Susceptibility of lupin γ-conglutin, the plasma glucoselowering protein of lupin seeds, to proteolytic enzymes

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 18, 2009, Pages 8612-8616

  Capraro, Jessica ^a   Magni, Chiara ^a   Scarafoni, Alessio ^a   Duranti, Marcello ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Conglutln; Luplnus albus; Ph; Protease susceptibility

Indexed keywords

ANTIDIABETIC AGENT; BROMELAIN; PANCREATIN; PEPSIN A; PEPTIDE HYDROLASE; SEED STORAGE PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LUPIN; METABOLISM; PH; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BROMELAINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; HYPOGLYCEMIC AGENTS; LUPINUS; PANCREATIN; PEPSIN A; PEPTIDE HYDROLASES; PLANT PROTEINS; SEED STORAGE PROTEINS; SEEDS;

ANIMALIA;

EID: 70349305387     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9017542     Document Type: Article

References (22)

1. Duranti, M.; Restani, P.; Poniatowska, M.; Cerletti, P. The seed globulins of Lupinus albus. Phytochemistry 1981, 20, 2071-2075.
2. Restani, P.; Duranti, M.; Cerletti, P.; Simonetti, P. Subunit composition of the seed globulins of Lupinus albus. Phytochemistry 1981, 20, 2077-2083.
3. Scarafoni, A.; Di Cataldo, A.; Vassilevskaia, T. D.; Bekman, E. P.; Rodrigues-Pousada, C.; Ceciliani, F.; Duranti, M. Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin y genes. Biochim. Biophis. Acta 2001, 1519, 147-151.
4. Kolivas, S.; Gayler, K. R. Structure of the cDNA coding for conglutin γ, a sulphur-rich protein from Lupinus angustifolius. Plant Mol Biol. 1993, 21, 397-401.
5. (5) Duranti, M.; Faoro, F.; Harris, N. The unusual extracellular localization of conglutin γ in germinating Lupinus albus seeds rules out its role as a storage protein. J. Plant Physiol. 1994, 143, 711-716. (Pubitemid 2099880)
6. Magni, C.; Sessa, F.; Accardo, E.; Vanoni, M.; Morazzoni, P.; Scarafoni, A.; Duranti, M. Conglutin γ, a lupin seed protein, binds insulin in vitro and reduces plasma glucose levels of hyperglycemic rats. J. Nutr. Biochem. 2004, 15, 646-650.
7. (7) Clemente, A.; Vioque, J.; Sanchez-Vioque, R.; Bautista, J.; Millan, F. Factors affecting the in vitro digestibility of chickpea albumins. J. Sci. Food Agric. 2000, 80, 79-84. (Pubitemid 30065433)
8. Hausch, F.; Shan, L.; Santiago, N. A.; Gray, G. M.; Khosla, C. Intestinal digestive resistance of immunodominant gliadin peptides. Am. J. Physiol. 2002, 283, G996-G1003.
9. Rhodes, J. M.; Campbell, B. J.; Yu, L.-G. Lectin-epithelial interactions in the human colon. Biochem. Soc. Trans. 2008, 36, 1482-1486.
10. Moreno, J.; Clemente, A. 2S Albumin storage proteins: What makes them food allergens? Open Biochem. J. 2008, 2, 16-28.
11. Duranti, M.; Gius, C.; Sessa, F.; Vecchio, G. The saccharide chain of lupin seed conglutin gamma is not responsible for the protection of the native protein from degradation by trypsin, but facilitates the refolding of the acid-treated protein to the resistant conformation. Eur. J. Biochem. 1995, 230, 886-891.
12. (12) Duranti, M.; Scarafoni, A.; Gius, C.; Faoro, F.; Negri, A. Heat induced synthesis and tunicamycin-secretion of the putative storage glycoprotein conglutin γ from mature lupin seeds. Eur. J. Biochem. 1994, 222, 387-393. (Pubitemid 24204907)
13. Gasteiger, E.; Hoogland, C.; Gattiker, A., Duvaud, S.; Wilkins, M. R.; Appel, M. R.; Bairoch, A. Protein identification and analysis tools on the ExPASy server. In The Proteomics Protocols Handbook; Walker, J. M., Ed.; Humana Press: Totova, NJ, 2005; pp 571-608.
14. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
15. Schlamowitz, M.; Peterson, L. U. Studies on the optimum pH for the action of pepsin on "native" and denatured bovine serum albumin and bovine hemoglobin. J. Biol. Chem. 1959, 234, 3137-3145.
16. Harrach, T.; Eckert, K.; Maurer, H. R.; Machleidt, I.; Machleidt, W.; Nuck, R. Isolation and characterization of two forms of an acidic bromelain stem proteinase. J. Protein Chem. 1998, 17, 351-361.
17. Strickland, E. H. Aromatic contribution to circular dichroism spectra of proteins. CRC Crit. Rev. Biochem. 1974, 5, 113-175.
18. Kelly, S. M.; Jess, T. J.; Price, N. C. How to study proteins by circular dichroism. Biochim. Biophys. Acta 2005, 1751, 119-139.
19. (19) Polverino De Laureto, P.; Frare, E.; Gottardo, R.; Van Dael, H.; Fontana, A. Partly folded states of members of the lysozyme/ lactalbumin superfamily: A comparative study by circular dichroism spectroscopy and limited proteolysis. Protein Sci. 2002, 11, 2932-2946. (Pubitemid 35365259)
20. (20) Duranti, M.; Sessa, F.; Scarafoni, A.; Bellini, T.; Dallocchio, F. Thermal stabilities of lupin seed conglutin γ protomers and tetramers. J. Agric. Food Chem. 2000, 48, 1118-1123. (Pubitemid 30243672)
21. Terruzzi, I.; Senesi, P.; Magni, C.; Montesano, A.; Scarafoni, A.; Luzi, A.; Duranti, M. Insulin-mimetic action of conglutin-γ, a lupin seed protein, in mouse myoblasts. Nutr., Metab. Cardiovase Dis. 2009, manuscript submitted.
22. Foss, N.; Duranti, M.; Magni, C.; Frakiaer, H. Assessment of lupin allergenicity in the cholera toxin model: Induction of IgE response depends on the intrinsic properties of the conglutins and matrix effects. Int. Arch. Allergy Immunol. 2006, 141, 141-150.