SopA, the outer membrane protease responsible for polar localization of IcsA in Shigella flexneri

Molecular Microbiology

Volumn 23, Issue 5, 1997, Pages 1063-1073

  Egile, Coumaran ^a   D'Hauteville, Hélène ^a   Parsot, Claude ^a   Sansonetti, Philippe J ^a  

^a INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

F ACTIN; OUTER MEMBRANE PROTEIN; PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GROWTH; CONTROLLED STUDY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SHIGELLA FLEXNERI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SHIGELLA FLEXNERI;

EID: 0031025789     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.2871652.x     Document Type: Article

References (45)

1. Adam, T., Arpin, M., Prévost, M.-C., Gounon, P., and Sansonetti, P.J. (1995) Cytoskeletal rearrangements and the functional role of T-plastin during entry of Shigella flexneri into HeLa cells. J Cell Biol 129: 367-381.
2. Allaoui, A., Mounier, J., Prévost, M.-C., Sansonetti, P.J., and Parsot, C. (1992) icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread. Mol Microbiol 6: 1605-1616.
3. Benjelloun-Touimi, Z., Sansonetti, P.J., and Parsot, C. (1995) SepA, the major extracellular protein of Shigella flexneri: autonomous secretion and involvement in tissue invasion. Mol Microbiol 17: 123-135.
4. Bernardini, M.L., Mounier, J., d'Hauteville, H., Coquis-Rondon, M., and Sansonetti, P.J. (1989) Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci USA 86: 3867-3871.
5. Clerc, P., and Sansonetti, P.J. (1987) Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerisation and myosin accumulation. Infect Immun 55: 2681-2688.
6. Fukuda, I., Suzuki, T., Munakata, H., Hayashi, N., Katayama, E., Yoshikawa, M., and Sasakawa, C. (1995) Cleavage of Shigella surface protein VirG occurs at a specific site, but the secretion is not essential for intracellular spreading. J Bacteriol 177: 1719-1726.
7. Goldberg, M.B., and Theriot, A.J. (1995) Shigella flexneri surface protein IcsA is sufficient to direct actin-based motility. Proc Natl Acad Sci USA 92: 6572-6576.
8. Goldberg, M.B., Barzu, O., Parsot, C., and Sansonetti, P.J. (1993) Unipolar localisation and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement. Infect Immun 175: 2189-2196.
9. Goldberg, M.B., Theriot, J.A., and Sansonetti, P.J. (1994) Regulation of surface presentation of IcsA, a Shigella protein essential to intracellular movement and spread, is growth phase dependent. Infect Immun 62: 5664-5668.
10. Grodberg, J., and Dunn, J.J. (1988) OmpT encodes the Escherichia coli membrane protease that cleaves T7 RNA polymerase during purification. J Bacteriol 170: 1245-1253.
11. Grodberg, J., and Dunn, J.J. (1989) Comparison of Escherichia coli K-12 outer membrane protease OmpT and Salmonella typhimurium E protein. J Bacteriol 171: 2903-2905.
12. d'Hauteville, H., and Sansonetti, P.J. (1992) Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intercellular spread of Shigella flexneri. Mol Microbiol 6: 833-841.
13. d'Hauteville, H., Dufourcq-Lagelouse, R., Nato, F., and Sansonetti, P.J. (1996) Lack of cleavage of IcsA in Shigella flexneri causes aberrant movement and allows demonstration of a cross-reactive eukaryotic protein. Infect Immun 64: 511-517.
14. Jenal, U., and Shapiro, L. (1996) Cell cycle-controlled proteolysis of a flagellar motor protein that is asymmetrically distributed in the Caulobacter predivisional cell. EMBO J 15: 2393-2406.
15. Kaufmann, A., Stierhof, Y.-D., and Henning, U. (1994) New outer membrane associated protease of Escherichia coli K-12. J Bacteriol 176: 359-367.
16. Klauser, T., Pohlner, P., and Meyer, T. (1993) The secretion pathway of IgA protease-type proteins in Gram negative bacteria. Bioassays 15: 799-805.
17. Kocks, C., Hellio, R., Gounon, P., Ohayon, H., and Cossart, P. (1993) Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly. J Cell Sci 105: 699-710.
18. Kocks, C., Marchand, J.B., Gouin, E., d'Hauteville, H., Sansonetti, P.J., Carlier, M.-F., and Cossart, P. (1995) The unrelated proteins ActA of Listeria monocytogenes and IcsA of Shigella flexneri are sufficient to confer actin-based motility on Listeria innocua and Escherichia coli, respectively. Mol Microbiol 18: 413-423.
19. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
