Fiber-optic calcium monitoring of dendritic activity in vivo

Cold Spring Harbor Protocols

Volumn 7, Issue 2, 2012, Pages 218-225

  Murayama, Masanori ^a   Larkum, Matthew ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; REAGENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOLOGICAL MONITORING; CALCIUM SIGNALING; CELL FUNCTION; DENDRITE; EPIFLUORESCENCE MICROSCOPY; EQUIPMENT; FIBER OPTICS; NEOCORTEX; NONHUMAN; PYRAMIDAL NERVE CELL; RAT; ANIMAL; BEHAVIOR; CHEMISTRY; METHODOLOGY; PHYSIOLOGY;

ANIMALIA;

ANIMALS; BEHAVIOR; CALCIUM; DENDRITES; FIBER OPTIC TECHNOLOGY; RATS;

EID: 84856481949     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot067835     Document Type: Article

References (17)

1. Adelsberger H, Garaschuk O, Konnerth A. 2005. Cortical calcium waves in resting newborn mice. Nat Neurosci 8: 988-990.
2. Chia TH, Levene MJ. 2009. Microprisms for in vivo multi-layer cortical imaging. J Neurophysiol 102: 1310-1314.
3. Engelbrecht CJ, Johnston RS, Seibel EJ, Helmchen F. 2008. Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo. Opt Express 16: 5556-5564.
4. Flusberg BA, Nimmerjahn A, Cocker ED, Mukamel EA, Barretto RP, Ko TH, Burns LD, Jung JC, Schnitzer MJ. 2008. High-speed, miniaturized fluorescence microscopy in freely moving mice. Nat Methods 5: 935-938.
5. Göbel W, Helmchen F. 2007. New angles on neuronal dendrites in vivo. J Neurophysiol 98: 3770-3779.
6. Helmchen F, Svoboda K, Denk W, Tank DW. 1999. In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons. Nat Neurosci 2: 989-996.
7. Kerr JN, Greenberg D, Helmchen F. 2005. Imaging input and output of neocortical networks in vivo. Proc Natl Acad Sci 102: 14063-14068.
8. Kitamura K, Judkewitz B, Kano M, Denk W, Häusser M. 2008. Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo. Nat Methods 5: 61-67.
9. Larkum ME, Nevian T, Sandler M, Polsky A, Schiller J. 2009. Synaptic integration in tuft dendrites of layer 5 pyramidal neurons: A new unifying principle. Science 325: 756-760.
10. Murayama M, Larkum ME. 2009a. Enhanced dendritic activity in awake rats. Proc Natl Acad Sci 106: 20482-20486.
11. Murayama M, Larkum ME. 2009b. In vivo dendritic calcium imaging with a fiberoptic periscope system. Nat Protoc 4: 1551-1559.
12. Murayama M, Pérez-Garci E, Lüscher HR, Larkum ME. 2007. Fiberoptic system for recording dendritic calcium signals in layer 5 neocortical pyramidal cells in freely moving rats. J Neurophysiol 98: 1791-1805.
13. Murayama M, Pérez-Garci E, Nevian T, Bock T, Senn W, Larkum ME. 2009. Dendritic encoding of sensory stimuli controlled by deep cortical interneurons. Nature 457: 1137-1141.
14. 2+ imaging of local neuronal circuits. Neuron 53: 789-803.
15. Sawinski J, Wallace DJ, Greenberg DS, Grossmann S, Denk W, Kerr JND. 2009. Visually evoked activity in cortical cells imaged in freely moving animals. Proc Natl Acad Sci 106: 19557-19562.
16. Svoboda K, Denk W, Kleinfeld D, Tank DW. 1997. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385: 161-165.
17. 2+ influx into dendritic tufts of layer 2/3 neocortical pyramidal neurons in vitro and in vivo. J Neurosci 23: 8558-8567.