Labeling Schwann cells with CFSE - An in vitro and in vivo study

Journal of Neuroscience Methods

Volumn 125, Issue 1-2, 2003, Pages 83-91

  Li, Xiuming ^a   Dancausse, Hector ^a   Grijalva, Israel ^a   Oliveira, Maria ^a   Levi, Allan D O ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

CFSE; Fluorescence; Labeling; Nude rats; Schwann cells; Spinal cord; Transplantation

Indexed keywords

5 CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; 6 CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; BIOLOGICAL MARKER; ESTER DERIVATIVE; FLUORESCENT DYE; TRACER; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CELL CULTURE; EUTHANASIA; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GRAY MATTER; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; INCUBATION TIME; INJECTION; LIPOPHILICITY; LUMBAR VERTEBRA; MIXED CELL CULTURE; MOLECULE; MORPHOLOGICAL TRAIT; NONHUMAN; PRIORITY JOURNAL; RAT; SCHWANN CELL; SPINAL CORD; SURVIVAL RATE; TISSUE GRAFT; VERTEBRA; WHITE MATTER;

EID: 0038577628     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(03)00044-X     Document Type: Article

References (33)

1. Ansselin A.D., Fink T., Davey D.F. Peripheral nerve regeneration through nerve guides seeded with adult Schwann cells. Neuropathol. Appl. Neurobiol. 23:1997;387-398.
2. Berry M., Rees L., Hall S., Yiu P., Sivers J. Optic axons regenerate into sciatic isografts only in the presence of Schwann cells. Brain Res. Bull. 20:1988;223-231.
3. Butcher E.C., Scollay R.G., Wetissman I.L. Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. II. Potential application to studies of lymphocyte migration and maturation. J. Immunol. Methods. 37:1980;109-121.
4. Casella G.T., Bunge R.P., Wood P.M. Improved method for harvesting human Schwann cells from mature peripheral nerve and expansion in vitro. Glia. 1996;327-338.
5. Evercooren A.B.V., Gransmuller A., Clerin E., Gumpel M. Hoechst 33342 a suitable fluorescent marker for Schwann cells after transplantation in mouse spinal cord. Neurosci. Lett. 131:1991;241-244.
6. Feltri M.L., Scherer S.S., Wrabetz L., Kamholz J., Shy M.E. Mitogen-expanded Schwann cells retain the capacity to myelinate regenerating axons after transplantation into rat sciatic nerve. Proc. Natl. Acad. Sci. USA. 89:1992;8827-8831.
7. Felts P.A., Smith K.J. Conduction properties of central nerve fibers remyelinated by Schwann cells. Brain Res. 574:1992;178-192.
8. Fujioka H., Hunt P.J., Rozga J., Wu G.D., Cramer V., Demetriou A.A., et al. Carboxyfluorescein (CFSE) labeling of hepatocytes for short-term localization following intraportal transplantation. Cell Transplant. 3:1994;397-408.
9. Groszer M., Erickson R., Scripture-Adams D.D., Lesche R., Trumpp A., Zack J.A., et al. Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivo. Science. 194:2001;2186-2189.
10. Gruber H.E., Leslie K.P., Ingram J.A., Hanley E.N. Jr. Optimization of 5-(and 6-)carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro. Biotech. Histochem. 75:2000;118-123.
11. Hasbold J., Gett A.V., Rush J.S., Deenick A., Avery D., Jun H., Hodgkin P.D. Quantitative analysis of lymphocyte differentiation and proliferation in vitro using carboxyfluorescein diacetate succinimidyl ester. Immunol. Cell Biol. 77:1999;516-522.
12. Hermanns S., Wunderlich G., Rosenbaum C., Hanemann C.O., Muller H.W., Stichel C.C. Lack of immune responses to immediate or delayed implanted allogeneic and xenogeneic Schwann cell suspension. Glia. 21:1997;299-314.
13. Honig MG, Hume RI. Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J Cell Biol 1986;103:171-87.
14. Horan P.K., Slezak S.E. Stable cell membrane labeling. Nature. 340:1989;167-168.
15. Horan P.K., Melnicoff M.J., Jensen B.D., Slezak S.E. Fluorescent cell labeling of lymphocytes for studies of cell tracking. Methods Cell. Biol. 33:1990;469-490.
16. Karrer FM, Reitz BL, Hao L, Lafferty KJ. Fluorescein labeling of murine hepatocytes for identification after intrahepatic transplantation. Transplant Proc 1992;24:2820-1.
17. Levi A.D.O., Bunge R.P. Studies of myelin formation after transplantation of human Schwann cells into the severe combined immunodeficient mouse. Exp. Neurol. 130:1994;41-52.
18. Levi A.D.O. Charaterization of the technique involved in isolating Schwann cells from adult human peripheral nerve. J. Neurosci. Methods. 68:1996;21-26.
19. Lyons A.B. Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester. Immunol. Cell Biol. 77:(6):1999;509-515.
20. Lyons A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J. Immunol. Methods. 243:2000;147-154.
21. Mosahebi A., Woodward B., Green C., Martin R., Terenghi G. Long-term effect of vital labeling on mixed Schwann cell cultures. Histochem. J. 32:2000;337-343.
22. Mosahebi A., Woodward B., Wiberg M., Martin R., Terenghi G. Retroviral labeling of Schwann cells: in vitro characterization and in vivo transplantation to improve peripheral nerve regeneration. Glia. 34:2001;8-17.
23. Olszewski W.L. In vivo migration of immune cells. 1987;CRC Press, Boca Raton, Florida.
24. Ostrowska A., Karrer K.M., Bilir B.M. Histological identification of purified and cryopreserved allogeneic hepatocytes following transplantation in a murine model without host immunosuppression. Transplant. Int. 12:1999;188-194.
25. Paramore C.G., Turner D.A., Madison R.D. Fluorescent labeling of dissociated fetal cells for tissue culture. J. Neurosci. Methods. 44:1992;7-17.
26. Plant G.W., Ramon-Cueto A., Bunge M.B. Transplantation of Schwann cells and ensheathing glia to improve regeneration in adult spinal cord. Ingoglia N.A., Murray M. Axonal regeneration in the central nervous system. 2001;529-561 Marcel Dekker, New York.
27. Pleasure D., Kreider B., Sobue G., Ross A.H., Koprowski H., Sounedfeld K.H., et al. Schwann-like cells cultured from human dermal neurofibromas. Ann. N.Y. Acad. Sci. 486:1986;227-240.
28. Steedman H.F. Polyester wax: a new ribboning embedding medium for histology. Nature. 179:1957;1345.
29. Weijer K., Uittenbogaart C.H., Voordouw A., Couwenberg F., Seppen J., Bianca B., et al. Intrathymic and extrathymic development of human plasmacytoid dendritic cell precursors in vivo. Blood. 99:2002;2752-2759.
30. Weston S.A., Parish C.R. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J. Immunol. Methods. 133:1990;87-96.
31. Xu X.M., Guenard V., Kleitman N., Aebischer P., Bunge M.B. A combination of BDNF and NT-3 promotes supraspinal axonal regeneration into Schwann cell grafts in adult rat thoracic spinal cord. Exp. Neurol. 134:1995;161-172.
32. Xu X.M., Guenard V., Kleitman N., Bunge M.B. Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. J. Comp. Neurol. 351:1995;145-160.
33. Xu X.M., Chen A., Guenard V., Kleitman N., Bunge M.B. Bridging Schwann cell transplants promotes axonal regeneration from both the rostral and caudal stumps of transected adult rat spinal cord. J. Neurocytol. 26:1997;1-16.