Cell preparation

Flow Cytometry: Principles and Applications

Volumn , Issue , 2007, Pages 17-58

  McCarthy, Desmond A ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Antibody antigen interaction; immunolabeling; permeabilization; preparation procedure

Indexed keywords

EID: 84856270662     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-1-59745-451-3_2     Document Type: Chapter

References (77)

1. Reilly, J. T. and Barnett, D. (2001) UK NEQAS for leucocyte immunophenotyping: The first 10 years. J. Clin. Pathol. 54, 508-511.
2. Shankey, T. V., Rabinovich, P. S., Bagwell, B., et al. (1993) Guidelines for implementation of clinical DNA cytometry. Cytometry 14, 472-477.
3. Ormerod, M. G., Tribukait, B., and Giaretti, W. (1998) Concensus report of the task force on standardisation of DNA flow cytometry in clinical pathology. DNA Flow Cytometry Task Force of the European Society for Analytical Cellular Pathology. Anal. Cell. Pathol. 17, 103-110.
4. Robinson, J. P. (ed.) The Purdue Cytometry CD-ROM Vol. 3. 1997 (Parker, J. and Stewart, C., guest eds.). Purdue University, West Lafayette, IN.
5. Robinson, J. P. (ed.) The Purdue Cytometry CD-ROM Vol. 4. 1998 (Watson, J., guest ed.). Purdue University, West Lafayette, IN.
6. Bazil, V. (1995) Physiological enzymatic cleavage of leukocyte membrane molecules. Immunology Today 16, 135-140.
7. Hamblin, A., Taylor, M., Bernhagen, J., et al. (1992) A method of preparing blood leukocytes for flow cytometry which prevents up-regulation of leukocyte integrins. J. Immunol. Methods 146, 219-228.
8. Hagberg, I. A. and Lyberg, T. (2000) Blood platelet activation evaluated by flow cytometry: optimised methods for clinical studies. Platelets 11, 137-150.
9. +T cell counts by flow cytometry. II. The use of fixed whole blood in resource-poor settings. J. Immunol. Methods 257, 145-154.
10. McCarthy, D. A., Macey, M. G., Cahill, M. R., and Newland, A. C. (1994) Effects of fixation on quantification of the expression of leukocyte function-associated surface antigens. Cytometry 17, 39-49.
11. Canonico, B., Zamai, L., Burattini, S., et al. (2004) Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy. J. Immunol. Methods 295, 67-78.
12. Cahill, M. R., Macey, M. G., and Newland, A. C. (1993) Fixation with formaldehyde induces expression of activation dependent platelet membrane glycoproteins, P selectin (CD62) and GP53 (CD63). Br. J. Haematol. 84, 527-529.
13. Schmidt, V., Hilberg, T., Franke, G., Glaser, D., and Gabriel, H. H. (2003) Paraformaldehyde fixation induces a systematic activation of platelets. Platelets 14, 287-294.
14. Leino, L. and Sorvajarvi, K. (1992) CD11b is a calcium-dependent epitope in human neutrophils. Biochem. Biophys. Res. Commun. 87, 195-200.
15. McCarthy, D. A. and Macey, M. G. (1993) A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J. Immunol. Methods 163, 155-160.
16. Mody, M., Lazarus, A. H., Semple, J. W., and Freedman, J. (1999) Preanalytical requirements for flow cytometric evaluation of platelet activation: choice of anticoagulant. Transfus. Med. 9, 147-154.
17. Macey, M. G., McCarthy, D. A., Azam, U., Milne, T., Golledge, P., and Newland, A.C. (2003) Ethylenediaminetetraacetic acid plus citrate-theophylline-adenosinedipyridamole (EDTA-CTAD): A novel anticoagulant for the flow cytometric assessment of platelet and neutrophil activation ex vivo in whole blood. Cytometry 52B, 30-40.
18. Bergereon, M., Nicholson, J. K., Phaneuf, S., et al. (2002) Selection of lymphocyte gating protocol has an impact on the level of reliability of T-cell subsets in aging specimens. Cytometry 50, 53-61.
