Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H+ uniport

International Journal of Biochemistry and Cell Biology

Volumn 35, Issue 2, 2003, Pages 212-220

  Urbánková, Eva ^a   Hanák, Petr ^a   Škobisová, Eva ^a   Růžička, Michal ^a   Ježek, Petr ^a  

^a INSTITUTE OF PHYSIOLOGY   (Czech Republic)

Author keywords

Fatty acid induced H+ uniport; Mitochondrial uncoupling protein UCP1; Site directed mutagenesis

Indexed keywords

ASPARTIC ACID; FATTY ACID; LAURIC ACID; UNCOUPLING PROTEIN 1; CARRIER PROTEIN; CHLORIDE; GUANOSINE TRIPHOSPHATE; HYDROGEN; ION CHANNEL; LAURIC ACID DERIVATIVE; LIPOSOME; MEMBRANE PROTEIN; MITOCHONDRIAL PROTEIN; MITOCHONDRIAL UNCOUPLING PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; KINETICS; MITOCHONDRION; MUTATION; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS; AMINO ACID SUBSTITUTION; ANIMAL; CHEMISTRY; DRUG EFFECT; GENETICS; ION TRANSPORT; METABOLISM; PHYSIOLOGY; RAT; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; ANIMALS; CARRIER PROTEINS; CHLORIDES; FATTY ACIDS; GUANOSINE TRIPHOSPHATE; HYDROGEN; ION CHANNELS; ION TRANSPORT; KINETICS; LAURIC ACIDS; LIPOSOMES; MEMBRANE PROTEINS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; MUTAGENESIS, SITE-DIRECTED; RATS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0037290649     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(02)00131-0     Document Type: Article

References (33)

