Engineered Thermobifida fusca cutinase with increased activity on polyester substrates

Biotechnology Journal

Volumn 6, Issue 10, 2011, Pages 1230-1239

  Silva, Carla ^a   Da, Shi ^a   Silva, Nádia ^a   Matamá, Teresa ^a   Araújo, Rita ^a   Martins, Madalena ^a   Chen, Sheng ^b   Chen, Jian ^b   Wu, Jing ^b   Casal, Margarida ^a   Cavaco Paulo, Artur ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b JIANGNAN UNIVERSITY   (China)

Author keywords

Genetic modification; Hydrolysis; Mutant; Poly(ethylene terephthalate); Textiles

Indexed keywords

ACTIVE SITE; CATALYTIC SITES; CUTINASE; DOUBLE MUTANTS; DOUBLE MUTATION; GENETIC MODIFICATIONS; GENETICALLY MODIFIED; HYDROPHOBIC SUBSTRATE; MOLECULAR ENGINEERING; MUTANT; POLYESTER SURFACE; SITE DIRECTED MUTAGENESIS; SOLUBLE SUBSTRATES; THERMOBIFIDA FUSCA; WILD TYPES;

AMINO ACIDS; ESTERS; ETHYLENE; GENETIC ENGINEERING; HYDROLYSIS; HYDROPHOBICITY; POLYETHYLENE TEREPHTHALATES; SYNTHETIC FIBERS;

SUBSTRATES;

4 NITROPHENYLBUTYRIC ACID; AMINO ACID; BUTYRIC ACID DERIVATIVE; CUTINASE; ESTERASE; MUTANT PROTEIN; POLYESTER; POLYETHYLENE TEREPHTHALATE; TRIOLEIN; UNCLASSIFIED DRUG; WATER;

ACTINOBACTERIA; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTACT ANGLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME STABILITY; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; GENETIC ENGINEERING; HYDROLYSIS; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SITE DIRECTED MUTAGENESIS; STRUCTURAL HOMOLOGY; SURFACE PROPERTY; THERMOBIFIDA FUSCA; WETTABILITY; WILD TYPE;

ACTINOMYCETALES; BACTERIAL PROTEINS; CARBOXYLIC ESTER HYDROLASES; CATALYTIC DOMAIN; ESTERASES; HYDROLYSIS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; LIPASE; MICROSCOPY, ELECTRON, SCANNING; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POLYETHYLENE GLYCOLS; SUBSTRATE SPECIFICITY; TEXTILES; WETTABILITY;

BACTERIA (MICROORGANISMS); THERMOBIFIDA FUSCA;

EID: 80053929961     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000391     Document Type: Article

References (37)

