A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis

PLoS ONE

Volumn 7, Issue 1, 2012, Pages

  Xu, Wentao ^a,b   Zhai, Zhifang ^a   Huang, Kunlun ^a,b   Zhang, Nan ^b   Yuan, Yanfang ^a   Shang, Ying ^b   Luo, Yunbo ^a,b  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; PLANT DNA; PRIMER DNA; VEGETABLE PROTEIN;

ARTICLE; CONTROLLED STUDY; COTTON; DNA SEQUENCE; DNA TEMPLATE; GEL ELECTROPHORESIS; GENE AMPLIFICATION; INHIBITION KINETICS; LIMIT OF DETECTION; MAIZE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PAPAYA; PROCESS OPTIMIZATION; RAPESEED; RICE; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCING GEL ELECTROPHORESIS; SOYBEAN; TRANSGENIC CROP; UNIVERSAL PRIMER MULTIPLEX POLYMERASE CHAIN REACTION; CARICA; ELECTROPHORESIS; GENETICS; GOSSYPIUM; METHODOLOGY; REPRODUCIBILITY; TRANSGENIC PLANT;

BRASSICA NAPUS; CARICA; DNA PRIMERS; DNA, PLANT; ELECTROPHORESIS; GOSSYPIUM; MULTIPLEX POLYMERASE CHAIN REACTION; ORYZA SATIVA; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA; SOYBEANS; ZEA MAYS;

EID: 84855827286     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0022900     Document Type: Article

References (27)

1. Zhang YL, Zhang DB, Li WQ, Chen JQ, Peng YF, et al. (2003) A novel real-time quantitative PCR method using attached universal template probe. Nucleic Acids Res 31: e123.
2. Shrestha HK, Hwu K, Wang S, Liu L, Chang M, (2008) Simultaneous detection of eight genetically modified maize lines using a combination of event- and construct-specific multiplex-PCR technique. J Agric Food Chem 56: 8962-8968.
3. Pinar A, Bozdemir N, Kocagoz T, Alacam R, (2004) Rapid detection of bacterial atypical pneumonia agents by multiplex PCR. Cent Eur J Public Health 12: 3-5.
4. Ding C, Cantor CR, (2003) A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight. MSProc Natl Acad Sci 100: 3059-3064.
5. Hess CJ, Denkers F, Ossenkoppele GJ, Waisfisz Q, McElgunn CJ, et al. (2004) Gene expression profiling of minimal residual disease in acute myeloid leukaemia by novel multiplex-PCR-based method. Leukemia 18: 1981-1988.
6. Tettelin H, Radune D, Kasif S, Khouri H, Salzberg S, (1999) Optimized multiplex PCR: efficiently closing a whole-genome shotgun sequencing project. Genomics 62: 500-507.
7. Inagaki S, Yamamoto Y, Doi Y, Takata T, Ishikawa T, et al. (2004) A new 39-plex analysis method for SNPs including 15 blood group loci. Forensic Sci Int 144: 45-57.
8. Elnifro E, Ashshi A, Cooper R, Klapper P, (2000) Multiplex PCR: optimization and application in diagnostic virology. Clin Microbiol Rev 13: 559-570.
9. Shi M, Bleavins M, de la Iglesia F, (1999) Technologies for detecting genetic polymorphisms in pharmacogenomics. Mol Diagn 4: 343-351.
10. Shi MM, (2001) Enabling large-scale pharmacogenetic studies by high-throughput mutation detection and genotyping technologies. Clinical Chemistry 47: 164-72.
11. James D, Schmidt A, Wall E, Green M, Masri S, (2003) Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis. J Agric Food Chem 51: 5829-5834.
12. Zhang MH, Gao XJ, Yu YB, Qin J, Li QZ, (2007) Detection of Roundup Ready soy in highly processed products by triplex nested PCR. Food Control 18 (10): 1277-1281.
13. Randhawa GJ, Chhabra R, Singh M, (2009) Multiplex PCR-Based Simultaneous Amplification of Selectable Marker and Reporter Genes for the Screening of Genetically Modified Crops. J Agric Food Chem 57: 5167-5172.
14. Randhawa GJ, Chhabra R, Singh M, (2008) Molecular characterization of Bt cauliflower with Multiplex PCR and validation of endogenous reference gene in Brassicaceae family. Curr Sci 95 (12): 1729-1731.
15. Randhawa GJ, Singh M, Chhabra R, Guleria S, Sharma R, (2009) Molecular diagnosis of transgenic tomato with osmotin gene using multiplex polymerase chain reaction. Curr Sci 96 (5): 689-694.
16. Randhawa GJ, Sharma R, Singh M, (2009) Multiplex polymerase chain reaction for detection of genetically modified potato (Solanum tuberosum L.) with cry1Ab gene. Indian J Agric Sci 79 (5): 368-371.
17. Bai WB, Xu WT, Huang KL, Yuan YF, Cao SS, et al. (2009) A novel common primer multiplex PCR (CP-M-PCR) method for the simultaneous detection of meat species. Food Control 20: 366-370.
18. Yuan YF, Xu WT, Zhai ZF, Shi H, Luo YB, et al. (2009) Universal Primer-Multiplex PCR approach for simultaneous detection of Escherichia coli, Listeria monocytogenes, and Salmonella spp. in food samples. Food Microbio Saf 74 (8): 446-452.
19. Xu WT, Yuan YF, Luo YB, Bai WB, Zhang CJ, et al. (2009) Event-specific detection of stacked genetically modified maize Bt11×GA21 by UP-M-PCR and real-time PCR. J Agric Food Chem 57: 395-402.
20. Brant JB, Peter MG, (2007) Silver staining DNA in polyacrylamide gels. Nat Protoc 2 (11): 2649-2654.
21. Sambrook J, Russel DW, (2001) Molecular Cloning: A Laboratory Manual 3rd Ed Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Heukeshoven J, Dernick R, (1985) Simplified method for staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6: 103-112.
23. Somerville LL, Wang K, (1981) The ultrasensitive silver 'protein' stain also detects nanograms of nucleic acids. Biochem. Biophys Res Commun 102: 53-58.
24. Boulikas T, Hancock R, (1981) A highly sensitive technique for staining DNA and RNA in polyacrylamide gels using silver. J Biochem Biophys Methods 4: 219-228.
25. Junichi M, Taichi O, Hiroshi A, Reiko T, Akihiro H, et al. (2009) Simultaneous detection of recombinant DNA segments introduced into genetically modified crops with multiplex ligase chain reaction coupled with Multiplex Polymerase Chain Reaction. J Agric Food Chem 57 (7): 2640-2646.
26. Tomoaki Y, Hideo K, Takeshi M, Takashi K, Mayu I, et al. (2005) Applicability of quantification of genetically modified organisms to foods processed from maize and soy. J Agric Food Chem 53: 2052-2059.
27. Tigst D, Indira R, (2008) Multiplex qualitative PCR assay for identification of genetically modified canola events and real-time event-specific PCR assay for quantification of the GT73 canola event. Food Control 19: 893-897.