Generation of primary astrocyte cultures devoid of contaminating microglia

Methods in Molecular Biology

Volumn 814, Issue , 2012, Pages 61-79

  Uliasz, Tracy F ^a   Hamby, Mary E ^a   Jackman, Nicole A ^a   Hewett, James A ^b   Hewett, Sandra J ^b  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

^b SYRACUSE UNIVERSITY   (United States)

Author keywords

Astrocytes; Cell culture; Lysosomotropism; Microglia; Mitotic inhibition

Indexed keywords

COLONY STIMULATING FACTOR RECEPTOR; CYTARABINE; DRUG DERIVATIVE; LEUCINE; LEUCINE METHYL ESTER;

ARTICLE; ASTROCYTE; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; METABOLISM; METHODOLOGY; MICROGLIA;

ASTROCYTES; CELL CULTURE TECHNIQUES; CYTARABINE; LEUCINE; MICROGLIA; RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR;

EID: 84855938740     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-452-0_5     Document Type: Article

References (26)

1. Montgomery, D. L. (1994) Astrocytes: form, functions, and roles in disease, Vet Pathol 31, 145-167.
2. Parri, R., and Crunelli, V. (2003) An astrocyte bridge from synapse to blood fl ow, Nat Neurosci 6, 5-6.
3. Fields, R. D., and Stevens-Graham, B. (2002) New insights into neuron-glia communication, Science 298, 556-562.
4. Janzer, R. C. (1993) The blood-brain barrier: cellular basis, J Inherit Metab Dis 16, 639-647. (Pubitemid 23237046)
5. Doetsch, F. (2003) The glial identity of neural stem cells, Nat Neurosci 6, 1127-1134. (Pubitemid 37346029)
6. Araque, A., Carmignoto, G., and Haydon, P. G. (2001) Dynamic signaling between astrocytes and neurons, Annu Rev Physiol 63, 795-813. (Pubitemid 32304337)
7. Andersson, M., and Hanse, E. (2010) Astrocytes impose postburst depression of release probability at hippocampal glutamate synapses, J Neurosci 30, 5776-5780.
8. Hertz, L., and Zielke, H. R. (2004) Astrocytic control of glutamatergic activity: astrocytes as stars of the show, Trends in Neurosciences 27, 735.
9. Tommaso, F. (2009) Communication between neurons and astrocytes: relevance to the modulation of synaptic and network activity, Journal of Neurochemistry 108, 533-544.
10. Hao, C., Guilbert, L. J., and Fedoroff, S. (1990) Production of colony-stimulating factor- 1 (CSF-1) by mouse astroglia in vitro, J Neurosci Res 27, 314-323.
11. Giulian, D., and Ingeman, J. E. (1988) Colonystimulating factors as promoters of ameboid microglia, J Neurosci 8, 4707-4717. (Pubitemid 19015153)
12. Thery, C., Stanley, E. R., and Mallat, M. (1992) Interleukin 1 and tumor necrosis factor-alpha stimulate the production of colony-stimulating factor 1 by murine astrocytes, J Neurochem 59, 1183-1186.
13. Hewett, S. J. (1999) Interferon-gamma reduces cyclooxygenase-2-mediated prostaglandin E2 production from primary mouse astrocytes independent of nitric oxide formation, J Neuroimmunol 94, 134-143. (Pubitemid 29071253)
14. Uliasz, T. F., and Hewett, S. J. (2000) A microtiter trypan blue absorbance assay for the quantitative determination of excitotoxic neuronal injury in cell culture, J Neurosci Methods 100, 157-163.
15. Tedeschi, B., Barrett, J. N., and Keane, R. W. (1986) Astrocytes produce interferon that enhances the expression of H-2 antigens on a subpopulation of brain cells, J Cell Biol 102, 2244-2253. (Pubitemid 16052164)
16. Solenov, E., Watanabe, H., Manley, G. T., and Verkman, A. S. (2004) Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-defi cient mice, measured by a fl uorescence quenching method, Am J Physiol Cell Physiol 286, C426-432. (Pubitemid 38146367)
17. Swanson, R. A., Liu, J., Miller, J. W., Rothstein, J. D., Farrell, K., Stein, B. A., and Longuemare, M. C. (1997) Neuronal regulation of glutamate transporter subtype expression in astrocytes, J Neurosci 17, 932-940. (Pubitemid 27043952)
18. Thiele, D. L., Kurosaka, M., and Lipsky, P. E. (1983) Phenotype of the accessory cell necessary for mitogen-stimulated T and B cell responses in human peripheral blood: delineation by its sensitivity to the lysosomotropic agent, L-leucine methyl ester, J Immunol 131, 2282-2290. (Pubitemid 14227424)
19. Guillemin, G., Boussin, F. D., Croitoru, J., Franck-Duchenne, M., Le Grand, R., Lazarini, F., and Dormont, D. (1997) Obtention and characterization of primary astrocyte and microglial cultures from adult monkey brains, J Neurosci Res 49, 576-591. (Pubitemid 27381245)
20. Giulian, D., Vaca, K., and Corpuz, M. (1993) Brain glia release factors with opposing actions upon neuronal survival, J Neurosci 13, 29-37. (Pubitemid 23028725)
21. Simmons, M. L., and Murphy, S. (1992) Induction of nitric oxide synthase in glial cells, J Neurochem 59, 897-905.
22. Bowman, C. C., Rasley, A., Tranguch, S. L., and Marriott, I. (2003) Cultured astrocytes express toll-like receptors for bacterial products, Glia 43, 281-291. (Pubitemid 37047684)
23. Giulian, D., and Baker, T. J. (1986) Characterization of ameboid microglia isolated from developing mammalian brain, J Neurosci 6, 2163-2178. (Pubitemid 16043659)
24. Hamby, M. E., Uliasz, T. F., Hewett, S. J., and Hewett, J. A. (2006) Characterization of an improved procedure for the removal of microglia from confl uent monolayers of primary astrocytes, J Neurosci Methods 150, 128-137. (Pubitemid 41770807)
25. Rose, K., Goldberg, M.P., Choi, D.W. (1993) Methods In Toxicology Volume 1A, Vol. 1A, Chapter 4: Cytotoxicity in Murine Neocortical Cell Culture; Academic Press; pp 46-60.
26. Krady, J. K., Basu, A., Levison, S. W., and Milner, R. J. (2002) Differential expression of protein tyrosine kinase genes during microglial activation, Glia 40, 11-24. (Pubitemid 35181340)