The human OCTN1 (SLC22A4) reconstituted in liposomes catalyzes acetylcholine transport which is defective in the mutant L503F associated to the Crohn's disease

Biochimica et Biophysica Acta - Biomembranes

Volumn 1818, Issue 3, 2012, Pages 559-565

  Pochini, Lorena ^a   Scalise, Mariafrancesca ^a   Galluccio, Michele ^a   Pani, Giovambattista ^b   Siminovitch, Katherine A ^c   Indiveri, Cesare ^a  

^a UNIVERSITY OF CALABRIA   (Italy)

^b CATHOLIC UNIVERSITY   (Italy)

^c MOUNT SINAI HOSPITAL   (Canada)

Author keywords

Acetylcholine; Crohn's disease; Liposome; OCTN1; Reconstitution; Transporter

Indexed keywords

ACETYLCARNITINE; ACETYLCHOLINE; ADENOSINE TRIPHOSPHATE; GAMMA BUTYROBETAINE; ORGANIC CATION TRANSPORTER 1; PROTEIN VARIANT; PROTEOLIPOSOME; SPERMIDINE; SPERMINE; TETRYLAMMONIUM;

ACETYLCHOLINE TRANSPORT; ARTICLE; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CROHN DISEASE; MUTANT; PRIORITY JOURNAL; TRANSPORT AT THE CELLULAR LEVEL; TRANSPORT KINETICS;

ACETYLCARNITINE; ACETYLCHOLINE; ADENOSINE TRIPHOSPHATE; AMINO ACID SUBSTITUTION; BETAINE; BIOLOGICAL TRANSPORT, ACTIVE; CARNITINE; CATALYSIS; CROHN DISEASE; HUMANS; KINETICS; LIPOSOMES; MUTATION, MISSENSE; NOOTROPIC AGENTS; ORGANIC CATION TRANSPORT PROTEINS; POTASSIUM CHANNEL BLOCKERS; TETRAETHYLAMMONIUM;

EID: 84855723434     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2011.12.014     Document Type: Article

References (39)

