Fascin, a novel marker of human hepatic stellate cells, may regulate their proliferation, migration, and collagen gene expression through the FAK-PI3K-Akt pathway

Laboratory Investigation

Volumn 92, Issue 1, 2012, Pages 57-71

  Uyama, Naoki ^a   Iimuro, Yuji ^a   Kawada, Norifumi ^b   Reynaert, Hendrik ^c   Suzumura, Kazuhiro ^a   Hirano, Tadamichi ^a   Kuroda, Nobukazu ^a   Fujimoto, Jiro ^a  

^a HYOGO COLLEGE OF MEDICINE   (Japan)

^b OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c UNIVERSITY HOSPITAL BRUSSELS   (Belgium)

Author keywords

actin bundling protein; fascin; hepatic stellate cells; liver; portal fibroblasts

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN TYPE 1; COLLAGEN TYPE 3; FASCIN; FIBULIN; FIBULIN 2; FOCAL ADHESION KINASE; GELATINASE A; MATRIGEL; MATRIX METALLOPROTEINASE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; VIMENTIN;

ADULT; AGED; ARTICLE; CELL ASSAY; CELL CULTURE; CELL FUNCTION; CELL LINE; CELL MIGRATION; CELL PROLIFERATION; CLINICAL ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DOWN REGULATION; FEMALE; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LIVER SINUSOID; MALE; MYOFIBROBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STELLATE CELL; WESTERN BLOTTING;

EID: 84855233177     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2011.150     Document Type: Article

References (46)

