Quantifying plasmid copy number to investigate plasmid dosage effects associated with directed protein evolution

Methods in Molecular Biology

Volumn 834, Issue , 2012, Pages 33-48

  Million Weaver, Samuel ^a   Alexander, David L ^a   Allen, Jennifer M ^a   Camps, Manel ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

ALKBH2; ColE1 plasmid; Directed evolution; Green fluorescent protein; Methyl methane sulfonate; Mutagenesis; N methyl N nitro N nitrosoguanidine; Plasmid copy number; R loop; Recombinant gene expression; RNA I; RNA II; Transformation

Indexed keywords

PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIUM TRANSFORMATION; DIRECTED MOLECULAR EVOLUTION; DNA SEQUENCE; ESCHERICHIA COLI; GENE DOSAGE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PLASMID;

BASE SEQUENCE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE DOSAGE; HUMANS; MUTAGENESIS; PLASMIDS; PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, BACTERIAL;

EID: 84855908601     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-483-4_3     Document Type: Article

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