IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Lum, Mabel ^a   Morona, Renato ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; HYBRID PROTEIN; ICSA AUTOTRANSPORTER; UNCLASSIFIED DRUG; AMPICILLIN; ANTIINFECTIVE AGENT; BETA LACTAMASE; BETA LACTAMASE TEM 1; BETA-LACTAMASE TEM-1; DNA BINDING PROTEIN; MALTOSE BINDING PROTEIN; TRANSCRIPTION FACTOR; VIRG PROTEIN, SHIGELLA FLEXNERI;

ALPHA CHAIN; ARTICLE; BETA CHAIN; CELL SURFACE; COMPARATIVE STUDY; ESCHERICHIA COLI K 12; IMMUNOFLUORESCENCE MICROSCOPY; NONHUMAN; OUTER MEMBRANE; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; SHIGELLA FLEXNERI; BIOSYNTHESIS; CELL MEMBRANE; DRUG EFFECT; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; METABOLISM;

ESCHERICHIA COLI K12; SHIGELLA FLEXNERI;

AMPICILLIN; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAMASES; CELL MEMBRANE; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; MALTOSE-BINDING PROTEINS; MICROSCOPY, FLUORESCENCE; RECOMBINANT FUSION PROTEINS; SHIGELLA FLEXNERI; TRANSCRIPTION FACTORS;

EID: 84856584036     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-20     Document Type: Article

References (34)

