferase. Biochim. Biophys. Acta, 1157, 252-258.
16. Johnson,P.H., Donald,A.S., Clarke,J.L. and Watkins,W.M. (1995) Purification, properties and possible gene assignment of an α1,3-fucosyltransferase expressed in human liver. Glycoconj. J., 12, 879-893.
17. Kim,Y.S., Perdomo,J., Whitehead,J.S. and Curtis,K.J. (1972a) Glycosyltransferases in human blood. II. Study of serum galactosyltransferase and N-acetylgalactosaminyltransferase in patients with liver disease. J. Clin. Invest., 51, 2033-2039.
18. Kim,Y.S., Perdomo,J. and Whitehead,J.S. (1972b) Glycosyltransferases in human blood. I. Galactosyltransferase in human serum and erythrocyte membranes. J. Clin. Invest., 51, 2024-2032.
19. Koszdin,K.L. and Bowen,B.R. (1992) The cloning and expression of a human α-1,3 fucosyltransferase capable of forming the E-selectin ligand. Biochem. Biophys. Res. Commun., 187, 152-157.
20. Kukowska-Latallo,J.F., Larsen,D.R., Nair,R.P. and Lowe,J.B. (1990) A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the lewis blood group α (1,3/1,4) fucosyltransferase. Gene Dev., 4, 1288-1304.
21. Linstedt,A.D. and Hauri,H.P. (1993) Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. Mol. Biol. Cell. 4, 679-93.
22. Lowe,J.B. (1991) Molecular cloning, expression and uses of mammalian glycosyltransferases [review]. Semin. Cell Biol., 2, 289-307.
23. Lowe,J.B. (1997) Selectin ligands, leukocyte trafficking and fucosyltransferase genes. Kidney Int., 51, 1418-1426.
24. Macher,B.A., Holmes, EH., Swiedler S.J., Stults,C.L. and Srnka,C.A. (1991) Human α1-3 fucosyltransferases [review]. Glycobiology, 1, 577-584.
25. Masri,K.A., Appert,H.E. and Fukuda,M.N. (1988) Identification of the fulllength coding sequence for human galactosyltransferase (β-N-acetylglucosaminide: β1,4-galactosyltransferase). Biochem. Biophys. Res. Commun., 157, 657-663.
26. Mollenhauer,H.H., Morré,D.J. and Rowe,L.D. (1990) Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity [review]. Biochim. Biophys. Acta, 1031, 225-246.
27. Mollicone,R., Gibaud,A., François,A., Ratcliffe,M. and Oriol,R. (1990) Acceptor specificity and tissue distribution of three human α-3 fucosyltransferases. Eur. J. Biochem., 191, 169-176.
28. Mollicone,R., Candelier,J.J., Mennesson,B., Couillin,P., Venot,A.P. and Oriol,R. (1992) Five specificity patterns of (1-3)-α-L-fucosyltransferase activity defined by use of synthetic oligosaccharide acceptors. Differential expression of the enzymes during human embryonic development and in adult tissues. Carbohydr. Res., 228, 265-276.
29. Mollicone,R., Reguigne,I., Fletcher,A., Aziz,A., Rustam,M., Weston,B.W., Kelly,R.J., Lowe,J.B., Oriol,R. (1994) Molecular basis for plasma α (1,3)-fucosyltransferase gene deficiency (FUT6). J. Biol. Chem., 269, 12662-12671.
30. Natsuka,S., Gersten,K.M., Zenita,K., Kannagi,R. and Lowe,J.B. (1994) Molecular cloning of a cDNA encoding a novel human leukocyte α-1,3-fucosyltransferase capable of synthesizing the sialyl Lewis x determinant. J. Biol. Chem., 269, 16789-16794.
31. Palcic,M., Heerze,L., Srivastava,O. and Hindsgaul,O. (1989) A bisubstrate analog inhibitor for α (1-2)-fucosyltransferase. J. Biol. Chem., 264, 17174-17181.
32. Prescott,A.R., Lucocq,J.M., James,J., Lister,J.M. and Ponnambalam,S. (1997) Distinct compartmentalization of TGN46 and β-1,4-galactosyltransferase in Hela cells. Eur. J. Cell, Biol., 72, 238-246.
33. Roth,J. and Berger,E.G. (1982) Immunocytochemical localization of galactosyltransferase in HeLa cells: co-distribution with thiamine pyrophosphatase in trans-Golgi cisternae. J. Cell Biol., 93, 223-229.
34. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.
35. Sarnesto,A., Kohlin,T., Hindsgaul,O., Thurin,J. and Blaszczyk-Thurin,M. (1992) Purification of the secretor-type β-galactoside α1-2-fucosyltransferase from human serum. J. Biol. Chem., 267, 2737-2744.
36. Sasaki,K., Watanabe,E., Kawashima,K., Sekine,S., Dohi,T., Oshima,M., Hanai,N., Nishi,T. and Hasegawa,M. (1993) Expression cloning of a novel Gal β (1-3/1-4) GlcNAc α 2,3-sialyltransferase using lectin resistance selection. J. Biol. Chem., 268, 22782-22787.
37. Slot,J.W. and Geuze,H.J. (1983) Immunoelectron microscopic exploration of the Golgi complex. J. Histochem. Cytochem., 31, 1049-1056.
38. Strous,G.J.A.M. and Berger,E.G. (1982) Biosynthesis, intracellular transport and release of the Golgi enzyme galactosyltransferase (lactose synthetase A protein) in HeLa cells. J. Biol. Chem., 257, 7623-7628.
39. 2-terminal signal anchor. J. Biol. Chem., 262, 17735-17743.
40. Weston,B.W., Nair,R.P., Larsen,R.D. and Lowe,J.B. (1992a) Isolation of a novel human α (1,3)fucosyltransferase gene and molecular comparison to the human Lewis blood group α (1,3/1,4)fucosyltransferase gene. Syntenic, homologous, nonallelic genes encoding enzymes with distinct acceptor substrate specificities. J. Biol. Chem., 267, 4152-4160.
41. Weston,B.W., Smith,P.L., Kelly,R.J. and Lowe,J.B. (1992b) Molecular cloning of a fourth member of a human α (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x and difucosyl sialyl Lewis x epitopes [published erratum appeared in J. Biol. Chem., 1993, 268, 18398]. J. Biol. Chem., 267, 24575-24584.