A small interfering RNA targeting NF-κB p65 alone or combined with 5-FU inhibits growth of esophageal squamous cell carcinoma in nude mice

Pathology Research and Practice

Volumn 208, Issue 1, 2012, Pages 32-38

  Tian, Fang ^a   Fan, Tianli ^a   Jiang, Yanan ^a   Zhang, Xiaoyan ^a   Wang, Xinhua ^b  

^a ZHENGZHOU UNIVERSITY   (China)

^b THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIVERSITY   (China)

Author keywords

5 Fluorouraci; Esophageal squamous cell carcinoma (ESCC); NF B signaling pathway; Nude mice; SiRNA

Indexed keywords

FLUOROURACIL; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; SMALL INTERFERING RNA; SYNAPTOTAGMIN I;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER COMBINATION CHEMOTHERAPY; CANCER INHIBITION; CANCER SURVIVAL; CONTROLLED STUDY; DOWN REGULATION; DRUG CYTOTOXICITY; DRUG EFFECT; DRUG MECHANISM; DRUG POTENTIATION; ESOPHAGEAL SQUAMOUS CELL CARCINOMA; GENE EXPRESSION REGULATION; GENE SILENCING; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; MALE; MONOTHERAPY; MOUSE; NICK END LABELING; NONHUMAN; PROTEIN FUNCTION; PROTEIN TARGETING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ANIMALS; ANTINEOPLASTIC AGENTS; BLOTTING, WESTERN; CARCINOMA, SQUAMOUS CELL; CELL PROLIFERATION; DOWN-REGULATION; ESOPHAGEAL NEOPLASMS; FLUOROURACIL; GENE KNOCKDOWN TECHNIQUES; HUMANS; IMMUNOHISTOCHEMISTRY; IN SITU NICK-END LABELING; MALE; MICE; MICE, NUDE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTOR RELA; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 84855773615     PISSN: 03440338     EISSN: 16180631     Source Type: Journal    
DOI: 10.1016/j.prp.2011.10.011     Document Type: Article

References (27)

