Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae

Applied Microbiology and Biotechnology

Volumn 67, Issue 6, 2005, Pages 778-788

  Maeda, Hiroshi ^a,b   Yamagata, Youhei ^b   Abe, Keietsu ^b   Hasegawa, Fumihiko ^b   Machida, Masayuki ^c   Ishioka, Ryoji ^d   Gomi, Katsuya ^b   Nakajima, Tasuku ^b  

^a Tohoku Technoarch   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^d Showa Highpolymer   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOLS; BIODEGRADATION; CARBOXYLIC ACIDS; EMULSIFICATION; FERMENTATION; FUNGI; ORGANIC POLYMERS; PLASTICS;

AMINO ACID SEQUENCES; FERMENTATION SUBSTRATES; IODEGRADABLE PLASTICS; SUPERNANTS;

ENZYMES;

ADIPIC ACID DERIVATIVE; ALCOHOL; BUTYRIC ACID; CARBON; CARBOXYLIC ACID; CUTINASE; FUNGAL ENZYME; HEXANOL; PLASTIC; POLYBUTYLENE SUCCINATE; POLYLACTIC ACID; POLYMER; SUCCINIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

BIODEGRADATION;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS ORYZAE; BINDING AFFINITY; BIODEGRADABILITY; CONTROLLED STUDY; EMULSION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; FERMENTATION TECHNIQUE; FUNGUS CULTURE; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; SUPERNATANT;

ADIPIC ACIDS; ASPERGILLUS ORYZAE; BIODEGRADATION, ENVIRONMENTAL; BUTYLENE GLYCOLS; BUTYRIC ACID; CARBOXYLIC ESTER HYDROLASES; CULTURE MEDIA; EMULSIONS; HEXANOLS; LACTIC ACID; MOLECULAR WEIGHT; PLASTICS; POLYMERS; SUBSTRATE SPECIFICITY; SUCCINATES;

ASPERGILLUS ORYZAE; FUNGI;

EID: 21344469738     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1853-6     Document Type: Article

References (56)

