An improved non-enzymatic "dNA ladder assay" for more sensitive and early detection of apoptosis

Cytotechnology

Volumn 64, Issue 1, 2012, Pages 9-14

  Suman, Shubhankar ^a   Pandey, Akshay ^a   Chandna, Sudhir ^a  

^a INSTITUTE OF NUCLEAR MEDICINE AND ALLIED SCIENCES   (India)

Author keywords

Apoptosis; Cell death; DNA fragmentation; DNA ladder; Sf9

Indexed keywords

DIMETHYL SULFOXIDE; DNA; DNA FRAGMENT;

ANALYTIC METHOD; APOPTOSIS; ARTICLE; CELL ASSAY; CONTROLLED STUDY; DNA FRAGMENTATION; DNA LADDER ASSAY; SENSITIVITY ANALYSIS;

EID: 84555208625     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-011-9395-0     Document Type: Article

References (28)

1. T Arakawa Y Kita SN Timasheff 2007 Protein precipitation and denaturation by dimethyl sulfoxide Biophys Chem 131 62 70 10.1016/j.bpc.2007.09.004 1:CAS:528:DC%2BD2sXht1yltbbF (Pubitemid 350047519)
2. AG Basnakian SJ James 1994 A rapid and sensitive assay for detection of DNA fragmentation during early phases of apoptosis Nucleic Acids Res 22 2714 2715 10.1093/nar/22.13.2714 1:CAS:528:DyaK2cXlsV2qs7k%3D (Pubitemid 24222458)
3. S Chandna 2004 Single-cell gel electrophoresis assay monitors precise kinetics of DNA fragmentation induced during programmed cell death Cytometry Part A 61A 127 133 10.1002/cyto.a.20071 1:CAS:528:DC%2BD2cXhtVSht7fM (Pubitemid 39362287)
4. S Chandna BS Dwarakanath RK Seth D Khaitan JS Adhikari V Jain 2004 Radiation responses of Sf9, a highly radioresistant lepidopteran insect cell line Int J Radiat Biol 80 301 315 10.1080/09553000410001679794 1:CAS:528:DC%2BD2cXjvFaqt74%3D (Pubitemid 38736759)
5. MM Compton 1992 A biochemical hallmark of apoptosis: intranucleosomal degradation of the genome Cancer Metast Rev 11 105 119 10.1007/BF00048058 1:CAS:528:DyaK3sXmt1agsbk%3D
6. S Czene E Testa J Nygren I Belyaev MH Harms-Ringdahl 2002 DNA fragmentation and morphological changes in apoptotic human lymphocytes Biochem Biophys Res Commun 294 872 878 10.1016/S0006-291X(02)00588-0 1:CAS:528:DC%2BD38Xks1GnsLc%3D (Pubitemid 34687197)
7. L Di EH Kerns 2006 Biological assay challenges from compound solubility: strategies for bioassay optimization Drug Discov Today 11 446 451 10.1016/j.drudis.2006.03.004 1:CAS:528:DC%2BD28XjvVGns7g%3D
8. J Gong F Traganos Z Darzynkiewicz 1994 A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry Anal Biochem 218 314 319 10.1006/abio.1994.1184 1:CAS:528: DyaK2cXivFensrg%3D (Pubitemid 24141629)
9. BG Hermann AM Frischauf 1987 Isolation of genomic DNA Methods Enzymol (US) 152 180 183 10.1016/0076-6879(87)52018-3
10. J Kang MS Lee DG Gorenstein 2005 The enhancement of PCR amplification of a random sequence DNA library by DMSO and betaine: application to in vitro combinatorial selection of aptamers J Biochem Biophys Methods 64 147 151 10.1016/j.jbbm.2005.06.003 1:CAS:528:DC%2BD2MXos1Wrsbw%3D (Pubitemid 41169782)
11. JF Kerr AH Wyllie AR Currie 1972 Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics Br J Cancer 26 239 257 10.1038/bjc.1972.33 1:STN:280:DyaE3s%2FgsFSksw%3D%3D
12. R Kumarswamy RK Seth BS Dwarakanath S Chandna 2009 Mitochondrial regulation of insect cell apoptosis: evidence for permeability transition pore-independent cytochrome-c release in the Lepidopteran Sf9 cells Int J Biochem Cell Biol 41 1430 1440 10.1016/j.biocel.2008.12.009 1:CAS:528: DC%2BD1MXit1Krt78%3D
