Site-specific protein cleavage in vivo by an intein-derived protease

FEBS Letters

Volumn 586, Issue 1, 2012, Pages 79-84

  Volkmann, Gerrit ^a,b   Volkmann, Verena ^a   Liu, Xiang Qin ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Protease; Protein cleavage; Site specific proteolysis; Split intein

Indexed keywords

INTEIN; PROTEINASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL VIABILITY; CONTROLLED STUDY; GENE DELETION; IN VIVO STUDY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN STRUCTURE; SYNECHOCYSTIS;

AMINO ACID SEQUENCE; CYSTEINE; CYTOPLASM; ESCHERICHIA COLI; INTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PEPTIDE HYDROLASES; PROTEINS; SACCHAROMYCES CEREVISIAE; SYNECHOCYSTIS;

BACTERIA (MICROORGANISMS); EUKARYOTA; SYNECHOCYSTIS SP.;

EID: 84655167625     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2011.11.028     Document Type: Article

References (14)

1. Y.P. Shih, H.C. Wu, S.M. Hu, T.F. Wang, and A.H. Wang Self-cleavage of fusion protein in vivo using TEV protease to yield native protein Protein Sci 14 2005 936 941 (Pubitemid 40389363)
2. B. Harder, A. Schomburg, R. Pflanz, K. Kustner, N. Gerlach, and R. Schuh TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms Biotechniques 44 2008 765 772 (Pubitemid 351839145)
3. D.C. Gray, S. Mahrus, and J.A. Wells Activation of specific apoptotic caspases with an engineered small-molecule-activated protease Cell 142 2010 637 646
4. M. Jungbluth, C. Renicke, and C. Taxis Targeted protein depletion in Saccharomyces cerevisiae by activation of a bidirectional degron BMC Syst Biol 4 2010 176
5. G. Kostallas, P.A. Lofdahl, and P. Samuelson Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay PLoS One 6 2011 e16136
6. T. Sonntag, and H.D. Mootz An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing Mol Biosyst 7 2011 2031 2039
7. M. Pogson, G. Georgiou, and B.L. Iverson Engineering next generation proteases Curr Opin Biotechnol 20 2009 390 397
8. W. Sun, J. Yang, and X.Q. Liu Synthetic two-piece and three-piece split inteins for protein trans-splicing J Biol Chem 279 2004 35281 35286 (Pubitemid 39100525)
9. G. Volkmann, W. Sun, and X.Q. Liu Controllable protein cleavages through intein fragment complementation Protein Sci 18 2009 2393 2402
10. J. Perez-Perez, and J. Gutierrez An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmids Gene 158 1995 141 142
11. C.J. Noren, J. Wang, and F.B. Perler Dissecting the chemistry of protein splicing and its applications Angew Chem Int Ed Engl 39 2000 450 466
12. G. Amitai, B.P. Callahan, M.J. Stanger, G. Belfort, and M. Belfort Modulation of intein activity by its neighboring extein substrates Proc Natl Acad Sci USA 106 2009 11005 11010
13. C. Ludwig, D. Schwarzer, and H.D. Mootz Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction J Biol Chem 283 2008 25264 25272
14. Y. Ding, M.Q. Xu, I. Ghosh, X. Chen, S. Ferrandon, G. Lesage, and Z. Rao Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing J Biol Chem 278 2003 39133 39142 (Pubitemid 37221816)