19. Ashmore, L. M., Shopp, G. M., and Edwards, B. E. (1989) Lymphocyte subset analysis by flow cytometry. Comparison of three different staining techniques and effects of blood storage. J. Immunol. Methods 118, 209-215.
20. Hensleigh, P. A., Waters, V. B., and Herzenberg, L. A. (1993) Human T lymphocyte differentiation antigens: effects of blood sample storage on LEU antibody binding. Cytometry 3, 453-455.
21. Repo, H., Jansson, S-E., and Leirisalo-Repo, M. (1993) Flow cytometric determination of CD11b upregulation in vivo. J. Immunol. Methods 164, 193-202.
22. Romeu, M. A., Mestre, M., Gonzalez, L., et al. (1992) Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll®-Paque separation and cryopreservation. J. Immunol. Methods 154, 7-10.
23. Macey, M. G., McCarthy, D. A., and Newland, A. C. (1997) The Q-Prep system: effects on the apparent expression of leuocyte cell surface antigens. Cytometry 30, 67-71.
24. Macey, M. G., McCarthy, D. A., Milne, T., Cavenagh, J. D., and Newland, A. C. (1999) Comparative study of five commercial reagents for preparing normal and leukaemic lymphocytes for immunophenotypic analysis by flow cytometry. Cytometry 38, 153-160.
25. McCarthy, D. A. and Macey, M. G. (1996) Novel anticoagulants for flow cytometric analysis of live leukocytes in whole blood. Cytometry 23, 196-204.
26. Smith, P. J., Wiltshire, M., Davies, S., Patterson, L. H., and Hoy, T. (1999) A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J. Immunol. Methods 229, 131-139.
27. Terstappen, L. W. M. M., Shah, V. O., Conrad, M. P., Recktenwald, D., and Loken, M. R., (1988) Discriminating between intact and damaged cells in fixed flow cytometric samples. Cytometry 9, 477-484.
28. Terstappen, L. W. M. M., Johnson, D., Mickaels, R. A., et al. (1991) Multidimensional flow cytometric blood cell differentiation without erythrocyte lysis. Blood Cells 17, 585-602.
29. Himmelfarb, J., Hakim, R. M., Holbrook, D. G., Leeber, D. A., and Ault, K. A. (1992) Detection of granulocyte reactive oxygen species formation in whole blood using flow cytometry. Cytometry 13, 83-89.
30. Macey, M. G., McCarthy, D. A., Howells, G. L., Curtis, M. A., King, G., and Newland, A.C. (1998) Multiparametric flow cytometric analysis of polymorphonuclear leuocytes in whole blood from patients with adult rapidly progressive periodontitis reveals low expression of the adhesion molecule L-selectin (CD62L). Cytometry 34, 152-158.
31. Fearon, D. T. and Collins, L. A. (1983) Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J. Immunol. 130, 370-375.
32. Forsyth, K. D. and Levinsky, R. J. (1990) Preparative procedures of cooling and re-warming increases leukocyte integrin expression and function on neutrophils. J. Immunol. Methods 128, 159-163.
33. Haslett, C., Guthrie, L. A., Kopaniak, M. M., Johnston, R. B., and Henson, P. M. (1985) Modulation of neutrophil function by preparative methods or trace concentrations of bacterial lipopolysaccharide. Am. J. Pathol. 119, 101-110.
34. Macey, M. G., McCarthy, D. A., Vordermeier, S., Newland, A. C., and Brown, K. A. (1995) Effects of cell purification methods on CD11b and L-selectin expression as well as the adherence and activation of leucocytes. J. Immunol. Methods 181, 211-219.
35. Recktenwald, D. and Radbruch, A. (eds.) (1997) Cell Separation Methods and Applications. Marcel Dekker, New York.
36. Radbruch, A. and Recktenwald, D. (1995) Detection and isolation of rare cells. Curr. Opin. Immunol. 7, 270-273.