1. Klingenberg M., Huang S.-G. Structure and function of the uncoupling protein from brown adipose tissue. Biochim. Biophys Acta. 1415:1999;271-296.
2. Ježek P. Fatty acid interaction with mitochondrial uncoupling proteins. J. Bioenerg. Biomembr. 31:1999;457-466.
3. Ricquier D., Bouillaud F. The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP. Biochem. J. 345:2000;161-179.
4. Ježek P. Possible physiological roles of mitochondrial uncoupling proteins: UCPn. Int. J. Biochem. Cell Biol. 34:2002;1190-1206.
5. Ježek P., Urbánková E. Specific sequence motifs of mitochondrial uncoupling proteins. IUBMB Life. 49:2000;63-70.
6. Hanák P., Ježek P. Mitochondrial uncoupling protein and phylogenesis: UCP4 as the ancestral uncoupling protein. FEBS Lett. 495:2001;137-141.
7. Walker J.E., Runswick M.J. The mitochondrial transport protein superfamily. J. Bioenerg. Biomembr. 25:1993;435-446.
8. El Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E. Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae. Yeast. 13:1997;573-581.
9. Aquila H., Link T.A., Klingenberg M. The uncoupling protein from brown fat mitochondria is related to the mitochondrial ADP/ATP carrier: analysis of sequence homologies and of folding of the protein in the membrane. EMBO J. 4:1985;2369-2376.
10. Echtay K.S., Bienengraeber M., Klingenberg M. Mutagenesis of the uncoupling protein of brown adipose tissue: neutralization of E190 largely abolishes pH control of nucleotide binding. Biochemistry. 36:1997;8253-8260.
11. Echtay K.S., Bienengraeber M., Winkler E., Klingenberg M. In the uncoupling protein (UCP-1) His214 is involved in the regulation of purine nucleoside triphosphate but not diphosphate binding. J. Biol. Chem. 273:1998;24368-243374.
12. + transport by uncoupling protein (UCP-1) is dependent on a histidine pair absent in UCP-2 and UCP-3. Biochemistry. 37:1998;3-8.
13. Echtay K.S., Winkler E., Bienengraeber M., Klingenberg M. Site-directed mutagenesis identifies residues in uncoupling protein (UCP1) involved in three different functions. Biochemistry. 39:2000;3311-3317.
14. Echtay K.S., Bienengraeber M., Klingenberg M. Role of intrahelical arginine residues in functional properties of uncoupling protein (UCP1). Biochemistry. 40:2001;4248-5243.
15. Arechaga I., Raimbault S., Prieto S., Levi-Meyrueis C., Zaragoza P., Miroux B., Ricquier D., Bouillaud F., Rial E. Cysteine residues are not essential for uncoupling protein function. Biochem. J. 296:1993;693-700.
16. Bouillaud F., Arechaga I., Petit P.X., Raimbault S., Levi-Meyrrueis C., Casteilla L., Laurent M., Rial E., Ricquier D. A sequence related to a DNA recognition element is essential for inhibition by nucleotides of proton transport through the mitochondrial uncoupling protein. EMBO J. 13:1994;1990-1997.
17. Gonzáles-Barroso M.-M., Fleury C., Arechaga I., Zaragoza P., Levi-Meyrrueis C., Raimbault S., Ricquier D., Bouillaud F., Rial E. Activation of the uncoupling protein by fatty acids is modulated by mutations in the C-terminal region of the protein. Eur. J. Biochem. 239:1996;445-450.
18. Gonzáles-Barroso M.-M., Fleury C., Jiménes M.A., Sanz J.M., Romero A., Bouillaud F., Rial E. Structural and functional study of a conserved region in the uncoupling protein UCP1: the three matrix loops are involved in the control of transport. J. Mol. Biol. 292:1999;137-149.
19. + transport. J. Biol. Chem. 269:1994;7435-7438.
20. Modrianský M., Murdza-Inglis D.L., Patel H.V., Freeman K.B., Garlid K.D. Identification by site-directed mutagenesis of three arginines in uncoupling protein that are essential for nucleotide binding and inhibition. J. Biol. Chem. 272:1997;24759-24762.
21. Winkler E., Wachter E., Klingenberg M. Identification of the pH sensor for nucleotide binding in the uncoupling protein from brown adipose tissue. Biochemistry. 36:1997;148-155.
22. Klingenberg M., Winkler E. Photoaffinity labeling of the nucleotide-binding site of the uncoupling protein from hamster brown adipose tissue. Eur. J. Biochem. 203:1992;295-304.
23. Mayinger P., Klingenberg M. Labeling of two different regions of the nucleotide binding site of the uncoupling protein from brown adipose tissue mitochondria with two ATP analogs. Biochemistry. 31:1992;10536-10543.
24. Klingenberg M. Nucleotide binding to uncoupling protein: mechanism of protonation. Biochemistry. 27:1988;781-791.
25. + transport inhibition of the uncoupling protein of brown adipose tissue mitochondria. Biochemistry. 35:1996;7846-7854.
26. Žáčková M., Krämer R., Ježek P. Interaction of mitochondrial phosphate carrier with fatty acids and hydrophobic phosphate analogs. Int. J. Biochem. Cell. Biol. 32:2000;499-508.
27. + translocator. EMBO J. 4:1985;3087-3092.
28. Garlid K.D., Orosz D.E., Modrianský M., Vassanelli S., Ježek P. On the mechanism of fatty acid-induced proton transport by mitochondrial uncoupling protein. J. Biol. Chem. 271:1996;2615-2620.
29. Ježek P., Orosz D.E., Modrianský M., Garlid K.D. Transport of anions and protons by the mitochondrial uncoupling protein and its regulation by nucleotides and fatty acids: a new look at old hypotheses. J. Biol. Chem. 269:1994;26184-26190.
30. Kaplan R.S., Pedersen P.L. Anal. Biochem. 150:1985;97-104.
31. Ježek P., Orosz D.E., Garlid K.D. Reconstitution of the uncoupling protein of brown adipose tissue mitochondria: demonstration of GDP-sensitive halide anion uniport. J. Biol. Chem. 265:1990;19296-19302.
32. Scatchard G. The attraction of proteins for small molecules and ions. Ann. New York Acad. Sci. 51:1949;660.
33. Hagen T., Lowell B.B. Chimeric proteins between UCP1 and UCP3: the middle third of UCP1 is necessary and sufficient for activation by fatty acids. Biochem. Biophys. Res. Commun. 276:2000;642-648.