Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering)

Methods in Enzymology

Volumn 504, Issue , 2012, Pages 221-235

  Owen, Dylan M ^a   Williamson, David ^a   Magenau, Astrid ^a   Gaus, Katharina ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Immune synapse; LAT; PALM; Super resolution

Indexed keywords

GREEN FLUORESCENT PROTEIN; LAT PROTEIN;

ARTICLE; CELL MEMBRANE; MATHEMATICAL ANALYSIS; MICROSCOPY; PALMITOYLATION; PHOTOACTIVATED LOCALIZATION MICROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS;

EID: 84855965214     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-391857-4.00011-2     Document Type: Chapter

References (25)

1. E. Betzig, G.H. Patterson, R. Sougrat, O.W. Lindwasser, S. Olenych, J.S. Bonifacino, M.W. Davidson, J. Lippincott-Schwartz, and H.F. Hess Imaging intracellular fluorescent proteins at nanometer resolution Science 313 2006 1642 1645 (Pubitemid 44414039)
2. W.H. Binder, V. Barragan, and F.M. Menger Domains and rafts in lipid membranes Angew. Chem. Int. Ed. 42 2003 5802 5827 (Pubitemid 38029589)
3. D.A. Brown Lipid rafts, detergent-resistant membranes, and raft targeting signals Physiology 21 2006 430 439 (Pubitemid 44879252)
4. A. Getis, and J. Franklin Second-order neigborhood analysis of mapped point patterns Ecology 68 1987 473 477
5. T.J. Gould, V.V. Verkhusha, and S.T. Hess Imaging biological structures with fluorescence photoactivation localization microscopy Nat. Protoc. 4 2009 291 308
6. M.G.L. Gustafsson Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy J. Microsc. 198 2000 82 87 (Pubitemid 30316807)
7. L.C. Hartgroves, J. Lin, H. Langen, T. Zech, A. Weiss, and T. Harder Synergistic assembly of linker for activation of T cells signaling protein complexes in T cell plasma membrane domains J. Biol. Chem. 278 2003 20389 20394 (Pubitemid 36799240)
8. M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, and M. Sauer Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes Angew. Chem. Int. Ed. 47 2008 6172 6176
9. B. Hein, K.I. Willig, and S.W. Hell Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell Proc. Natl. Acad. Sci. USA 105 2008 14271 14276
10. S.W. Hell, and J. Wichmann Breaking the diffraction resolution limit by stimulated emission: Stimulated-emission-depletion fluorescence microscopy Opt. Lett. 19 1994 780 782
11. R. Henriques, M. Lelek, E.F. Fornasiero, F. Valtorta, C. Zimmer, and M.M. Mhlanga QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ Nat. Methods 7 2010 339 340
12. S.T. Hess, T.P.K. Girirajan, and M.D. Mason Ultra-high resolution imaging by fluorescence photoactivation localization microscopy Biophys. J. 91 2006 4258 4272 (Pubitemid 44887284)
13. B.F. Lillemeier, M.A. Mortelmaier, M.B. Forstner, J.B. Huppa, J.T. Groves, and M.M. Davis TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation Nat. Immunol. 11 2010 90 96
14. D.M. Owen, S. Oddos, Sunil Kumar, D.M. Davis, M.A. Neil, P.M. French, M.L. Dustin, A.I. Magee, and M. Cebecauer High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells Mol. Membr. Biol. 27 2010 178 189
15. D.M. Owen, C. Rentero, J. Rossy, A. Magenau, D. Williamson, M. Rodriguez, and K. Gaus PALM imaging and cluster analysis of protein heterogeneity at the cell surface J. Biophoton. 3 2010 446 454
16. G.L.W. Perry SpPack: Spatial point pattern analysis in Excel using Visual Basic for applications (VBA) Environ. Modell. Softw. 19 2004 559 569 (Pubitemid 38355855)
17. B.D. Ripley Modelling spatial patterns J. R. Stat. Soc. Ser. B Stat. Methodol. 39 1977 172 192
18. M.J. Rust, M. Bates, and X. Zhuang Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) Nat. Methods 3 2006 793 796
19. H. Shroff, C.G. Galbraith, J.A. Galbraith, and E. Betzig Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics Nat. Methods 5 2008 417 423 (Pubitemid 351619100)
20. K. Simons, and E. Ikonen Functional rafts in cell membranes Nature 387 1997 569 572 (Pubitemid 27248754)
21. N. Tanimura, S.I. Saitoh, S. Kawano, A. Kosugi, and K. Miyake Palmitoylation of LAT contributes to its subcellular localization and stability Biochem. Biophys. Res. Commun. 341 2006 1177 1183 (Pubitemid 43221789)
22. R.E. Thompson, D.R. Larson, and W.W. Webb Precise nanometer localization analysis for individual fluorescent probes Biophys. J. 82 2002 2775 2783 (Pubitemid 34441314)
23. J. Wiedenmann, S. Ivanchenko, F. Oswald, F. Schmitt, C. Röcker, A. Salih, K.-D. Spindler, and G.U. Nienhaus EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion Proc. Natl. Acad. Sci. USA 101 2004 15905 15910 (Pubitemid 39507900)
24. D.J. Williamson, D.M. Owen, J. Rossy, A. Magenau, M. Wehrmann, J.J. Gooding, and K. Gaus Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events Nat. Immunol. 12 2011 655 662
25. W. Zhang, R.P. Trible, and L.E. Samelson LAT palmitoylation: Its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation Immunity 9 1998 239 246 (Pubitemid 28400037)