1. Wake K. Perisinusoidal stellate cells (fat-storing cells, interstitial cells, lipocytes), their related structure in and around the liver sinusoids, and vitamin A-storing cells in extrahepatic organs. Int Rev Cytol 1980;66:303-353. (Pubitemid 10028209)
2. Friedman SL. Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. N Engl J Med 1993;328:1828-1835. (Pubitemid 23174715)
3. Pinzani M. Novel insights into the biology and physiology of the Ito cell. Pharmacol Ther 1995;66:387-412.
4. Gressner AM. Cytokines and cellular crosstalk involved in the activation of fat-storing cells. J Hepatol 1995;22:28-36.
5. Ramadori G, Veit T, Schwogler S, et al. Expression of the gene of the alpha-smooth muscle-actin isoform in rat liver and in rat fat-storing (ITO) cells. Virchows Archiv 1990;59:349-357.
6. Iredale JP, Benyon RC, Arthur MJ, et al. Tissue inhibitor of metalloproteinase-1 messenger RNA expression is enhanced relative to interstitial collagenase messenger RNA in experimental liver injury and fibrosis. Hepatology (Baltimore, MD) 1996;24:176-184. (Pubitemid 26249431)
7. Theret N, Musso O, L'helgoualc'h A, et al. Activation of matrix metalloproteinase-2 from hepatic stellate cells requires interactions with hepatocytes. Am J Pathol 1997;150:51-58. (Pubitemid 27027095)
8. Ramadori G, Saile B. Portal tract fibrogenesis in the liver. Lab Invest J Tech Methods Pathol 2004;84:153-159. (Pubitemid 41656496)
9. Dubuisson L, Lepreux S, Bioulac-Sage P, et al. Expression and cellular localization of fibulin-1 in normal and pathological human liver. J Hepatol 2001;34:514-522. (Pubitemid 32328507)
10. Saile B, Matthes N, Neubauer K, et al. Rat liver myofibroblasts and hepatic stellate cells differ in CD95-mediated apoptosis and response to TNF-alpha. Am J Physiol 2002;283:G435-G444. (Pubitemid 34791311)
11. Knittel T, Kobold D, Saile B, et al. Rat liver myofibroblasts and hepatic stellate cells: different cell populations of the fibroblast lineage with fibrogenic potential. Gastroenterology 1999;117:1205-1221. (Pubitemid 29512681)
12. Kobold D, Grundmann A, Piscaglia F, et al. Expression of reelin in hepatic stellate cells and during hepatic tissue repair: a novel marker for the differentiation of HSC from other liver myofibroblasts. J Hepatol 2002;36:607-613. (Pubitemid 34499397)
13. Cassiman D, Libbrecht L, Desmet V, et al. Hepatic stellate cell/ myofibroblast subpopulations in fibrotic human and rat livers. J Hepatol 2002;36:200-209. (Pubitemid 34146426)
14. Dranoff JA, Kruglov EA, Robson SC, et al. The ecto-nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver. Hepatology (Baltimore, MD) 2002;36:1135-1144. (Pubitemid 35253429)
15. Nakatani K, Seki S, Kawada N, et al. Expression of neural cell adhesion molecule (N-CAM) in perisinusoidal stellate cells of the human liver. Cell Tissue Res 1996;283:159-165. (Pubitemid 26009286)
16. Adams JC. Formation of stable microspikes containing actin and the 55 kDa actin bundling protein, fascin, is a consequence of cell adhesion to thrombospondin-1: implications for the anti-adhesive activities of thrombospondin-1. J Cell Sci 1995;108(Part 5):1977-1990.
17. Yamashiro-Matsumura S, Matsumura F. Intracellular localization of the 55-kD actin-bundling protein in cultured cells: spatial relationships with actin, alpha-actinin, tropomyosin, and fimbrin. J Cell Biol 1986;103:631-640. (Pubitemid 16055056)
18. Mosialos G, Yamashiro S, Baughman RW, et al. Epstein-Barr virus infection induces expression in B lymphocytes of a novel gene encoding an evolutionarily conserved 55-kilodalton actin-bundling protein. J Virol 1994;68:7320-7328. (Pubitemid 24328786)
19. Adams JC. Roles of fascin in cell adhesion and motility. Curr Opin Cell Biol 2004;16:590-596. (Pubitemid 39201245)
20. Hashimoto Y, Skacel M, Adams JC. Roles of fascin in human carcinoma motility and signaling: prospects for a novel biomarker? Int J Biochem Cell Biol 2005;37:1787-1804. (Pubitemid 40961664)
21. Edwards RA, Bryan J. Fascins, a family of actin bundling proteins. Cell Motility Cytoskeleton 1995;32:1-9.
22. Adams JC. Characterization of cell-matrix adhesion requirements for the formation of fascin microspikes. Mol Biol Cell 1997;8:2345-2363. (Pubitemid 27469392)
23. Adams JC, Kureishy N, Taylor AL. A role for syndecan-1 in coupling fascin spike formation by thrombospondin-1. J Cell Biol 2001;152: 1169-1182. (Pubitemid 34280175)
24. Grothey A, Hashizume R, Ji H, et al. C-erbB-2/HER-2 upregulates fascin, an actin-bundling protein associated with cell motility, in human breast cancer cell lines. Oncogene 2000;19:4864-4875.
25. Grothey A, Hashizume R, Sahin AA, et al. Fascin, an actin-bundling protein associated with cell motility, is upregulated in hormone receptor negative breast cancer. Br J Cancer 2000;83:870-873.
26. Jawhari AU, Buda A, Jenkins M, et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro. Am J Pathol 2003;162:69-80. (Pubitemid 36041990)
27. Pelosi G, Pasini F, Sonzogni A, et al. Prognostic implications of neuroendocrine differentiation and hormone production in patients with stage I nonsmall cell lung carcinoma. Cancer 2003;97: 2487-2497. (Pubitemid 36542488)
28. Hashimoto Y, Skacel M, Lavery IC, et al. Prognostic significance of fascin expression in advanced colorectal cancer: an immunohistochemical study of colorectal adenomas and adenocarcinomas. BMC Cancer 2006;6:241.
29. Kabukcuoglu S, Ozalp SS, Oner U, et al. Actin bundling protein fascin expression in ovarian neoplasms: comparison of histopathologic features of tumors obtained by the first and secondary cytoreduction surgeries. Eur J Gynaecol Oncol 2006;27:123-128.
30. Zhang H, Xu L, Xiao D, et al. Fascin is a potential biomarker for earlystage oesophageal squamous cell carcinoma. J Clin Pathol 2006;59:958-964. (Pubitemid 44422851)
31. Zigeuner R, Droschl N, Tauber V, et al. Biologic significance of fascin expression in clear cell renal cell carcinoma: systematic analysis of primary and metastatic tumor tissues using a tissue microarray technique. Urology 2006;68:518-522. (Pubitemid 44436491)
32. Puppa G, Maisonneuve P, Sonzogni A, et al. Independent prognostic value of fascin immunoreactivity in stage III-IV colonic adenocarcinoma. Br J Cancer 2007;96:1118-1126. (Pubitemid 46608324)
33. Vignjevic D, Kojima S, Aratyn Y, et al. Role of fascin in filopodial protrusion. J Cell Biol 2006;174:863-875. (Pubitemid 44373735)
34. Xie JJ, Xu LY, Zhang HH, et al. Role of fascin in the proliferation and invasiveness of esophageal carcinoma cells. Biochem Biophys Res Commun 2005;337:355-362. (Pubitemid 41393802)
35. Bedossa P, Poynard T. An algorithm for the grading of activity in chronic hepatitis C. The MRTAVIR Cooperative Study Group. Hepatology (Baltimore, MD) 1996;24:289-293. (Pubitemid 26260021)
36. Xu L, Hui AY, Albanis E, et al. Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis. Gut 2005;54: 142-151. (Pubitemid 40007234)
37. PE Applied Biosystems. Relative quantitation of gene expression. ABI Prism 7700 sequence detection system: User Bulletin #2, 1997, pp 11-15.
38. De Arcangelis A, Georges-Labouesse E, Adams JC. Expression of fascin-1, the gene encoding the actin-bundling protein fascin-1, during mouse embryogenesis. Gene Expr Patterns 2004;4:637-643. (Pubitemid 39307527)
39. Herbst H, Frey A, Heinrichs O, et al. Heterogeneity of liver cells expressing procollagen types I and IV in vivo. Histochem Cell Biol 1997;107:399-409. (Pubitemid 27226837)
40. Kawada N, Kuroki T, Kobayashi K, et al. Expression of heat-shock protein 47 in mouse liver. Cell Tissue Res 1996;284:341-346. (Pubitemid 26140533)
41. Knittel T, Aurisch S, Neubauer K, et al. Cell-type-specific expression of neural cell adhesion molecule (N-CAM) in Ito cells of rat liver. Upregulation during in vitro activation and in hepatic tissue repair. Am J Pathol 1996;149:449-462. (Pubitemid 26256333)
42. Hayashi Y, Toda K, Saibara T, et al. Expression of fascin-1, an actinbundling protein, in migrating hepatoblasts during rat liver development. Cell Tissue Res 2008;334:219-226.
43. Giancotti FG, Ruoslahti E. Integrin signaling. Science (New York, NY) 1999;285:1028-1032.
44. Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol 2007;22(Suppl 1):S79-S84. (Pubitemid 46848998)
45. Reif S, Lang A, Lindquist JN, et al. The role of focal adhesion kinasephosphatidylinositol 3-kinase-akt signaling in hepatic stellate cell proliferation and type I collagen expression. J Biolog Chem 2003;278: 8083-8090. (Pubitemid 36800548)
46. Bae IH, Yoon SH, Lee SB, et al. Signaling components involved in Bcl-winduced migration of gastric cancer cells. Cancer Lett 2009;277:22-28.