Microscale affinity purification of trypsin reduces background peptides in matrix-assisted laser desorption/ionization mass spectrometry of protein digests

Journal of Proteomics

Volumn 74, Issue 7, 2011, Pages 948-957

  Chamrád, Ivo ^a   Strouhal, Ondřej ^a   Řehulka, Pavel ^b   Lenobel, René ^a   Šebela, Marek ^a  

^a PALACKÝ UNIVERSITY   (Czech Republic)

^b UNIVERSITY OF DEFENCE   (Czech Republic)

Author keywords

Affinity chromatography; Arginine; Autolysis; Benzamidine; Digestion; Trypsin

Indexed keywords

AMMONIA; ARGININE; BENZAMIDINE; BICARBONATE; BUFFER; ENZYME; HYDROCHLORIC ACID; LIGAND; PEPTIDE; PROTEIN; SEPHAROSE; SORBENT; TENIDAP; TRYPSIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; AUTOLYSIS; BINDING KINETICS; CONTROLLED STUDY; FINGER DERMATOGLYPHICS; IONIZATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; STANDARD; THERMOSTABILITY;

ANIMALS; CATTLE; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MICROCHEMISTRY; PEPTIDE MAPPING; PROTEIN HYDROLYSATES; PROTEINURIA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

BOVINAE;

EID: 79957655756     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2011.02.011     Document Type: Article

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