1. Kim, H., Song, W., Lipase treatment of polyester fabrics. Fibers Polymers 2006, 7, 339-343.
2. Brueckner, T., Eberl, A., Heumann, S., Rabe, M., Guebitz, G. M., Enzymatic and chemical hydrolysis of poly(ethylene terephthalate) fabrics. J. Polymer Sci. A Polymer Chem. 2008, 46, 6435-6443.
3. Donelli, I., Taddei, P., Smet, P. F., Poelman, D. et al., Enzymatic surface modification and functionalization of PET: A water contact angle, FTIR, and fluorescence spectroscopy study. Biotechnol. Bioeng. 2009, 103, 845-856.
4. Pang, K., Kotek, R., Tonelli, A., Review of conventional and novel polymerization processes for polyesters. Prog. Polymer Sci. 2006, 31, 1009-1037.
5. Wang, P., Wang, Q., Fan, X., Cui, L. et al., Effects of cutinase on the enzymatic shrink-resist finishing of wool fabrics. Enzyme Microb. Technol. 2009, 44, 302-308.
6. Silva, C. M., Carneiro, F., O'Neill, A., Fonseca, L. P. et al., Cutinase-A new tool for biomodification of synthetic fibers. J. Polymer Sci. A Polymer Chem. 2005, 43, 2448-2450.
7. Alisch, M., Feuerhack, A., Müller, H., Mensak, B. et al., Biocatalytic modification of polyethylene terephthalate fibres by esterases from actinomycete isolates. Biocatal. Biotransform. 2004, 22, 347-351.
8. Hsieh, Y., Cram, L., Enzymatic hydrolysis to improve wetting and absorbency of polyester fabrics Textile Res. J. 1996, 68, 311-319.
9. Hsieh, Y., Hartzell, M., Boston, M., Clarkson, K. et al., US Patent 2003, 20030119172.
10. Yoon, M., Kellis, J., Poulouse, A., Enzymatic modification of polyester. AATCC Rev. 2002, 2, 33-36.
11. Miettinen-Oinonen, A., Puolakka, A., Buchert, J., Modification of textile fibres by laccase. Cost Action 847 Workshop 2004, 29-30.
12. Alisch-Mark, M., Herrmann, A., Zimmermann, W., Increase of the hydrophilicity of polyethylene terephthalate fibres by hydrolases from Thermomonospora fusca and Fusarium solani f. sp. Biotechnol. Lett. 2006, 28, 681-685.
13. O'Neill, A., Cavaco-Paulo, A., Monitoring biotransformations in polyesters. Biocatal. Biotransform. 2004, 22, 353-356.
14. Müller, R.-J., Schrader, H., Profe, J., Dresler, K., Deckwer, W.-D., Enzymatic degradation of poly(ethylene terephthalate): Rapid hydrolysis using a hydrolase from T. fusca. Macromol. Rapid Commun. 2005, 26, 1400-1405.
15. Silva, C., Cavaco-Paulo, A., Biotransformations in synthetic fibres. Biocatal. Biotransform. 2008, 26, 350-356.
16. Vertommen, M. A. M. E., Nierstrasz, V. A., Veer, M. v. d., Warmoeskerken, M. M. C. G., Enzymatic surface modification of poly(ethylene terephthalate). J. Biotechnol. 2005, 120, 376-386.
17. Nimchua, T., Punnapayak, H., Zimmermann, W., Comparison of the hydrolysis of polyethylene terephthalate fibers by a hydrolase from Fusarium oxysporum LCH I and Fusarium solani f. sp. pisi. Biotechnol. J. 2007, 2, 361-364.
18. Kontkanen, H., Saloheimo, M., Pere, J., Miettinen-Oinonen, A., Reinikainen, T., Characterization of Melanocarpus albomyces steryl esterase produced in Trichoderma reesei and modification of fibre products with the enzyme. Appl. Microbiol. Biotechnol. 2006, 72, 696-704.
19. Wang, X., Lu, D., Jonsson, L., Hong, F., Preparation of a PET-hydrolyzing lipase from Aspergillus oryzae by the addition of bis(2-hydroxyethyl) terephthalate to the culture medium and enzymatic modification of PET fabrics. Eng. Life Sci. 2008, 8, 268-276.
20. Araújo, R., Silva, C., O'Neill, A., Micaelo, N. et al., Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6, 6 fibers. J. Biotechnol. 2007, 128, 849-857.
21. Feuerhack, A., Alisch-Mark, M., Kisner, A., Pezzin, S. H. et al., Biocatalytic surface modification of knitted fabrics made of poly (ethylene terephthalate) with hydrolytic enzymes from Thermobifida fusca KW3b. Biocatal. Biotransform. 2008, 26, 357-364.
22. Liebminger, S., Eberl, A., Sousa, F., Heumann, S. et al., Hydrolysis of PET and bis-(benzoyloxyethyl) terephthalate with a new polyesterase from Penicillium citrinum. Biocatal. Biotransform. 2007, 25, 171-177.
23. Liu, Y., Wu, G., Gu, L., Enzymatic treatment of PET fabrics for improved hydrophilicity. AATCC Rev. 2008, 8, 44-48.
24. Almansa, E., Heumann, S., Eberl, A., Fischer-Colbrie, G. et al., Enzymatic surface hydrolysis of PET enhances bonding in PVC coating. Biocatal. Biotransform. 2008, 26, 365-370.
25. Dutta, K., Sen, S., Veeranki, V. D., Production, characterization and applications of microbial cutinases. Process Biochem. 2009, 44, 127-134.
26. Carvalho, C., Aires-Barros, M., Cabral, J. M. S., Cutinase structure, function and biocatalytic applications. Electron. J. Biotechnol. 1998, 1, 160-173.
27. Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., Cutinase: From molecular level to bioprocess development. Biotechnol. Bioeng. 1999, 66, 17-34.
28. Eberl, A., Heumann, S., Brückner, T., Araujo, R. et al., Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules. J. Biotechnol. 2009, 143, 207-212.
29. Gouda, M. K., Kleeberg, I., van den Heuvel, J., Müller, R.-J., Deckwer, W.-D., Production of a polyester degrading extracellular hydrolase from Thermomonospora fusca. Biotechnol. Prog. 2002, 18, 927-934.
30. Ronkvist, A. S. M., Xie, W., Lu, W., Gross, R. A., Cutinase-catalyzed hydrolysis of poly(ethylene terephthalate). Macromolecules 2009, 42, 5128-5138.
31. Chen, S., Tong, X., Woodard, R., Guochen, D. et al., Identification and characterization of bacterial cutinase. J. Biol. Chem. 2008, 283, 25854-25862.
32. He, G., Huo, G., Liu, L., Zhu, Y. et al., Enhanced cutinase production of Thermobifida fusca by a two-stage batch and fed-batch cultivation strategy. Biotechnol. Bioprocess Eng. 2009, 14, 46-51.
33. Kleeberg, I., Welzel, K., VandenHeuvel, J., Müller, R. J., Deckwer, W. D., Characterization of a new extracellular hydrolase from Thermobifida fusca degrading aliphatic aromatic copolyesters. Biomacromolecules 2004, 6, 262-270.
34. Eberl, A., Heumann, S., Kotek, R., Kaufmann, F. et al., Enzymatic hydrolysis of PTT polymers and oligomers. J. Biotechnol. 2008, 135, 45-51.
35. Chen, S., Su, L., Billig, S., Zimmermann, W. et al., Biochemical characterization of the cutinases from Thermobifida fusca. J. Mol. Catal. B Enzymatic 2010, 63, 121-127.
36. Fett, W. H. G., Moreau, R., Osman, S., Jones, L., Cutinase production by Streptomyces spp. Curr. Microbiol. 1992, 25, 165-171.
37. Jaeger, K., Ransac, S., Dijkstra, B., Colson, C. et al., Bacterial lipases FEMS Microbiol. Rev. 1994, 15, 29-63.