Identification by site-directed mutagenesis of three arginines in uncoupling protein that are essential for nucleotide binding and inhibition

Journal of Biological Chemistry

Volumn 272, Issue 40, 1997, Pages 24759-24762

  Modrianský, Martin ^a   Murdza Inglis, Debra L ^b   Patel, Hasmukh V ^b   Freeman, Karl B ^b   Garlid, Keith D ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; FATTY ACID; HISTIDINE; LIPOSOME;

ALLOSTERISM; ARTICLE; CONFORMATIONAL TRANSITION; DISSOCIATION; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS;

ANIMALS; ARGININE; BINDING SITES; CARRIER PROTEINS; CODON; GUANOSINE DIPHOSPHATE; ION CHANNELS; LYSINE; MEMBRANE PROTEINS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; MODELS, STRUCTURAL; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; RATS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 0030881775     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.40.24759     Document Type: Article

References (29)

1. Klingenberg, M. (1990) Trends Biochem. Sci. 15, 108-112
2. Nicholls, D. G. (1979) Biochim. Biophys. Acta 549, 1-22
3. Garlid, K. D., Orosz, D. E., Modriansky, M., Vassanelli, S., and Jezek, P. (1996) J. Biol. Chem. 271, 2615-2620
4. Jezek, P., Orosz, D. E., Modriansky, M., and Garlid, K. D. (1994) J. Biol. Chem. 269, 26184-26190
5. Winkler, E., and Klingenberg, M. (1992) Eur. J. Biochem. 203, 295-304
6. Mayinger, P., and Klingenberg, M. (1992) Biochemistry 31, 10536-10543
7. Katiyar, S. S., and Shrago, E. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 2559-2562
8. Murdza-Inglis, D. L., Patel, H. V., Freeman, K. B., Jezek, P., Orosz, D. E., and Garlid, K. D. (1991) J. Biol. Chem. 266, 11871-11875
9. Murdza-Inglis, D. L., Modriansky, M., Patel, H. V., Woldegiorgis, G., Freeman, K. B., and Garlid, K. D. (1994) J. Biol. Chem. 269, 7435-7438
10. Aquila, H., Link, T. A., and Klingenberg, M. (1985) EMBO J. 4, 2369-2376
11. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 488-492
12. Gasser, S. M. (1983) Methods Enzymol. 97, 329-336
13. Garlid, K. D., Sun, X., Paucek, P., and Woldegiorgis, G. (1995) Methods Enzymol. 260, 331-348
14. Orosz, D. E., and Garlid, K. D. (1993) Anal. Biochem. 210, 7-15
15. Kaplan, R. S., and Pedersen, P. L. (1985) Anal. Biochem. 150, 97-104
16. Klingenberg, M., Herlt, M., and Winkler, E. (1986) Methods Enzymol. 126, 498-504
17. Jezek, P., Orosz, D. E., and Garlid, K. D. (1990) J. Biol. Chem. 265, 19296-19302
18. Murdza-Inglis, D. L. (1995) Structure/Function Studies of Rat Uncoupling Protein in Yeast Mitochondria (Energy Metabolism). Ph.D. thesis, McMaster University, Hamilton, Canada
19. Huang, S.-G., and Klingenberg, M. (1996) Biochemistry 35, 7846-7854
20. Rafael, J., Pampel, I., and Wang, X. (1994) Eur. J. Biochem. 223, 971-980
21. Huang, S.-G., and Klingenberg, M. (1995) Biochemistry 34, 349-360
22. Fleury, C., Neverova, M., Collins, S., Raimbault, S., Champigny, O., LeviMeyrueis, C., Bouillaud, F., Seldin, M. F., Surwit, R. S., Ricquier, D., and Warden, C. H. (1997) Nat. Genet. 15, 269-273
23. Gimeno, R. E., Dembski, M., Weng, X., Deng, N., Shyjan, A. W., Gimeno, C. J., Iris, F., Ellis, S. J., Woolf, E. A., and Tartaglia, L. A. (1997) Diabetes 46, 900-906
24. Klingenberg, M. (1988) Biochemistry 27, 781-791
25. Winkler, E., Wachter, E., and Klingenberg, M. (1997) Biochemistry 36, 148-155
26. Lin, C., and Klingenberg, M. (1982) Biochemistry 21, 2950-2956
27. Nicholls, D. G., Cannon, B., Grav, H. J., and Lindberg, O. (1974) in Dynamics of Energy-transducing Membranes (Ernster, L., ed) pp. 529-537, Elsevier Science Publishers B. V., Amsterdam
28. Garlid, K. D. (1990) Biochim. Biophys. Acta 1018, 151-154
29. Miroux, B., Frossard, V., Raimbault, S., Ricquier, D., and Bouillaud, F. (1993) EMBO J. 12, 3739-3745