Evaluation of a method for counting absolute numbers of cells with a flow cytometer

Clinical and Diagnostic Laboratory Immunology

Volumn 4, Issue 3, 1997, Pages 309-313

  Nicholson, Janet K A ^a,c   Stein, Dana ^b   Mui, Tammy ^b   Mack, Richard ^b   Hubbard, Marjorie ^a   Denny, Thomas ^b  

^a NATL CENTER FOR INFECTIOUS DISEASES   (United States)

^b RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

^c CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD16 ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD56 ANTIGEN; CD8 ANTIGEN;

ARTICLE; CELL COUNT; FLOW CYTOMETRY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; LABORATORY TEST; LYMPHOCYTE SUBPOPULATION; MAJOR CLINICAL STUDY; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; VIRUS INFECTION;

EID: 0030983427     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.4.3.309-313.1997     Document Type: Article

References (12)

1. Bentley, S. A., A. Johnson, and C. A. Bishop. 1993. A parallel evaluation of four automated hematology analyzers. Am. J. Clin. Pathol. 100:626-632.
2. + T-cell determinations in persons with human immunodeficiency virus infection. Morbid. Mortal. Weekly Rep. 41(RR-8):1-17.
3. Denny, T. N., B. D. Jensen, E. I. Gavin, A. G. Louzao, F. A. Vella, J. M. Oleske, and W. Wong. 1995. Determination of CD4 and CDS lymphocyte subsets by a new alternative fluorescence immunoassay. Clin. Diagn. Lab. Immunol. 2:330-336.
4. DeWolf, F., M. Roos, J. M. A. Lange, J. T. M. Houweling, R. A. Coutinho, J. Van der Noordaa, P. T. Schellekens, and J. Goudsmit. 1988. Decline in CD4+ cell numbers reflects increase in HIV-1 replication. AIDS Res. Hum. Retroviruses 4:433-440.
5. Johnson, D., D. Hirschkorn, and M. P. Busch. 1995. Evaluation of four alternative methodologies for determination of absolute CD4+ lymphocyte counts. The National Heart, Lung, and Blood Institute retrovirus epidemiology donor study. J. Acquired Immune Defic. Syndr. Hum. Retrovirol. 10:522-530.
6. McDougal, J. S., J. K. A. Nicholson, and A. Mawle. 1987. Effects of HIV infection on the immune system, p. 51-88. In R. Madhok, C. D. Forbes, and B. L. Evatt (ed.), Blood, blood products and AIDS. Chapman and Hall, Ltd., London, England.
7. Mercolino, T. J., M. C. Connelly, E. J. Meyer, M. D. Knight, J. W. Parker, G. T. Stelzer, and G. DeChirico. 1995. Immunologic differentiation of absolute lymphocyte count with an integrated flow cytometric system: a new concept for absolute T cell subset determinations. Cytometry 22:49-59.
8. Nicholson, J. K. A., W. M. Velleca, S. Jubert, T. A. Green, and L. Bryan. 1994. Evaluation of alternative CD4 technologies for the enumeration of CD4 lymphocytes. J. Immunol. Methods 177:43-54.
9. Paxton, H., M. Pins, G. Denton, A. D. McGonigle, P. S. Meisner, and J. P. Phair. 1995. Comparison of CD4 cell count by a simple enzyme-linked immunosorbent assay using the TRAx CD4 test kit and by flow cytometry and hematology. Clin. Diagn. Lab. Immunol. 2:104-114.
10. Rosenberg, Z. F., and A. S. Fauci. 1991. Immunopathogenic mechanisms of human immunodeficiency virus (HIV) infection, p. 141-161. In R. C. Gallo and G. Jay (ed.), The human retroviruses. Academic Press, Inc., San Diego, Calif.
11. Schenker, E. L., L. E. Hultin, K. D. Bauer, J. Ferbas, J. B. Margolick, and J. V. Giorgi. 1993. Evaluation of a dual-color flow cytometry immunophenotyping panel in a multicenter quality assurance program. Cytometry 14: 307-317.
12. Turner, B. J., F. M. Hecht, and R. B. Ismail. 1994. CD4+ T-lymphocyte measures in the treatment of individuals infected with human immunodeficiency virus type I. Arch. Intern. Med. 154:1561-1572.