A novel strategy to over-express and purify homologous proteins from Streptococcus pneumoniae

Journal of Biotechnology

Volumn 157, Issue 2, 2012, Pages 279-286

  Lo Sapio, Morena ^a   Hilleringmann, Markus ^b   Barocchi, Michèle A ^a   Moschioni, Monica ^a  

^a NOVARTIS VACCINES AND DIAGNOSTICS   (Italy)

^b UNIVERSITY OF APPLIED SCIENCES   (Germany)

Author keywords

Homologous over expression; PMU1328; RrgB pilus protein purification; Streptococcus pneumoniae

Indexed keywords

BACTERIAL SPECIES; CHARACTERIZATION STUDIES; CLEAVAGE SITES; COMPLEMENTATION; HETEROLOGOUS EXPRESSION; HETEROLOGOUS SYSTEMS; HIS-TAG; HOMOLOGOUS PROTEINS; LEADER SEQUENCE; MASS SPECTROMETRY ANALYSIS; N-TERMINAL SEQUENCING; NOVEL STRATEGIES; OVER-EXPRESSION; PMU1328; PROOF OF PRINCIPLES; PROTEIN STRUCTURES; RRGB PILUS PROTEIN PURIFICATION; SECRETED PROTEIN; STREPTOCOCCUS PNEUMONIAE; SUPERNATANTS; TRYPSIN DIGESTION; VIRULENCE FACTORS; WILD TYPES;

ESCHERICHIA COLI; MASS SPECTROMETRY; PURIFICATION;

PROTEINS;

BACTERIAL PROTEIN; ESCHERICHIA COLI SECRETED PROTEIN A; POLYHISTIDINE TAG; TRYPSIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REPLICON; STREPTOCOCCUS PNEUMONIAE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS, GENETIC; REPLICON; SEQUENCE DELETION; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOCOCCUS PNEUMONIAE;

EID: 84855705031     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.11.011     Document Type: Article

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