13. GM Lamm P Steinlein M Cotton G Christofori 1997 A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells Nucleic Acids Res 25 4855 4857 10.1093/nar/25.23.4855 1:CAS:528:DyaK1cXhslGkug%3D%3D (Pubitemid 27517224)
14. I Nicoletti G Migliorati MC Pagliacci F Grignani C Riccardi 1991 A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry J Immunol Methods 139 271 280 10.1016/0022-1759(91)90198-O 1:CAS:528:DyaK3MXltlenu7Y%3D
15. Y Otsuki 2000 Various methods of apoptosis detection Acta Histochem Cytochem 33 235 241 10.1267/ahc.33.235 1:CAS:528:DC%2BD3cXosFShu7c%3D (Pubitemid 32010406)
16. S Pandey PR Walker M Sikorska 1994 Separate pools of endonuclease activity are responsible for inter-nucleosomal and high-molecular-mass DNA fragmentation during apoptosis Biochem cell Biol (biochimie et biologie cellulaire) 72 625 629 10.1139/o94-082 1:CAS:528:DyaK2MXmtFSgsrg%3D
17. F Rosl 1992 A simple and rapid method for detection of apoptosis in human cells Nucleic Acids Res 20 5243 10.1093/nar/20.19.5243 1:STN:280: DyaK3s%2Fislarsg%3D%3D
18. A Saraste K Pulkki 2000 Morphological and biochemical hallmarks of apoptosis Cardiovasc Res 45 528 537 10.1016/S0008-6363(99)00384-3 1:CAS:528:DC%2BD3cXltVGlsg%3D%3D (Pubitemid 30047119)
19. R Sgonc J Guber 1998 Apoptosis detection: an overview Exp Gerontol 33 525 533 10.1016/S0531-5565(98)00031-X 1:STN:280:DyaK1M%2Fgs1Wmuw%3D%3D (Pubitemid 128370097)
20. NP Singh 2000 A simple method for accurate estimation of apoptotic cells Exp Cell Res 256 328 337 10.1006/excr.2000.4810 1:CAS:528:DC%2BD3cXitVWqtbg%3D (Pubitemid 30211196)
21. S Suman D Khaitan U Pati RK Seth S Chandna 2009 Stress response of a p53 homologue in the radioresistant Sf9 insect cells Int J Radiat Biol 85 238 249 10.1080/09553000902748591 1:CAS:528:DC%2BD1MXktFSmtbk%3D
22. S Tanuma D Shiokawa Y Tanimoto M Ikekita H Sakagami M Takada S Fukuda M Kochi 1993 Benzylideneascorbate induces apoptosis in L029 tumor cells Biochem Biophys Res Commun 15 29 35 10.1006/bbrc.1993.1780 (Pubitemid 23237896)
23. WG Telford LE King PJ Fraker 1991 Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow-cytometry Cell Prolif 24 447 459 10.1111/j.1365-2184.1991.tb01173.x 1:CAS:528:DyaK38XpvFGhsw%3D%3D
24. WG Telford LE King PJ Fraker 1992 Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry Cytometry 13 137 143 10.1002/cyto.990130205 1:CAS:528: DyaK38XitFSmtr8%3D
25. SR Umansky BR Korol PA Nelipaovich 1981 In vivo DNA degradation in the thymocytes of gamma-irradiated or hydrocortisone-treated rats Biochim Biophys Acta 655 281 290
26. G Wang Y Gong FJ Burczynski BB Hasinoff 2008 Cell lysis with dimethyl sulphoxide produces stable homogeneous solutions in the dichlorofluorescein oxidative stress assay Free Radic Res 42 435 441 10.1080/10715760802074462 1:CAS:528:DC%2BD1cXnsVKnsrY%3D (Pubitemid 351935157)
27. MC Willingham 1999 Cytochemical methods for the detection of apoptosis J Histochem Cytochem 47 1101 1109 10.1177/002215549904700901 1:CAS:528: DyaK1MXmtVals70%3D (Pubitemid 29418314)
28. Z Zhuang F Fei Y Chen W Jin 2008 Suberoyl bis-hydroxamic acid induces p53-dependent apoptosis of MCF-7 breast cancer cells Acta Pharmacol Sin 29 1459 1466 10.1111/j.1745-7254.2008.00906.x 1:CAS:528:DC%2BD1MXisFyqtQ%3D%3D