Methylene as a possible universal footprinting reagent that will include hydrophobic surface areas: Overview and feasibility: Properties of diazirine as a precursor

Protein Science

Volumn 9, Issue 12, 2000, Pages 2506-2517

  Richards, Frederic M ^a   Lamed, Raphael ^a,b   Wynn, Richard ^a,c   Patel, Darshan ^a,d   Olack, Gerard ^a  

^a YALE UNIVERSITY   (United States)

^b TEL AVIV UNIVERSITY   (Israel)

^c DUPONT COMPANY   (United States)

^d Box 576 ^*  (United States)

Author keywords

Diazirine; Methylene; Nonpolar; Photochemical labeling; Protein footprinting; Surface area

Indexed keywords

CARBENE; OLIGOMER; SULFADIAZINE;

ARTICLE; BINDING SITE; CHEMICAL LABELING; COVALENT BOND; HYDROPHOBICITY; MASS SPECTROMETRY; PHOTOLYSIS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN INTERACTION; PROTEIN STRUCTURE;

EID: 0034493026     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.12.2506     Document Type: Article

References (31)

1. DNA strand breaking by the hydroxyl radical is governed by the accessible surface area of the hydrogen atoms of the DNA backbone
2. Mass spectrometry of peptides and proteins
3. Differential labeling of the catalytic subunit of cAMP-dependent protein kinase with acetic anhydride: Substrate-induced conformational changes
4. Protein ladder sequencing
5. Probing the conformation of proteins with diazirine, a minimal photochemical reagent suitable for labeling cavities. On protein condensation: In honor of Gregorio Weber
6. Conformation dependent labeling of proteins with diazirine, a generally reactive photoprobe
7. Principles of stable isotope distribution
8. Indiscriminate reaction of methylene with the carbon-hydrogen bond
9. Mapping staphylococcal nuclease conformation using an EDTA-Fe derivative attached to genetically engineered cysteine residues
10. The chemical modification of proteins bY group-specific and site-specific reagents
11. Hydroxyl radical-induced hydrogen/deuterium exchange in amino acid carbon-hydrogen bonds
12. Identification of the sites of hydroxyl radical reaction with peptides by hydrogen/deuterium exchange: Prevalence of reactions with the side chains
13. A direct method of preparation of diazirine
14. Protein footprinting by the combined us of reversible and irreversible lysine modification
15. Myoglobin and the structure of proteins
16. A three-dimensional model of the myoglobin molecule obtained by X-ray analysis
17. Primary photochemical processes. Part II. The absorption spectrum and photochemical decomposition of diazomethane
18. The interpretation of protein structures: Estimation of static accessibility
19. Notiz uber eine einfache Synthese des Cyclodiazomethans
20. Seeing the surface: Analysis of covalent modification of proteins using diazirine
21. Oxidative polypeptide cleavage mediated by EDTA-Fe covalently linked to cysteine residues
22. Protein folding kinetics by combined use of rapid mixing techniques and NMR observation of individual amide protons
23. Hyroxyl radical footprinting: A high-resolution method for mapping protein-DNA contacts
24. Reactivity and intersystem crossing of singlet methylene in solution