1. van Bloois E, Winter RT, Kolmar H, Fraaije MW. Decorating microbes: surface display of proteins on Escherichia coli. Trends Biotechnol 2011, 29:79-86. 10.1016/j.tibtech.2010.11.003, 21146237.
2. Henderson IR, Navarro-Garcia F, Desvaux M, Fernandez RC, Ala'Aldeen D. Type V protein secretion pathway: the Autotransporter story. Microbiol Mol Biol Rev 2004, 68:692-744. 10.1128/MMBR.68.4.692-744.2004, 539010, 15590781.
3. Jose J, Bernhardt R, Hannemann F. Functional display of active bovine adrenodoxin on the surface of E. coli by Chemical Incorporation of the [2Fe-2S] Cluster. Chem Bio Chem 2001, 2:695-701.
4. Jose J, Betscheider D, Zangen D. Bacterial surface display library screening by target enzyme labeling: Identification of new human cathepsin G inhibitors. Anal Biochem 2005, 346:258-267. 10.1016/j.ab.2005.08.019, 16225837.
5. Maurer J, Jose J, Meyer T. Autodisplay: one-component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli. J Bacteriol 1997, 179:794-804. 178762, 9006035.
6. Kjærgaard K, Hasman H, Schembri MA, Klemm P. Antigen 43-mediated Autotransporter display, a versatile bacterial cell surface presentation system. J Bacteriol 2002, 184:4197-4204. 10.1128/JB.184.15.4197-4204.2002, 135209, 12107137.
7. Klauser T, Pohlner J, Meyer TF. Selective extracellular release of cholera toxin B subunit by Escherichia coli: dissection of Neisseria Iga beta-mediated outer membrane transport. EMBO J 1992, 11:2327-2335. 556700, 1600950.
8. Kramer U, Rozos K, Apfel H, Autenrieth IB, Lattemann CT. Autodisplay: development of an efficacious system for surface display of antigenic determinants in Salmonella vaccine strains. Infect Immun 2003, 71:1944-1952. 10.1128/IAI.71.4.1944-1952.2003, 152032, 12654812.
9. Jose J, Kramer J, Klauser T, Pohlner J, Meyer TF. Absence of periplasmic DsbA oxidoreductase facilitates export of cysteine-containing passenger proteins to the Escherichia coli cell surface via the Iga beta autotransporter pathway. Gene 1996, 178:107-110. 10.1016/0378-1119(96)00343-5, 8921899.
10. Kadokura H, Beckwith J. Four cysteines of the membrane protein DsbB act in concert to oxidize its substrate DsbA. EMBO J 2002, 21:2354-2363. 10.1093/emboj/21.10.2354, 126001, 12006488.
11. Veiga E, De Lorenzo V, Fernandez LA. Structural tolerance of bacterial autotransporters for folded passenger protein domains. Mol Microbiol 2004, 52:1069-1080. 10.1111/j.1365-2958.2004.04014.x, 15130125.
12. Lett MC, Sasakawa C, Okada N, Sakai T, Makino S, Yamada M, Komatsu K, Yoshikawa M. virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence. J Bacteriol 1989, 171:353-359. 209595, 2644195.
13. Makino S, Sasakawa C, Kumata K, Kurata T, Yishikawa M. A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in S. flexneri 2a. Cell 1986, 46:551-555. 10.1016/0092-8674(86)90880-9, 3524856.
14. Goldberg MB. Actin-based Motility of Intracellular Microbial Pathogens. Microbiol Mol Biol Rev 2001, 65:595-626. 10.1128/MMBR.65.4.595-626.2001, 99042, 11729265.
15. Suzuki T, Lett MC, Sasakawa C. Extracellular Transport of VirG Protein in Shigella. J Biol Chem 1995, 270:30874-30880. 10.1074/jbc.270.52.30874, 8537341.
16. Stevens JM, Galyov EE, Stevens MP. Actin-dependent movement of bacterial pathogens. Nat Rev Microbiol 2006, 4:91-101. 10.1038/nrmicro1320, 16415925.
17. Suzuki T, Lett MC, Sasakawa C. Extracellular transport of VirG Protein in Shigella. J Biol Chem 1995, 270:30874-30880. 10.1074/jbc.270.52.30874, 8537341.
18. Brandon LD, Goehring N, Janakiraman A, Yan AW, Wu T, Beckwith J, Goldberg MB. IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributed. Mol Microbiol 2003, 50:45-60. 10.1046/j.1365-2958.2003.03674.x, 14507362.
19. Charles M, Pérez M, Konil JH, Goldberg MB. Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc Natl Acad Sci USA 2001, 98:9871-9876. 10.1073/pnas.171310498, 55545, 11481451.
20. Brandon LD, Goldberg MB. Periplasmic Transit and Disulfide Bond Formation of the Autotransporter Shigella Protein IcsA. J Bacteriol 2001, 183:951-958. 10.1128/JB.183.3.951-958.2001, 94963, 11208794.
21. Oomen CJ, van Ulsen P, van Gelder P, Feijen M, Tommassen J, Gros P. Structure of the translocator domain of a bacterial autotransporter. EMBO J 2004, 23:1257-1266. 10.1038/sj.emboj.7600148, 381419, 15014442.
22. Barnard TJ, Dauntin N, Lukacik P, Bernstein HD, Buchanan SK. Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Nat Struct Mol Biol 2007, 4:1214-1220.
23. van den Berg B. Crystal Structure of a Full-Length Autotransporter. J Mol Biol 2010, 396:627-633. 10.1016/j.jmb.2009.12.061, 20060837.
24. Egile C, d'Hauteville H, Parsot C, Sansonetti PJ. SopA, the outer membrane protease responsible for polar localization of IcsA in Shigella flexneri. Mol Microbiol 1997, 23:1063-1073. 10.1046/j.1365-2958.1997.2871652.x, 9076742.
25. Shere KD, Sallustio S, Manessis A, D'Aversa TG, Goldberg MB. Disruption of IcsP, the major Shigella protease that cleaves IcsA, accelerates actin-based motility. Mol Microbiol 1997, 25:451-462. 10.1046/j.1365-2958.1997.4681827.x, 9302008.
26. Fukuda I, Suzuki T, Munakata H, Hayashi N, Katayama E, Yoshikawa M, Sasakawa C. Cleavage of Shigella surface protein VirG occurs at a specific site, but the secretion is not essential for intracellular spreading. J Bacteriol 1995, 177:1719-1726. 176798, 7896693.
27. Saurí A, Oreshkova N, Soprova Z, Jong WS, Sani M, Peters PJ, Luirink J, van Ulsen P. Autotransporter β-Domains have a specific function in protein secretion beyond outer-membrane targeting. J Mol Biol 2011, 412:553-567. 10.1016/j.jmb.2011.07.035, 21806993.
28. Dautin N, Bernstein HD. Residues in a Conserved α-Helical Segment are required for cleavage but not secretion of an Escherichia coli serine protease autotransporter passenger domain. J Bacteriol 2011, 193:3748-3756. 10.1128/JB.05070-11, 3147522, 21642456.
29. May KL, Morona R. Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. J Bacterio 2008, 190:4666-4676.
30. Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 1995, 177:4121-4130. 177145, 7608087.
31. Lattemann CT, Maurer J, Gerland E, Meyer TF. Autodisplay: functional display of active beta-lactamase on the surface of Escherichia coli by the AIDA-I autotransporter. J Bacteriol 2000, 182:3726-3733. 10.1128/JB.182.13.3726-3733.2000, 94543, 10850987.
32. Rutherford N, Charbonneau ME, Berthiaume F, Betton JM, Mourez M. The periplasmic folding of a cysteineless autotransporter passenger domain interferes with its outer membrane translocation. J Bacteriol 2006, 188:4111-4116. 10.1128/JB.01949-05, 1482886, 16707702.
33. Morona R, Van Den Bosch L. Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri. FEMS Microbiol Lett 2003, 221:173-180. 10.1016/S0378-1097(03)00210-6, 12725923.
34. Van Den Bosch L, Manning PA, Morona R. Regulation of O-antigen chain length is required for Shigella flexneri virulence. Mol Microbiol 1997, 23:765-775. 10.1046/j.1365-2958.1997.2541625.x, 9157247.