Real-time PCR for the detection of precise transgene copy number in durum wheat

Cellular and Molecular Biology Letters

Volumn 16, Issue 4, 2011, Pages 652-668

  Gadaleta, Agata ^a   Giancaspro, Angelica ^a   Cardone, Maria Francesca ^a   Blanco, Antonio ^a  

^a UNIVERSITY OF BARI   (Italy)

Author keywords

Biolistic method; Durum wheat; Glutenin subunits; HMW GS genes; Quantitative PCR assay; Real time PCR; Southern analysis; Transgene copy number; Transgene stable expression; Transgenic plants

Indexed keywords

GENOMIC DNA; GLUTENIN; PLANT DNA;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE DOSAGE; GENE EXPRESSION PROFILING; GENE IDENTIFICATION; GLU D1 1B GENE; GLU D1 2B GENE; HAPLOIDY; HOMOZYGOSITY; MOLECULAR PROBE; NONHUMAN; PLANT GENE; PLANT GENETICS; PLANT GENOME; PLASMID; PROTEIN SUBUNIT; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SOUTHERN BLOTTING; TRANSGENE; TRANSGENIC PLANT; TRITICUM DURUM; CALIBRATION; CHEMISTRY; GENETICS; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; WHEAT;

TRITICUM AESTIVUM; TRITICUM TURGIDUM SUBSP. DURUM;

BLOTTING, SOUTHERN; CALIBRATION; DNA, PLANT; GENE DOSAGE; GENOME, PLANT; IN SITU HYBRIDIZATION, FLUORESCENCE; PLANTS, GENETICALLY MODIFIED; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TRANSGENES; TRITICUM;

EID: 84855726095     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-011-0029-5     Document Type: Article

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