Production of UCP1 a membrane protein from the inner mitochondrial membrane using the cell free expression system in the presence of a fluorinated surfactant

Biochimica et Biophysica Acta - Biomembranes

Volumn 1818, Issue 3, 2012, Pages 798-805

  Blesneac, Iulia ^a,b,c,h   Ravaud, Stéphanie ^a,b,c   Juillan Binard, Céline ^a,b,c   Barret, Laure Anne ^d,e   Zoonens, Manuela ^f,g   Polidori, Ange ^d,e   Miroux, Bruno ^f,g   Pucci, Bernard ^d,e   Pebay Peyroula, Eva ^a,b,c  

^a UNIV GRENOBLE ALPES   (France)

^b CEA GRENOBLE   (France)

^c INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^d UNIVERSITY OF AVIGNON   (France)

^e UNIVERSITÉ DE MONTPELLIER   (France)

^f UNIVERSITÉ PARIS DESCARTES   (France)

^g PSL Research University   (France)

^h UNIV MONTPELLIER   (France)

Author keywords

Cell free expression; Circular dichroism; Fluorinated surfactants; Membrane protein expression; Mitochondrial carriers; Uncoupling protein

Indexed keywords

FLUORINATED SURFACTANT; SURFACTANT; UNCLASSIFIED DRUG; UNCOUPLING PROTEIN 1;

ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; INNER MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL MEMBRANE; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SOLUBILITY; THERMOSTABILITY;

ANIMALS; CATTLE; CELL-FREE SYSTEM; ESCHERICHIA COLI; GENE EXPRESSION; HYDROCARBONS, FLUORINATED; ION CHANNELS; MITOCHONDRIAL MEMBRANES; MITOCHONDRIAL PROTEINS; RATS; RECOMBINANT PROTEINS; SURFACE-ACTIVE AGENTS;

BOVINAE; PROKARYOTA;

EID: 84855784885     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2011.12.016     Document Type: Article

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