20. Lett, M.C., Sasakawa, C., Okada, N., Sakai, T., Makino, S., Yamada, M., Komatsu, K., and Yoshikawa, M. (1988) virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the VirG protein and determination of the complete coding sequence. J Bacteriol 171: 353-359.
21. Maddock, J.R., Alley, M.R.K., and Shapiro, L. (1993) Polarized cells, polar actions. J Bacteriol 175: 7125-7129.
22. Makino, S., Sasakawa, C., Kamata, K., Kurata, T., and Yoshikawa, M.A. (1986) A genetic determinant required for continuous reinfection of adjacent cells on large plasmid of Shigella flexneri. Cell 46: 551-555.
23. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
24. Maurelli, A.T., Baudry, B., d'Hauteville, H., Hale, T.L., and Sansonetti, P.J. (1985) Cloning of plasmid DNA sequences involved in invasion of HeLa cells in Shigella flexneri. Infect Immun 49: 164-171.
25. Ménard, R., Sansonetti, P.J., and Parsot, C. (1994) The secretion of the Shigella flexneri lpa invasins is induced by the epithelial cell and controlled by lpaB and lpaD. EMBO J 13: 5293-5302.
26. Miller, V.L., and Mekalanos, J.J. (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170: 2575-2583.
27. Nakata, N., Tobe, T., Fukuda, I., Suzuki, T., Komatsu, K., Yoshikawa, M., and Sasakawa, C. (1993) The absence of a surface protease OmpT determines the intercellular spreading ability of Shigella: the relationship between the ompT and kcpA loci. Mol Microbiol 9: 459-468.
28. Oaks, E.V., Wingfield, M.E., and Formal, S.B. (1985) Plaque formation by virulent Shigella flexneri. Infect Immun 48: 124-129.
29. Parsot, C., and Sansonetti, P.J. (1995) Invasion and pathogenesis of Shigella infections. Curr Top Microbiol Immunol 209: 25-42.
30. Rajakumar, K., Jost, B.H., Sasakawa, C., Okada, N., Yoshikawa, M., and Adler, B. (1994) Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence. J Bacteriol 176: 2362-2373.
31. Sandlin, R.C., Lampel, K.A., Keasler, S.P., Goldberg, M.B., Stolzer, A.L., and Maurelli, A.T. (1995) Avirulence of rough mutants of Shigella flexneri: requirement of O antigen for correct unipolar localisation of lcsA in the bacterial outer membrane. Infect Immun 63: 229-237.
32. Sansonetti, P.J., Kopecko, D.J., and Formal, S.B. (1982) Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun 35: 852-860.
33. Sansonetti, P.J., Ryter, A., Clerc, P., Maurelli, A.T., and Mounier, J. (1986) Multiplication of Shigella flexneri within HeLa cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. Infect Immun 51: 461-469.
34. Sansonetti, P.J., Arondel, J., Fontaine, A., d'Hauteville, H., and Bernardini, M.L. (1991) ompB (osmo-regulation) and icsA (cell to cell spread) mutants of Shigella flexneri: vaccine candidates and probes to study the pathogenesis of shigellosis. Vaccine 9: 416-422.
35. Sansonetti, P.J., Mounier, J., Prévost, M.C., and Mège, R.M. (1994) Cadherin expression is required for the spread of Shigella flexneri between epithelial cells. Cell 76: 829-839.
36. Sansonetti, P.J., Arondel, J., Cavaillon, J.-M., and Huerre, M. (1995) Role of lnterleukin-1 in the pathogenesis of experimental shigellosis. J Clin Invest 96: 884-892.
37. Sen, K., and Nikaido, H. (1991) Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin. J Bacteriol 173: 926-928.
38. Shapiro, L. (1993) Protein localisation and asymmetry in the bacterial cell. Cell 73: 841-855.
39. Smith, G.A., Portnoy, D.A., and Theriot, J.A. (1995) Asymmetric distribution of the Listeria monocytogenes ActA protein is required and sufficient to direct actin-based motility. Mol Microbiol 17: 945-951.
40. Smith, G.A., Theriot, J.A., and Portnoy, D.A. (1996) The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin. J Cell Biol 135: 647-660.
41. Sodeinde, O.A., and Goguen, J.D. (1989) Nucleotide sequence of the plasminogen activator gene of Yersinia pestis: relationship to ompT of Escherichia coli and gene E of Salmonella typhimurium. Infect Immun 57: 1517-1523.
42. Sugimura, K., and Nishihara, T. (1988) Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and ompT. J Bacteriol 170: 5625-5632.
43. Suzuki, T., Lett, M.C., and Sasakawa, C. (1995) Extracellular tansport of VirG protein in Shigella. J Biol Chem 270: 30874-80.
44. Theriot, J.A. (1995) The cell biology of infection by intracellular bacterial pathogens. Annu Rev Cell Dev Biol 11:213-239.
45. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.