Isolation and identification of lactic acid bacteria from Tarag in Eastern Inner Mongolia of China by 16S rRNA sequences and DGGE analysis

Microbiological Research

Volumn 167, Issue 2, 2012, Pages 110-115

  Liu, Wenjun ^a,b   Bao, Qiuhua ^a,b   Jirimutu, ^a,b   Qing, Manjun ^a,b   Siriguleng, ^a,b   Chen, Xia ^a,b   Sun, Ting ^a,b   Li, Meihua ^a,b   Zhang, Jiachao ^a,b   Yu, Jie ^a,b   Bilige, Menghe ^a,b   Sun, Tiansong ^a,b   Zhang, Heping ^a,b  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

16S rRNA gene; Biodiversity; DGGE; Lactic acid bacteria; Tarag

Indexed keywords

16S RRNA GENE; 16S RRNA GENE SEQUENCE; 16S RRNA SEQUENCES; DGGE; FERMENTED DAIRY PRODUCTS; INNER MONGOLIA; ISOLATION AND IDENTIFICATION; LACTIC ACID BACTERIA; LACTOBACILLUS CASEI; LACTOBACILLUS HELVETICUS; LACTOCOCCUS LACTIS; MICROFLORA; MONGOLIA; PCR-DGGE; PROBIOTICS; PURE CULTURE; TARAG;

BIODIVERSITY; DAIRY PRODUCTS; GENES; LACTIC ACID; POLYMERASE CHAIN REACTION; RNA;

BACTERIA;

BACTERIAL DNA; RIBOSOME DNA; RNA 16S;

BACTERIUM; DAIRY FARMING; ELECTROKINESIS; FERMENTATION; IDENTIFICATION KEY; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; PROBIOTICS; SAMPLING; SPECIES DIVERSITY; SUBSPECIES;

ARTICLE; CHEMISTRY; CHINA; CLASSIFICATION; CLUSTER ANALYSIS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA FINGERPRINTING; DNA SEQUENCE; FOOD CONTROL; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LACTOBACILLALES; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY;

CHINA; CLUSTER ANALYSIS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA FINGERPRINTING; DNA, BACTERIAL; DNA, RIBOSOMAL; FOOD MICROBIOLOGY; HUMANS; LACTOBACILLALES; MOLECULAR SEQUENCE DATA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

CHINA; NEI MONGGOL;

BACTERIA (MICROORGANISMS); LACTOBACILLUS CASEI; LACTOBACILLUS HELVETICUS; LACTOCOCCUS LACTIS; LACTOCOCCUS LACTIS SUBSP. LACTIS;

EID: 84855486643     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2011.05.001     Document Type: Article

References (23)

1. An Y., Adachi Y., Ogawa Y. Classification of lactic acid bacteria isolated from chigee and mare milk collected in Inner Mongolia. Animal Science Journal 2004, 75:245-252.
2. Bao Y., Zhang Y., Zhang Y., Liu Y., Wang S., Dong X., Wang Y., Zhang H. Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control 2010, 21:695-701.
3. Chen Y., Wang Z., Chen X., Liu Y., Zhang H., Sun T. Identification of angiotensin I-converting enzyme inhibitory peptides from koumiss, a traditional fermented mare's milk. Journal of Dairy Science 2010, 93(3):884-892.
4. Han X., Owens K., Wu X.B., Wu J., Huang J. The grasslands of Inner Mongolia: a special feature. Rangeland Ecology and Management 2009, 62:303-304.
5. Kang L., Han X., Zhang Z., Sun O.J. Grassland ecosystems in China: review of current knowledge and research advancement. Philosophical Transactions of the Royal Society B: Biological Sciences 2007, 362:997-1008.
6. Liu W., Sun Z., Zhang J., Gao W., Wang W., Wu L., Sun T., Chen W., Liu X., Zhang H. Analysis of microbial composition in acid whey for dairy fan making in Yunnan by conventional method and 16S rRNA sequencing. Current Microbiology 2009, 59:199-205.
7. Ma J., Kong J., Ji M. Detection of the lactic acid bacteria in commercial yoghurts by PCR-denaturing gradient gel electrophoresis. Chinese Journal of Applied Environmental Biology 2009, 15(4):534-539.
8. Muyzer G., de Waal E.C., Uitterlinden A.G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 1993, 59:695-700.
9. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology Evolution 1987, 4:406-425.
10. Scarpellini M., Mora D., Colombo S., Franzetti L. Development of genus/species-specific PCR analysis for identification of Carnobacterium strains. Current Microbiology 2002, 45:24-29.
11. Shuangquan, Burentegusi, Yu B., Miyamoto T. Microflora in traditional starter cultures for fermented milk, hurunge, from Inner Mongolia, China. Animal Science Journal 2006, 77:235-241.
12. Sun Z.H., Liu W.J., Zhang J.C., Yu J., Gao W., Jiri M., Menghe B., Sun T.S., Zhang H.P. Identification and characterization of the dominant lactic acid bacteria isolated from traditional fermented milk in Mongolia. Folia Microbiology 2010, 55(3):270-276.
13. Sun Z., Liu W., Gao W., Yang M., Zhang J., Wu L., Wang J., Menghe B., Sun T., Zhang H. Identification and characterization of the dominant lactic acid bacteria from kurut: the naturally fermented yak milk in Qinghai, China. Journal of General and Applied Microbiology 2010, 56:1-10.
14. Tamine A.Y., Robinson R.K. Fermented milks and their future trends: technological aspects. Journal of Dairy Research 1988, 55:281-307.
15. Tuo Y., Zhang Q., Chu F., Merritt J., Menghe B., Sun T., Du R., Zhang H. Immunological evaluation of Lactobacillus casei Zhang: a newly isolated strain from koumiss in Inner Mongolia, China. BMC Immunology 2008, 9:68.
16. Uchida K., Hirata M., Motoshima H., Urashima T., Arai I. Microbiota of 'Airag', 'Tarag' and other kinds of fermented dairy products from nomad in Mongolia. Animal Science Journal 2007, 78:650-658.
17. Vinderola C.G., Bailo N., Reinheimer J.A. Survival of probiotic microflora in Argentinean yoghurts during refrigerated storage. Food Research International 2000, 33:97-102.
18. Wang J., Chen X., Liu W., Yang M., Airidengcaicike, Zhang H. Identification of lactobacillus from koumiss by conventional and molecular methods. European Food Research and Technology 2008, 227:1555-1561.
19. Watanabe K., Fujimoto J., Sasamoto M., Dugersuren J., Tumursuh T., Demberel S. Diversity of lactic acid bacteria and yeasts in Airag and Tarag, traditional fermented milk products of Mongolia. World Journal of Microbiology and Biotechnology 2008, 24:1313-1325.
20. Wu R., Wang W., Yu D., Zhang W., Li Y., Sun Z., Wu J., Meng H., Zhang H. Proteomic analysis of Lactobacillus casei Zhang, a new probiotic bacterium isolated from traditionally home-made Koumiss in Inner Mongolia of China. Molecular and Cellular Proteomics 2009, 8:2321-2338.
21. Wu R., Wang L., Wang J., Li H., Menghe B., Wu J., Guo M., Zhang H. Isolation and preliminary probiotic selection of lactobacilli from koumiss in Inner Mongolia. Journal of Basic Microbiology 2009, 49:1-9.
22. Wu Z. Medical therapy of Koumiss 1986, Inner Mongolia People's Press, China, pp. 52-198.
23. Zhu H., Qu F., Zhu L.H. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Research 1993, 21:5279-5280.