Preservation of primer and probes on "ready-to-use" 96-well microtiter plates: A step forward towards enhancing throughput and harmonization of real-time PCR applications in food and feed control

Food Control

Volumn 25, Issue 2, 2012, Pages 709-716

  Zagon, Jutta ^a   Kurth, Sylvia ^a   Ehlers, Anke ^a   Linke, Bettina ^a   Lampen, Alfonso ^a   Broll, Hermann ^a  

^a FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

Author keywords

DNA; Long term storage; Microtiter plate; Pre coating; Real time PCR; Trehalose

Indexed keywords

EID: 84855175529     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2011.12.006     Document Type: Article

References (26)

1. Bonnet J., Colotte M., Coudy D., Couallier V., Portier J., Morin B., et al. Chain and conformation stability of solid-state DNA: implications for room temperature storage. Nucleic Acids Research 2010, 38(5):1531-1546.
2. Brezna B., Hudecova L., Kuchta T. Anovel real-time polymerase chain reaction (PCR) method for the detection of walnuts in food. European Food Research and Technology 2006, 223:373-377.
3. Bruskov V.I., Malakhova L.V., Masalimov Z.K., Chernikov A.V. Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA. Nucleic Acids Research 2002, 30(6):1354-1363.
4. Burke M.J. The glassy state and survival of anhydrous biological systems. Membranes, metabolism and dry organisms 1985, 358-363. Cornell University Press, Ithaca, New York. A.C. Leopold (Ed.).
5. BVL L 23.01.22-1. Technische Regel: Untersuchung von Lebensmitteln - Nachweis einer gentechnischen Veränderung von Sojabohnen durch Amplifizierung der veränderten DNA-Sequenz mit Hilfe der PCR (polymerase chain reaction) und Hybridisierung des PCR-Produktes mit einer DNA-Sonde. Ausgabe 1998-03. Berlin: Beuth Verlag.
6. Clegg J.S. The physical properties and metabolic status of Artemia cysts at low water contents: the "water replacement hypothesis" Membranes, metabolism and dry organisms 1985, 169-187. Cornell University Press, Ithaca, New York. A.C. Leopold (Ed.).
7. Directive 2000/13/EC of the European Parliament and the council of 20 March 2000 on the approximation of the laws of the member states relating to the labelling, presentation and advertising of foodstuffs. Official Journal of the European Communities 06.05.2000, L109:29.
8. Dixon D.R., Dixon L.R.J., Pascoe P.L., Wilson J.T. Chromosomal and nuclear characteristics of deep-sea hydrothermal-vent organisms: correlates of increased growth rate. Marine Biology 2001, 139:251-255.
9. Erfle H., Neumann B., Liebel U., Rogers P., Held M., Walter T., et al. Reverse transfection on cell arrays for high content screening microscopy. Nature Protocols 2007, 2:392-399.
10. Evans R.K., Xu Z., Bohannon K.E., Wang B., Bruner M.W., Volkin D.B. Evaluation of degradation pathways for plasmid DNA in pharmaceutical formulations via accelerated stability studies. Journal of Pharmacological Sciences 2000, 89:76-87.
11. Hemmerich K.J. General aging theory and simplified protocol for accelerated aging of medical devices. Medical Device Link 1998, Online publication (08.08.11). http://www.mddionline.com/article/general-aging-theory-and-simplified-protocol-accelerated-aging-medical-devices.
12. Karimata H., Nakano S., Ohmichi T., Kawakami J., Sugimoto N. Stabilization of a DNA duplex under molecular crowding conditions of PEG. Nucleic Acids Symposium Series 2004, 48:107-108.
13. Klatser P.R., Kuijper S., van Ingen C.W., Kolk A.H.J. Stabilized, freeze-dried PCR mix for detection of mycobacteria. Journal of Clinical Microbiology 1998, 36(6):1798-1800.
14. Köppel R., Zimmerli F., Breitenmoser A. Heptaplex real-time PCR for the identification and quantification of DNA from beef, pork, chicken, turkey, horse meat, sheep (mutton) and goat. European Food Research and Technology 2009, 230:125-133.
15. Kurz M. Compatible solute influence on nucleic acids: many questions but few answers. Saline Systems 2008, 4:6.
16. Laube I., Hird H., Brodmann P., Ullmann S., Schone-Michling M., Chisholm J., et al. Development of primer and probe sets for the detection of plant species in honey. Food Chemistry 2010, 118:979-986.
17. Lins R.D., Pereira C.S., Hünenberger P.H. Trehalose-protein interaction in aqueous solution. Proteins 2004, 55:177-186.
18. Mazzara M., Savini C., Delobel C., Broll H., Damant A., Paoletti C., et al. Definition of minimum performance requirements for analytical methods of GMO testing 2008, Office for Official Publications of the European Communities, Luxembourg, EUR 23696 EN. 10.2788/65827.
19. Pereira C.S., Lins R.D., Chandrasekhar I., Freitas L.C.G., Hünenberger P.H. Interaction of the disaccharide trehalose with a phospholipid bilayer: a molecular dynamics study. Biophysical Journal 2004, 86:2273-2285.
20. Quaak S.G.L., Haanen J.B.A.G., Beijnen J.H., Nuijen B. Naked plasmid DNA formulation: effect of different disaccharides on stability after lyophilisation. AAPS PharmSciTech 2010, 11(1):344-350.
21. Schnier P.D., Klassen J.S., Strittmatter E.F., Williams E.R. Activation energies for dissociation of double strand oligonucleotide anions: evidence for Watson-Crick base pairing in vacuo. Journal of the American Chemical Society 1998, 120(37):9605-9613.
22. Shirkey B., McMaster N.J., Smith S.C., Wright D.J., Rodriguez H., Jaruga P., et al. Genomic DNA of Nostoc commune (Cyanobacteria) becomes covalently modified during long-term (decades) desiccation but is protected from oxidative damage and degradation. Nucleic Acids Research 2003, 31(12):2995-3005.
23. Spiess A.N., Mueller N., Ivell R. Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose. Clinical Chemistry 2004, 50(7):1256-1259.
24. Teramoto N., Sachinvala N.D., Shibata M. Trehalose and trehalose-based polymers for environmentally benign, biocompatible and bioactive materials. Molecules 2008, 13:1773-1816.
25. Witek M.A., Llopis S.D., Wheatley A., McCarley R.L., Soper S.A. Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips. Nucleic Acids Research 2006, 34(10):e74.
26. Ziauddin J., Sabatini D.M. Microarrays of cells expressing defined cDNAs. Nature 2001, 411:107.