A new method for rapid identification of ansamycin compounds by inactivating KLM gene clusters in potential ansamycin-producing actinomyces

Journal of Applied Microbiology

Volumn 112, Issue 2, 2012, Pages 353-362

  Wang, G ^a,b   Zhang, H ^b   Sun, G ^b   Wu, L ^b   Zhang, J ^a   Wang, Y ^b  

^a NANJING FORESTRY UNIVERSITY   (China)

^b BEIJING HOSPITAL   (China)

Author keywords

'universal targeting vector'; AHBA KLM gene cluster; Ansamycin; Red ET recombination

Indexed keywords

BACTERIA; STRAIN; VECTORS;

'UNIVERSAL TARGETING VECTOR'; 3-AMINO-5-HYDROXYBENZOIC ACID-KLM GENE CLUSTER; ACTINOMYCES; ANSAMYCINS; CONSERVED REGIONS; GENE CLUSTERS; HYDROXYBENZOIC ACIDS; OXIDO-REDUCTASES; RAPID IDENTIFICATION; RED/ET RECOMBINATION;

GENE ENCODING;

3 AMINO 5 HYDROXYBENZOIC ACID OXIDOREDUCTASE; 3 AMINO 5 HYDROXYBENZOIC ACID PHOSPHATASE; 3 AMINO 5 HYDROXYBENZOIC ACID SYNTHASE; ANSAMYCIN DERIVATIVE; BACTERIAL ENZYME; BACTERIAL PROTEIN; GELDANAMYCIN; KANAMYCIN; KLM PROTEIN; STREPTOVARICIN; UNCLASSIFIED DRUG;

ANTIBIOTICS; BACTERIUM; BIOMARKER; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; IDENTIFICATION METHOD; METABOLITE; MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION;

ACTINOMYCES; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; DRUG IDENTIFICATION; GENE CLUSTER; GENE CONSTRUCT; GENE INACTIVATION; GENE INSERTION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ACTINOBACTERIA (CLASS); ACTINOMYCES;

EID: 84855889688     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05206.x     Document Type: Article

References (26)