1. Aota K., Azuma M., Yamashita T., Tamatani T., Motegi K., Ishimaru N., Hayashi Y., Sato M. 5-Fluorouracil induced apoptosis through the suppression of NF-κB activity in human salivary gland cancer cells. Biochem. Biophys. Res. Commun. 2000, 273:1168-1174.
2. Chen F.E., Ghosh G. Regulation of DNA binding by Rel/NF-kappaB transcription factors: structural views. Oncogene 1999, 18:6845-6852.
3. Chi J.T., Chang H.Y., Wang N.N., Chang D.S., Dunphy N., Brown P.O. Genomewide view of gene silencing by small interfering RNAs. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:6343-6346.
4. Duffey D.C., Chen Z.H., Dong G., Ondrey F.G., Wolf J.S., Brown K., Siebenlist U., Van W.C. Expression of a dominant-negative mutant inhibitor-κB of nuclear factor-κB in human head and neck squamous cell carcinoma inhibits survival, proinflammatory cytokine expression, and tumor growth in vivo. Cancer Res. 1999, 59:3468-3475.
5. Guo K., Kang N.X., Li Y., Sun L., Gan L., Cui F.J., Gao M.D., Liu K.Y. Regulation of HSP27 on NF-kappaB pathway activation may be involved in metastatic hepatocellular carcinoma cells apoptosis. BMC Cancer 2009, 9:100-110.
6. Hannon G.J. A interference. Nature 2002, 418:244-251.
7. Hou G., Zhang Q., Wang L., Liu M., Wang J., Xue L. mTOR inhibitor rapamycin alone or combined with cisplatin inhibits growth of esophageal squamous cell carcinoma in nude mice. Cancer Lett. 2010, 290:248-254.
8. Jackson A.L., Bartz S.R., Schelter J., Kobayashi S.V., Burchard J., Mao M., Li B., Cavet G., Linsley P.S. Expression profiling revealsoff-target gene regulation by RNAi. Nat. Biotechnol. 2003, 21:635-637.
9. Liptay S., Weber C.K., Ludwig L., Wagner M., Adler G., Schmid R.M. Mitogenic and antiapoptotic role of constitutive NF-κB/Rel activity in pancreatic cancer. Int. J. Cancer 2003, 105:735-746.
10. Montesano R., Hollstein M., Hainaut P. Genetic alterations in esophageal cancer and their relevance to etiology and pathogenesis: a review. Int. J. Cancer 1996, 69:225-235.
11. Nair A., Venkatraman M., Maliekal T.T., Nair B., Karimagaram D. NF-κB is constitutively activated in high-grade squamous intraepithelial lesions and squamous cell carcinomas of the human uterine cervix. Oncogene 2003, 22:50-58.
12. Pahl H.L. Activators and target genes of Rel/NF-κB transcription factors. Oncogene 1999, 18:6853-6866.
13. Pisani P., Parkin D.M., Bray F., Ferlay J. Estimates of the worldwide mortality from 25 cancers in 1990. Int. J. Cancer 1999, 83:18-29.
14. Prasad S., Ravindran J., Aggarwal B.B. NF-kappaB and cancer: how intimate is this relationship. Mol. Cell. Biochem. 2010, 336:25-37.
15. Scherr M., Battmer K., Winkler T., Heidenreich O., Ganser A., Eder M. Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood 2003, 101:1566-1569.
16. Semizarov D., Frost L., Sarthy A., Kroeger P., Halbert D.N., Fesik S.W. Specificity of short interfering RNA determined through gene expression signatures. Proc. Natl. Acad. Sci. U.S.A. 2003, 11:6347-6352.
17. Sethi G., Ahn K.S., Sung B., Aggarwal B.B. Pinitol targets nuclear factor-kappaB activation pathway leading to inhibition of gene products associated with proliferation, apoptosis, invasion, and angiogenesis. Mol. Cancer Ther. 2008, 7:1604-1614.
18. Sethi G., Sung B., Aggarwal B.B. Nuclear factor-kappaB activation: from bench to bedside. Exp. Biol. Med. 2008, 233:21-31.
19. Sung B., Pandey M.K., Aggarwal B.B. Fisetin, an inhibitor of cyclin-dependent kinase 6, down-regulates nuclear factor-kappaB-regulated cell proliferation, antiapoptotic and metastatic gene products through the suppression of TAK-1 and receptor-interacting protein-regulated IkappaBalpha kinase activation. Mol. Pharmacol. 2007, 71:1703-1714.
20. Tamatani T., Azuma M., Ashida Y., Motegi K., Takashima R., Harada K., Kawaguchi S., Sato M. Enhanced radiosensitization and chemosensitization in NF-kB-suppressed human oral cancer cells via the inhibition of γ-irradiation- and 5-FU-induced production of IL-6and IL-8. Int. J. Cancer 2004, 108:912-921.
21. Tian F., Zang W.D., Xu P.R., Liu H.T., Xue L.X. Nuclear factor-kappaB signaling pathway constitutively activated in esophageal squamous cell carcinoma cell lines and inhibition of growth of cells by siRNA. Acta Biochim. Biophys. Sin. 2006, 38:318-326.
22. Tian F., Song M., Xu P.R., Liu H.T., Xue L.X. Effect of siRNA-mediated inhibition of nuclear factor-κB in combination with 5-FU on apoptosis of squamous cell carcinoma cell lines. World Chin. J. Digestol. 2008, 16:1716-1721.
23. Tian F., Chai Y.R., Xu P.R., Liu H.T., Xue L.X. Effect of siRNA-mediated Inhibition of NF-κB combination with 5-Fu on cell cycle of ESCC cell lines. Cancer Res. Prev. Treat. 2008, 35:617-620.
24. Tuschl T., Borkhardt A. Small interfering RNAs: a revolutionary tool for the analysis of gene function and gene therapy. Mol. Interventions 2002, 2:158-167.
25. Wohlbold L., van der Kuip H., Miething C., Vornlocher H.P., Knabbe C., Duyster J., Aulitzky W.E. Inhibition of bcr-abl gene expression by small interfering RNA sensitizes for imatinib mesylate (STI571). Blood 2003, 102:2236-2239.
26. Yu H.G., Yu L.L., Yang Y., Luo H.S., Yu J.P., Meier J.J., Schrader H., Bastian A., Schmidt W.E., Schmitz F. Increased expression of RelA/nuclear factor-kappa B protein correlates with colorectal tumorigenesis. Oncology 2003, 65:37-45.
27. Zhou M., Gu L.B., Zhu N.X., Woods W.G., Findley H.W. Transfection of a dominant-negative mutant NF-κB inhibitor (IκBm) represses p53-dependent apoptosis in acute lymphoblastic leukemia cells: interaction of IκBm and p53. Oncogene 2003, 22:8137-8144.