Evaluation of normalization methods on GeLC-MS/MS label-free spectral counting data to correct for variation during proteomic workflows

Journal of the American Society for Mass Spectrometry

Volumn 22, Issue 12, 2011, Pages 2199-2208

  Gokce, Emine ^a   Shuford, Christopher M ^a   Franck, William L ^a   Dean, Ralph A ^a   Muddiman, David C ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Fold change; GeLC MS MS; Label free; Mass spectrometry; NanoLC MS; Normalized spectral abundance factor; Orbitrap XL; Proteomics; Quantification; Significance test; Spectral counting; Total spectral count normalization

Indexed keywords

FOLD-CHANGE; GELC-MS/MS; LABEL FREE; NANO-LC-MS; NORMALIZED SPECTRAL ABUNDANCE FACTOR; ORBITRAP; PROTEOMICS; QUANTIFICATION; SIGNIFICANCE TEST; SPECTRAL COUNTING; TOTAL SPECTRAL COUNT NORMALIZATION;

GELS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY;

PROTEINS;

FUNGAL PROTEIN; MYOGLOBIN; OVALBUMIN;

ACCURACY; ARTICLE; CONCENTRATION (PARAMETERS); CONIDIUM; CONTROLLED STUDY; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; GEL CHROMATOGRAPHY; MAGNAPORTHE; MAGNAPORTHE ORYZAE; NONHUMAN; PREDICTION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEOMICS; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; RICE BLAST; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY;

ANIMALS; CHICKENS; CHROMATOGRAPHY, GEL; FUNGAL PROTEINS; HORSES; MYOGLOBIN; OVALBUMIN; PEPTIDE MAPPING; PROTEOMICS; REGRESSION ANALYSIS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EQUIDAE; MAGNAPORTHE GRISEA; MAGNAPORTHE ORYZAE;

EID: 84856162864     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0237-2     Document Type: Article

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