1. August, P.R., Tang, L., Yoon, Y.J., Ning, S., Müller, R., Yu, T.W., Taylor, M., Hoffmann, D. et al. (1998) Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. Chem Biol 5, 69-79.
2. Barr, R., Henninger, M.D. and Crane, F.L. (1967) Comparative studies on plastoquinone II Analysis for plastoquinones A, B, C and D. Plant Physiol 42, 1246-1254.
3. Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Rao, R.N. and Schoner, B.E. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116, 43-49.
4. Chen, S., Bamberg, D.V., Hale, V., Breuer, M., Hardt, B., Müller, R., Floss, H.G., Reynolds, K.A. et al. (1999) Biosynthesis of ansatrienin (mycotrienin) and naphthomycin. Identification and analysis of two separate biosynthetic gene clusters in Streptomyces collinus Tü 1892. Eur J Biochem 261, 98-107.
5. DeBoer, C., Meulman, P.A., Wnuk, R.J. and Peterson, D.H. (1970) Geldanamycin, a new antibiotic. J Antibiot 23, 442-447.
6. Fauci, A.S. (2001) Infectious diseases: considerations for the 21st century. Clin Infect Dis 32, 675-685.
7. Gao, Q.J., Shang, G.D., Yang, Y., Sun, G.Z. and Wang, Y.G. (2002) Cloning and molecular analysis of geldanamycin biosynthetic genes from Streptomyces hygroscopicus. Chin J Antibiot 27, 13-17.
8. He, W.Q., Wu, L.Z., Gao, Q.J., Du, Y. and Wang, Y.G. (2006) Identification of AHBA biosynthetic genes related to geldanamycin biosythesis in Streptomyces hygroscopicus 17997. Curr Microbiol 52, 197-203.
9. Hiramoto, S., Sugita, M., Ando, C., Sasaki, T., Furihata, K., Seto, H. and Otake, N. (1985) Studies on mycotrienin antibiotics, a novel class of ansamycins.V. Isolation and structure determination of novel mycotrienin congeners. J Antibiot 38, 1103-1106.
10. Keepers, Y.P., Pizao, P.E., Peters, G.J., Ark- Otte, J.V., Winograd, B. and Pinedo, H.M. (1991) Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing. Eur J Cancer Clin Oncol 27, 897-900.
11. Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics. Norwich, UK: John Innes Centre.
12. Kim, C.G., Yu, T.W., Fryhle, C.B., Handa, S. and Floss, H.G. (1998) 3-Amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics. J Biol Chem 273, 6030-6040.
13. Kim, C.G., Lamichhane, J., Song, K.I., Nguyen, V.D., Kim, D.H., Jeong, T.S., Kang, S.H., Kim, K.W. et al. (2008) Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. Arch Microbiol 189, 463-473.
14. Kishi, T., Yamana, H., Muroi, M., Harada, S. and Asai, M. (1972) Tolypomycin, a new antibiotic. III. Isolation and characterization of tolypomycin Y. J Antibiot 25, 11-15.
15. Lal, R. and Lal, S. (1994) Recent trends in rifamycin research. Bioessays 16, 211-216.
16. Lazar, G., Zähner, H., Damberg, M. and Zeeck, A. (1983) Ansatrienin A2 and A3: minor components of the ansamycin complex produced by Streptomyces collinus. J Antibiot 36, 187-189.
17. Li, Y.H., He, W.Q., Wang, Y.C., Wang, Y.G. and Shao, R.G. (2008) A new post-PKS modification process in the carbamoyltransferase gene inactivation strain of Streptomyces hygroscopicus 17997. J Antibiot 61, 347-355.
18. Mochizuki, J., Kobayashi, E., Furihata, K., Kawaguchi, A., Seto, H. and Take, N. (1986) New ansamycin antibiotics naphthoquinomycins A and B: inhibitors of fatty acid synthesis in Escherichia coli. J Antibiot 39, 157-161.
19. Ooka, K., Tanaka, M., Ishikawa, T. and Kato, F. (1999) An antimutagenic metabolite, streptovaricin C, isolated from Streptomyces sp. Biol Pharm Bull 22, 107-110.
20. Rascher, A., Hu, Z., Buchanan, G.O., Reid, R. and Hutchinson, C.H. (2005) Insight into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. Appl Environ Microbiol 71, 4862-4871.
21. Sohng, J.K., Oh, T.J., Lee, J.J. and Kim, G.G. (1997) Identification of a gene cluster of biosynthetic genes of rubradirin substrures in S. achromogenes var. rubradiris NRRL3061. Mol Cells 7, 674-681.
22. Tanida, S., Izawa, M. and Hasegawa, T. (1981) Ansamitocin analogs from a mutant strain of Nocardia.I. Isolation of the mutant, fermentation and antimicrobial properties. J Antibiot 34, 489-495.
23. Yu, T.W., Müller, R., Müller, M., Zhang, X., Draeger, G., Kim, C.G., Leistner, E. and Floss, H.G. (2001) Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in Amycolaptosis mediterranei S699. J Biol Chem 276, 12546-12555.
24. Yu, T.W., Bai, L., Clade, D., Hoffmann, D., Toelzer, S., Trinh, K.Q., Xu, J., Moss, S.J. et al. (2002) The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. Proc Natl Acad Sci USA 99, 7968-7973.
25. Zhang, M.Q., Gaisser, S., Nur-E-Alam, M., Sheehan, L.S., Vousden, W.A., Gaitatzis, N., Peck, G., Coates, N.J. et al. (2008) Optimizing natural products by biosynthetic engineering: discovery of nonquinone Hsp90 inhibitors. J Med Chem 51, 5494-5497.
26. Zhang, H.T., Wu, L.Z., Liu, A.M., Sun, G.Z., Hong, F., Liu, Q.P., Wu, Y.Z., Xia, H.Z. et al. (2009) PCR screening of 3-amino-5-hydroxybenzonic acid synthase gene leads to identification of ansamycins and AHBA-related antibiotic producers in Actinomycetes. J Appl Microbiol 106, 755-763.