1. I. Tamai, R. Ohashi, J.I. Nezu, Y. Sai, D. Kobayashi, A. Oku, M. Shimane, and A. Tsuji Molecular and functional characterization of organic cation/carnitine transporter family in mice J. Biol. Chem. 275 2000 40064 40072
2. S.A. Eraly, J.C. Monte, and S.K. Nigam Novel slc22 transporter homologs in fly, worm, and human clarify the phylogeny of organic anion and cation transporters Physiol. Genomics 18 2004 12 24
3. H. Yabuuchi, I. Tamai, J. Nezu, K. Sakamoto, A. Oku, M. Shimane, Y. Sai, and A. Tsuji Novel membrane transporter OCTN1 mediates multispecific, bidirectional, and pH-dependent transport of organic cations J. Pharmacol. Exp. Ther. 289 1999 768 773
4. T. Nakamura, K. Yoshida, H. Yabuuchi, T. Maeda, and I. Tamai Functional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4) Biol. Pharm. Bull. 31 2008 1580 1584
5. D. Grundemann, S. Harlfinger, S. Golz, A. Geerts, A. Lazar, R. Berkels, N. Jung, A. Rubbert, and E. Schomig Discovery of the ergothioneine transporter Proc. Natl. Acad. Sci. U. S. A. 102 2005 5256 5261
6. A.M. Lamhonwah, and I. Tein Novel localization of OCTN1, an organic cation/carnitine transporter, to mammalian mitochondria Biochem. Biophys. Res. Commun. 345 2006 1315 1325
7. M. Galluccio, L. Pochini, L. Amelio, R. Accardi, M. Tommasino, and C. Indiveri Over-expression in E. coli and purification of the human OCTN1 transport protein Protein Expr. Purif. 68 2009 215 220
8. L. Pochini, M. Scalise, M. Galluccio, L. Amelio, and C. Indiveri Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli Biochem. J. 439 2011 227 233
9. I. Wessler, and C.J. Kirkpatrick Acetylcholine beyond neurons: the non-neuronal cholinergic system in humans Br. J. Pharmacol. 154 2008 1558 1571
10. O.R. Rana, P. Schauerte, R. Kluttig, J.W. Schroder, R.R. Koenen, C. Weber, K.W. Nolte, J. Weis, R. Hoffmann, N. Marx, and E. Saygili Acetylcholine as an age-dependent non-neuronal source in the heart Auton. Neurosci. 156 2010 82 89
11. S.M. Parsons Transport mechanisms in acetylcholine and monoamine storage FASEB J. 14 2000 2423 2434
12. K.S. Lips, C. Volk, B.M. Schmitt, U. Pfeil, P. Arndt, D. Miska, L. Ermert, W. Kummer, and H. Koepsell Polyspecific cation transporters mediate luminal release of acetylcholine from bronchial epithelium Am. J. Respir. Cell Mol. Biol. 33 2005 79 88
13. H. Koepsell, K. Lips, and C. Volk Polyspecific organic cation transporters: structure, function, physiological roles, and biopharmaceutical implications Pharm. Res. 24 2007 1227 1251
14. V.D. Peltekova, R.F. Wintle, L.A. Rubin, C.I. Amos, Q. Huang, X. Gu, B. Newman, M. Van Oene, D. Cescon, G. Greenberg, A.M. Griffiths, P.H. St George-Hyslop, and K.A. Siminovitch Functional variants of OCTN cation transporter genes are associated with Crohn disease Nat. Genet. 36 2004 471 475
15. R. Kramer, and C. Heberger Functional reconstitution of carrier proteins by removal of detergent with a hydrophobic ion exchange column Biochim. Biophys. Acta 863 1986 289 296
16. F. Palmieri, and M. Klingenberg Direct methods for measuring metabolite transport and distribution in mitochondria Methods Enzymol. 56 1979 279 301
17. C. Brizio, R. Brandsch, D. Bufano, L. Pochini, C. Indiveri, and M. Barile Over-expression in Escherichia coli, functional characterization and refolding of rat dimethylglycine dehydrogenase Protein Expr. Purif. 37 2004 434 442
18. I. Tamai, H. Yabuuchi, J. Nezu, Y. Sai, A. Oku, M. Shimane, and A. Tsuji Cloning and characterization of a novel human pH-dependent organic cation transporter, OCTN1 FEBS Lett. 419 1997 107 111
19. L. Pochini, F. Oppedisano, and C. Indiveri Reconstitution into liposomes and functional characterization of the carnitine transporter from renal cell plasma membrane Biochim. Biophys. Acta 1661 2004 78 86
20. F. Oppedisano, L. Pochini, M. Galluccio, M. Cavarelli, and C. Indiveri Reconstitution into liposomes of the glutamine/amino acid transporter from renal cell plasma membrane: functional characterization, kinetics and activation by nucleotides Biochim. Biophys. Acta 1667 2004 122 131
21. F. Oppedisano, and C. Indiveri Reconstitution into liposomes of the B°-like glutamine-neutral amino acid transporter from renal cell plasma membrane Biochim. Biophys. Acta 1778 2008 2258 2265
22. F. Palmieri, C. Indiveri, F. Bisaccia, and V. Iacobazzi Mitochondrial metabolite carrier proteins: purification, reconstitution, and transport studies Methods Enzymol. 260 1995 349 369
23. C. Indiveri Studying amino acid transport using liposomes Meth. Mol. Biol. 606 2010 55 68
24. M. Quick, and E.M. Wright Employing Escherichia coli to functionally express, purify, and characterize a human transporter Proc. Natl. Acad. Sci. U. S. A. 99 2002 8597 8601
25. T. Keller, D. Schwarz, F. Bernhard, V. Dotsch, C. Hunte, V. Gorboulev, and H. Koepsell Cell free expression and functional reconstitution of eukaryotic drug transporters Biochemistry 47 2008 4552 4564
26. S. Leviatan, K. Sawada, Y. Moriyama, and N. Nelson Combinatorial method for overexpression of membrane proteins in Escherichia coli J. Biol. Chem. 285 2010 23548 23556
27. T. Komatsu, M. Hiasa, T. Miyaji, T. Kanamoto, T. Matsumoto, M. Otsuka, Y. Moriyama, and H. Omote Characterization of the human MATE2 proton-coupled polyspecific organic cation exporter Int. J. Biochem. Cell Biol. 43 2011 913 918
28. A.M. Ojeda, N.G. Kolmakova, and S.M. Parsons Acetylcholine binding site in the vesicular acetylcholine transporter Biochemistry 43 2004 11163 11174
29. I. Wessler, H. Kilbinger, F. Bittinger, R. Unger, and C.J. Kirkpatrick The non-neuronal cholinergic system in humans: expression, function and pathophysiology Life Sci. 72 2003 2055 2061
30. G. Ciarimboli Role of organic cation transporters in drug-induced toxicity Expert Opin. Drug Metab. Toxicol. 7 2011 159 174
31. K. Kawashima, and T. Fujii Basic and clinical aspects of non-neuronal acetylcholine: overview of non-neuronal cholinergic systems and their biological significance J. Pharmacol. Sci. 106 2008 167 173
32. G. Bjelakovic, I. Stojanovic, T. Jevtovic Stoimenov, D. Pavlovic, G. Kocic, S. Rossi, C. Tabolacci, J. Nikolic, D. Sokolovic, and L. Bjelakovic Metabolic correlations of glucocorticoids and polyamines in inflammation and apoptosis Amino Acids 39 2010 29 43
33. R. Rodrigues Pereira, H.R. Scholte, I.E. Luyt-Houwen, and M.H. Vaandrager-Verduin Cardiomyopathy associated with carnitine loss in kidneys and small intestine Eur. J. Pediatr. 148 1988 193 197
34. P. Seth, X. Wu, W. Huang, F.H. Leibach, and V. Ganapathy Mutations in novel organic cation transporter (OCTN2), an organic cation/carnitine transporter, with differential effects on the organic cation transport function and the carnitine transport function J. Biol. Chem. 274 1999 33388 33392
35. Y. Wang, F. Taroni, B. Garavaglia, and N. Longo Functional analysis of mutations in the OCTN2 transporter causing primary carnitine deficiency: lack of genotype-phenotype correlation Hum. Mutat. 16 2000 401 407
36. S.A. Grando Biological functions of keratinocyte cholinergic receptors J. Investig. Dermatol. Symp. Proc. 2 1997 41 48
37. C.J. Rebouche, and H. Seim Carnitine metabolism and its regulation in microorganisms and mammals Annu. Rev. Nutr. 18 1998 39 61
38. P. Stenmark, D. Gurmu, and P. Nordlund Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism Biochemistry 43 2004 13996 14003
39. K.L. Guan, and Y. Xiong Regulation of intermediary metabolism by protein acetylation Trends Biochem. Sci. 36 2011 108 116