37. Bagamery, K., Kvell, K., Barnet, M., Landau, R., and Graham, J. (2005) Are platelets activated after a rapid, one-step density gradient centrifugation? Evidence from flow cytometric analysis. Clin. Lab. Haematol. 27, 75-77.
38. Macey, M. G., Jiang, X. P., Vey, P., McCarthy, D., and Newland, A. C. (1992) Expression of functional antigens on neutrophils: effects of preparation. J. Immunol. Methods 149, 37-42.
39. Tamul, K. R., Schmitz, J. L., Kane, K., and Folds, J. D. (1995) Comparison of the effects of Ficoll®-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies. Clin. Diagn. Lab. Immunol. 2, 337-342.
40. Rickwood, D. (1983) Iodinated Density Gradient Media: A Practical Approach. IRL Press, Oxford, UK.
41. Boyum, A., Lovhaug, D., Tresland, L., and Nordlie, E. M. (1991) Separation of leucocytes: improved cell purity by fine adjustments of gradient medium density and osmolality. Scand. J. Immunol. 34, 697-712.
42. McCarthy, D. A., Perry, J. D., Holburn, C. M., et al. (1984) Centrifugation of normal and rheumatoid atrhritis blood on Ficoll®-Hypaque and Ficoll®-Nycodenz solutions. J. Immunol. Methods 73, 415-425.
43. Bignold, L. P. and Ferrante, A. (1987) Mechanism of separation of polymorphonuclear leukocytes from whole blood by the one-step Hypaque-Ficoll® method. J. Immunol. Methods 96, 29-33.
44. Ferrante, A. and Thong, Y. H. (1980) Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leukocytes from human blood by the Hypaque-Ficoll® method. J. Immunol. Methods 36, 109-117.
45. Needham, P. (1987) Separation of human blood using 'Mono-Poly Resolving Medium'. J. Immunol. Methods 99, 283-284.
46. McFaul, S. J. (1990) A method for isolating neutrophils from moderate volumes of human blood. J. Immunol. Methods 130, 5-18.
47. Pertoft, H., Hirtenstein, M., and Kagedal, L. (1979) Cell separations in a new density medium, Percoll®, in Cell Populations, Methodological Surveys (B) Biochemistry, Vol. 9 (Reid, E., ed.), Ellis Horwood, Chichester, UK, pp. 67-80.
48. Denholm, E.M. and Wolber, F.M. (1991) A simple method for the purification of human peripheral blood monocytes: A substitute for Sepracell-MN. J. Immunol. Methods 144, 247-251.
49. Vettese-Dadey, M. (1999) Going their separate ways. A profile of products for cell separation. Scientist 13, 18-27.
50. Vuorte, J., Jansson, S. E., and Repo, H. (2001) Evaluation of red blood cell lysing solutions in the study of neutrophil oxidative burst by the DCFH assay. Cytometry 43, 290-296.
51. Bossuyt, X., Marti, G. E., and Fleisher, T. A. (1997) Comparative analysis of whole blood lysis methods for flow cytometry. Cytometry 30, 124-133.
52. Visscher, D. W. and Crissman, H. A. (1994) Dissociation of intact cells from tumors and normal tissues, in Methods in Cell Biology, Vol. 41, Part A, 2nd edit. (Darzynkiewicz Z., Robinson J.P., Crissman, H.A., eds.), Academic Press, New York, pp. 1-13.
53. French, D. and Edsall, J. T. (1945) The reactions of formaldehyde with amino acids and proteins, in Advances in Protein Chemistry, Vol. 2 (Anderson, M.L. and Edsall, J.T., eds.), Academic Press, New York, pp. 277-335.
54. Kappelmayer, J., Gratam, J. W., Karaszi, E., et al. (2000) Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protcols. J. Immunol. Methods 24, 53-65.
55. Krutzik, P. O. and Nolan, G. P. (2003) Intracellular phosho-protein staining techniques for flow cytometry. Monitoring single cell signaling events. Cytometry 55A, 61-70.
56. Bauer, K. D. and Jacobberger, J. W. (1984) Analysis of intracellular proteins. Methods Cell Biol. 41, 352-373.
