Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer

Methods in Molecular Biology

Volumn 833, Issue , 2012, Pages 337-349

  Shahian, Tina ^a   Narlikar, Geeta J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

ACF; ATP dependent chromatin remodeling; Histone; SNF2h

Indexed keywords

DNA; FLUORESCENT DYE; HISTONE;

ARTICLE; BIOLOGICAL MODEL; CHEMISTRY; CHROMATIN ASSEMBLY AND DISASSEMBLY; CONFORMATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; NUCLEOSOME; POLYMERASE CHAIN REACTION; STAINING; STATISTICS;

CHROMATIN ASSEMBLY AND DISASSEMBLY; DNA; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HISTONES; HUMANS; KINETICS; MODELS, BIOLOGICAL; NUCLEIC ACID CONFORMATION; NUCLEOSOMES; POLYMERASE CHAIN REACTION; STAINING AND LABELING; STATISTICS AS TOPIC;

EID: 84855870494     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-477-3_20     Document Type: Article

References (12)

1. Clapier CR, Cairns BR: The biology of chromatin remodeling complexes. Annu Rev Biochem 2009, 78:273-304.
2. Lakowicz J: Principles of Fluoroscence Spectroscopy. : Springer; 1999.
3. Stryer L: Intramolecular resonance transfer of energy in proteins. Biochim Biophys Acta 1959, 35:242-244.
4. Yang JG, Narlikar GJ: FRET-based methods to study ATP-dependent changes in chromatin structure. Methods 2007, 41:291-295.
5. Luger K, Mader AW, Richmond RK, Sargent DF, Richmond TJ: Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature 1997, 389:251-260. (Pubitemid 27406632)
6. Davey CA, Sargent DF, Luger K, Maeder AW, Richmond TJ: Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution. J Mol Biol 2002, 319: 1097-1113. (Pubitemid 34729421)
7. Park YJ, Dyer PN, Tremethick DJ, Luger K: A new fl uorescence resonance energy transfer approach demonstrates that the histone variant H2AZ stabilizes the histone octamer within the nucleosome. J Biol Chem 2004, 279: 24274-24282. (Pubitemid 38725289)
8. Li G, Levitus M, Bustamante C, Widom J: Rapid spontaneous accessibility of nucleosomal DNA. Nat Struct Mol Biol 2005, 12:46-53. (Pubitemid 40082916)
9. Yang JG, Madrid TS, Sevastopoulos E, Narlikar GJ: The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing. Nat Struct Mol Biol 2006, 13:1078-1083. (Pubitemid 44885917)
10. Dyer PN, Edayathumangalam RS, White CL, Bao Y, Chakravarthy S, Muthurajan UM, Luger K: Reconstitution of nucleosome core particles from recombinant histones and DNA. Methods Enzymol 2004, 375:23-44. (Pubitemid 38124858)
11. Luger K, Rechsteiner TJ, Richmond TJ: Expression and purification of recombinant histones and nucleosome reconstitution. Methods Mol Biol 1999, 119:1-16.
12. Lee KM, Narlikar G: Assembly of nucleosomal templates by salt dialysis. Curr Protoc Mol Biol 2001, Chapter 21:Unit 21 26.