1. Ando Y, Yoshikawa K, Yoshikawa T, Nishioka M, Ishioka R, Yakabe Y (1998) Biodegradability of poly (tetramethylene succinate-co-tetramethylene adipate). I. Enzymatic hydrolysis. Polym Degrad Stabil 61:129-137
2. Barbesgaard P, Heldt-Hansen HP, Diderichsen B (1992) On the safety of Aspergillus oryzae: a review. Appl Microbiol Biotechnol 36:569-572
3. Christensen T, Woeldike H, Boel E, Mortensen SB, Hjortshoejk K, Thim L, Hansen MT (1988) High-level expression of recombinant genes in Aspergillus oryzae. Biotechnology 6:1419-1422
4. Cleland WW (1979) Substrate inhibition. Methods Enzymol 63:500-513
5. Coligan JE, Krusbeek AM, Margulies DH, Shevach EM, Strober W (1991) Current protocols in immunology. Greene/Wiley, New York
6. Egmond MR, de Vlieg J (2000) Fusarium solani pisi cutinase. Biochimie 82:1015-1021
7. Ferreira BS, Calado CR, van Keulen F, Fonseca LP, Cabral JM, da Fonseca MM (2003) Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects. Appl Microbiol Biotechnol 61:69-76
8. Fromm HJ (1979) Use of competitive inhibitors to study substrate binding order. Methods Enzymol 63:467-486
9. Fujimaki T (1998) Processability and properties of aliphatic polyesters, 'BIONOLLE', synthesized by polycondensation reaction. Polym Degrad Stabil 59:209-214
10. Gouka RJ, Punt PJ, van den Hondel CA (1997) Efficient production of secreted proteins by Aspergillus: progress, limitations and prospects. Appl Microbiol Biotechnol 47:1-11
11. Hatamoto O, Sekine H, Nakano E, Abe K (1999) Cloning and expression of a cDNA encoding the laccase from Schizophyllum commune. Biosci Biotechnol Biochem 63:58-64
12. Hawthorne BT, Rees-George J, Crowhurst RN (2001) Induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, Fusarium solani fsp. cucurbitae races one and two (Nectria haematococca MPI and MPV, respectively). FEMS Microbiol Lett 194:135-141
13. Hirano H, Watanabe T (1990) Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein. Electrophoresis 11:573-580
14. Huang SJ (1995) Polymer waste management-biodegradation, incineration, and recycling. J Macromol Sci Pure A 32:593-597
15. Ishigaki T, Sugano W, Ike M, Kawagoshi Y, Fukunaga I, Fujita M (2000) Abundance of polymers degrading microorganisms in a sea based solid waste disposal site. J Basic Microbiol 40:177-186
16. Kitakuni E, Yoshikawa K, Nakano K, Sasuga J, Nobiki M, Naoi H, Yokota Y, Ishioka R, Yakabe Y (2001) Biodegradation of poly (tetramethylene succinate-co-tetramethylene adipate) and poly (tetramethylene succinate) through water-soluble products. Environ Toxicol Chem 20:941-946
17. Kleeberg I, Hetz C, Kroppenstedt RM, Muller RJ, Deckwer WD (1998) Biodegradation of aliphatic-aromatic copolyesters by Thermomonospora fusca and other thermophilic compost isolates. Appl Environ Microbiol 64:1731-1735
18. Kolattukudy PE (1984) Detection of an N-terminal glucuronamide linkage in proteins. Methods Enzymol 106:210-217
19. Kolattukudy PE, Rogers LM, Li D, Hwang CS, Flaishman MA (1995) Surface signaling in pathogenesis. Proc Natl Acad Sci USA 92:4080-4087
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
21. Lin TS, Kolattukudy PE (1977) Glucuronyl glycine, a novel N-terminus in a glycoprotein. Biochem Biophys Res Commun 75:87-93
22. Lin TS, Kolattukudy PE (1980) Structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the N terminus. Eur J Biochem 106:341-351
23. Longhi S, Czjzek M, Lamzin V, Nicolas A, Cambillau C (1997a) Atomic resolution (1.0 A) crystal structure of Fusarium solani cutinase: stereochemical analysis. J Mol Biol 268:779-799
24. Longhi S, Mannesse M, Verheij HM, De Haas GH, Egmond M, Knoops-Mouthuy E, Cambillau C (1997b) Crystal structure of cutinase covalently inhibited by a triglyceride analogue. Protein Sci 6:275-286
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
26. Machida M (2002) Progress of Aspergillus oryzae genomics. Adv Appl Microbiol 51:81-106
27. Maeda H, Sano M, Maruyama Y, Tanno T, Akao T, Totsuka Y, Endo M, Sakurada R, Yamagata Y, Machida M, Akita O, Hasegawa F, Abe K, Gomi K, Nakajima T, Iguchi Y (2004) Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags. Appl Microbiol Biotechnol 65:74-83
28. Mannesse ML, Cox RC, Koops BC, Verheij HM, de Haas GH, Egmond MR, van der Hijden HT, de Vlieg J (1995) Cutinase from Fusarium solani pisi hydrolyzing triglyceride analogues. Effect of acyl chain length and position in the substrate molecule on activity and enantioselectivity. Biochemistry 34:6400-6407
29. Martinez C, De Geus P, Lauwereys M, Matthyssens G, Cambillau C (1992) Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent. Nature 356:615-618
30. Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035-10038
31. Mayer JM, Kaplin DL (1994) Biodegradable materials: balancing degradability and performance. Trends Polym Sci 2:227-235
32. Murphy CA, Cameron JA, Huang SJ, Vinopal RT (1996) Fusarium polycaprolactone depolymerase is cutinase. Appl Environ Microbiol 62:456-460
33. Nakamura K, Tomita T, Abe N, Kamio Y (2001) Purification and characterization of an extracellular poly(L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K104-1. Appl Environ Microbiol 67:345-353
34. Ohnishi K, Yoshida Y, Sekiguchi J (1994) Lipase production of Aspergillus oryzae. J Ferment Bioeng 77:490-495
35. Ohnishi K, Yoshida Y, Toida J, Sekiguchi J (1994) Purification and characterization of a novel lipolytic enzyme from Aspergillus oryzae. J Ferment Bioeng 78:413-419
36. Ohnishi K, Toida J, Nakazawa H, Sekiguchi J (1995) Genome structure and nucleotide sequence of a lipolytic enzyme gene of Aspergillus oryzae. FEMS Microbiol Lett 126:145-150
37. Potts J, Clendinning R, Ackart WB, Niegisch WD (1973) The biodegradability of synthetic polymers. In: Guillet J (eds) Polymers and ecological problems. Plenum, New York, pp 61-79
38. Prompers JJ, van Noorloos B, Mannesse ML, Groenewegen A, Egmond MR, Verheij HM, Hilbers CW, Pepermans HA (1999) NMR studies of Fusarium solani pisi cutinase in complex with phosphonate inhibitors. Biochemistry 38:5982-5994
39. Purdy RE, Kolattukudy PE (1975a) Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi. Biochemistry 14:2832-2840
40. Purdy RE, Kolattukudy PE (1975b) Hydrolysis of plant cuticle by plant pathogens. Purification, amino acid composition, and molecular weight of two isozymes of cutinase and a nonspecific esterase from Fusarium solani f. pisi. Biochemistry 14:2824-2831
41. Rudolph FB (1979) Product inhibition and abortive complex formation. Methods Enzymol 63:411-436
42. Rudolph FB, Fromm HJ (1979) Plotting methods for analyzing enzyme rate data. Methods Enzymol 63:138-159
43. Saito K, Miura N, Yamazaki M, Hirano H, Murakoshi I (1992) Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase. Proc Natl Acad Sci USA 89:8078-8082
44. Schafer W (1993) The role of cutinase in fungal pathogenicity. Trends Microbiol 1:69-71
45. Schagger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368-379
46. Shibuya I, Tsuchiya K, Tamura G, Ishikawa T, Hara S (1992) Overproduction of an alpha-amylase/glucoamylase fusion protein in Aspergillus oryzae using a high expression vector. Biosci Biotechnol Biochem 56:1674-1675
47. Suyama T, Hosoya H, Tokiwa Y (1998a) Bacterial isolates degrading aliphatic polycarbonates. FEMS Microbiol Lett 161:255-261
48. Suyama T, Tokiwa Y, Ouichanpagdee P, Kanagawa T, Kamagata Y (1998b) Phylogenetic affiliation of soil bacteria that degrade aliphatic polyesters available commercially as biodegradable plastics. Appl Environ Microbiol 64:5008-5011
49. Sweigard JA, Chumley FG, Valent B (1992a) Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea. Mol Gen Genet 232:174-182
50. Sweigard JA, Chumley FG, Valent B (1992b) Disruption of a Magnaporthe grisea cutinase gene. Mol Gen Genet 232:183-190
51. Takahashi T, Chang PK, Matsushima K, Yu J, Abe K, Bhatnagar D, Cleveland TE, Koyama Y (2002) Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR gene. Appl Environ Microbiol 68:3737-3743
52. Teeraphatpornchai T, Nakajima-Kambe T, Shigeno-Akutsu Y, Nakayama M, Nomura N, Nakahara T, Uchiyama H (2003) Isolation and characterization of a bacterium that degrades various polyester-based biodegradable plastics. Biotechnol Lett 25:23-28
53. Tsuchiya K, Nagashima T, Yamamoto Y, Gomi K, Kitamoto K, Kumagai C, Tamura G (1994) High level secretion of calf chymosin using a glucoamylase-prochymosin fusion gene in Aspergillus oryzae. Biosci Biotechnol Biochem 58:895-899
54. Tsuchiya K, Tada S, Gomi K, Kitamoto K, Kumagai C, Jigami Y, Tamura G (1992) High level expression of the synthetic human lysozyme gene in Aspergillus oryzae. Appl Microbiol Biotechnol 38:109-114
55. Uchida H, Nakajima-Kambe T, Shigeno-Akutsu Y, Nomura N, Tokiwa Y, Nakahara T (2000) Properties of a bacterium which degrades solid poly (tetramethylene succinate)-co-adipate, a biodegradable plastic. FEMS Microbiol Lett 189:25-29
56. Yu J, Proctor RH, Brown DW, Abe K, Gomi K, Machida M, Hasegawa F, Nierman WC, Bhatnagar D, Cleveland TE (2004). Genomics of economically significant Aspergillus and Fusarium species. In: Arora KD, Khachatourians GG (eds) Applied mycology and biotechnology, vol 4. Fungal genomics. Elsevier, Amsterdam