57. Darzynkiewicz, Z., Gong, J., Juan, G., Ardelt, B., and Traganos, F. (1996) Cytometry of cyclin proteins. Cytometry 25, 1-13.
58. Francis, C. and Conolly, M.C. (1996) Rapid single step method for flow cytometric detection of surface and intracellular antigens using whole blood. Cytometry 25, 58-70.
59. Koester, S. K. and Bolton, W. E. (2000) Intracellular markers. J. Immunol. Methods 243, 99-106.
60. Karlsson, R. and Roos, H. (1997) Reaction kinetics, in Principles and Practice of Immunoassay, 2nd edit. (Price, C.P. and Newman, D.J., eds.), Stockton Press, New York, pp. 101-122.
61. Sklar, L. A. and Finney, D. A. (1982) Analysis of ligand-receptor interactions with the fluorescence activated cell sorter. Cytometry 3, 161-165.
62. Pallis, M. and Robins, R. A. (1995) What you need to know when you go with the flow: pitfalls in the use of flow cytometry. Ann. Rheum. Dis. 54, 785-786.
63. Roe, R., Robins, R. A., Laxton, R. R., and Baldwin, R. W. (1985) Kinetics of divalent monoclonal antibody binding to tumour cell surface antigens using flow cytometry - standardization and mathematical analysis. Mol. Immunol. 22, 11-21.
64. Davis, K. A., Abrams, B., Iyer, S. B., Hoffman, R. A., and Bishop, J. E. (1998) Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation. Cytometry 33, 197-205.
65. Junghans, R. P. (1999) Cruel antibody intentions: Cellular antigen enumeration by 'saturation' binding. Immunology Today 20, 401-406.
66. Robins, R. A. (1998) Flow cytometric analysis of cell surface antigen density, in Methods in Molecular Biology: Immunochemical Protocols (Pound, J.D., ed.), Humana Press, Totowa, NJ, pp. 319-336.
67. Robins, R. A. (2000) Counting antigens using antibodies. Immunology Today 21, 154-155.
68. Gratama, J. W., D'hautcourt, J. L., Mandy, F., et al. (1998) Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis. Cytometry 33, 166-178.
69. Barnett, D., Storie, I., Wilson, G. A., Granger, V., and Reilly, J. T. (1998) Determination of antibody-binding capacity (ABC): The need for standardization. Clin. Lab. Haematol. 20, 155-164.
70. Cohen, J. H. M., Aubry, J. P., Jouvin, M. H., et al. (1987) Enumeration of CR1 complement receptors on erythrocytes using a new method for detecting low density cell surface antigens by flow cytometry. J. Immunol. Methods 99, 53-61.
71. Schlenke, P., Frohn, C., Kluter, H., et al. (1998) Evaluation of a flow cytometric method for simultaneous leukocyte phenotyping and quantification by fluorescent microspheres. Cytometry 33, 310-317.
72. Nicholson, J. K., Stein, D., Mui, T., Mack, R., Hubbard, M., and Denny, T. (1997) Evaluation of a method of counting absolute numbers of cells with a flow cytometer. Clinical, Diagnostic and Laboratory Immunology 4, 309-313.
73. +T-lymphocytes. Cytometry 57, 47-52.
74. Schmid, I., Merlin, S., and Perfetto, S. P. (2003) Biosafety concerns for shared flow cytometry core facilities. Cytometry 56, 113-119.
75. Schmid, I., Roederer, M., Koup, R. A., Ambrozak, D., and Perfetto, S. P. (2004) Biohazard sorting. Methods Cell Biol. 75, 221-240.
76. Owens, M. A. and Loken, M. R. (1995) Flow Cytometry Principles for Clinical Laboratory Practice: Quality Assurance for Quantitative Immunophenotyping. Wiley-Liss, New York.
77. Valenstein, P. N. and Collinge, M. L. (1997) Laboratory safety, in Manual of Clinical Laboratory Immunology, 5th edit. (Rose, N. R., de Marcio, E. C., Folds, J. D., Lane, H. C., and Nakamura, R. M., eds.), ASM Press, Washington, DC, pp. 1213-1222.