Fluorescence resonance energy transfer assay for high-throughput screening of ADAMTS1 inhibitors

Molecules

Volumn 16, Issue 12, 2011, Pages 10709-10721

  Peng, Jianhao ^a   Gong, Lili ^a   Si, Kun ^b   Bai, Xiaoyu ^a   Du, Guanhua ^a  

^a INSTITUTE OF MATERIA MEDICA   (China)

^b Case Western Reserve University   (United States)

Author keywords

ADAMTS1; Drug discovery; Fluorescence resonance energy transfer; High throughput screening; Inhibitor

Indexed keywords

ADAM PROTEIN; HYBRID PROTEIN; PEPTIDE; PROTEINASE INHIBITOR;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; HUMAN; IC 50; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PH; PROTEIN DEGRADATION; TEMPERATURE;

ADAM PROTEINS; AMINO ACID SEQUENCE; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROGEN-ION CONCENTRATION; INHIBITORY CONCENTRATION 50; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEASE INHIBITORS; PROTEOLYSIS; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

EID: 84555178946     PISSN: None     EISSN: 14203049     Source Type: Journal    
DOI: 10.3390/molecules161210709     Document Type: Article

References (25)

1. Tang, B.L. ADAMTS: A novel family of extracellular matrix proteases. Int. J. Biochem. Cell Biol. 2001, 33, 33-44. (Pubitemid 32146465)
2. Mitani, H.; Takahashi, I.; Onodera, K.; Bae, J.W.; Sato, T.; Takahashi, N.; Sasano, Y.; Igarashi, K.; Mitani, H. Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats. Histochem. Cell Biol. 2006, 126, 371-380.
3. Pockert, A.J.; Richardson, S.M.; Le Maitre, C.L.; Lyon, M.; Deakin, J.A.; Buttle, D.J.; Freemont, A.J.; Hoyland, J.A. Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration. Arthritis Rheum. 2009, 60, 482-491.
4. Song, R.H.; Tortorella, M.D.; Malfait, A.M.; Alston, J.T.; Yang, Z.; Arner, E.C.; Griggs, D.W. Aggrecan degradation in human articular cartilage explants is mediated by both ADAMTS-4 and ADAMTS-5. Arthritis Rheum. 2007, 56, 575-585.
5. Kuno, K.; Terashima, Y.; Matsushima, K. ADAMTS-1 is an active metalloproteinase associated with the extracellular matrix. J. Biol. Chem. 1999, 274, 18821-18826.
6. Kuno, K.; Kanada, N.; Nakashima, E.; Fujiki, F.; Ichimura, F.; Matsushima, K. Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene. J. Biol. Chem. 1997, 272, 556-562. (Pubitemid 27021194)
7. Kuno, K.; Matsushima, K. ADAMTS-1 protein anchors at the extracellular matrix through the thrombospondin type I motifs and its spacing region. J. Biol. Chem. 1998, 273, 13912-13917. (Pubitemid 28268445)
8. Rodriguez-Manzaneque, J.C.; Milchanowski, A.B.; Dufour, E.K.; Leduc, R.; Iruela-Arispe, M.L. Characterization of METH-1/ADAMTS1 processing reveals two distinct active forms. J. Biol. Chem. 2000, 275, 33471-33479.
9. Liu, Y.J.; Xu, Y.; Yu, Q. Full-length ADAMTS-1 and the ADAMTS-1 fragments display pro- and antimetastatic activity, respectively. Oncogene 2006, 25, 2452-2467.
10. Kuno, K.; Okada, Y.; Kawashima, H.; Nakamura, H.; Miyasaka, M.; Ohno, H.; Matsushima, K. ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan. FEBS Lett. 2000, 478, 241-245. (Pubitemid 30621625)
11. Rodriguez-Manzaneque, J.C.; Westling, J.; Thai, S.N.; Luque, A.; Knauper, V.; Murphy, G.; Sandy, J.D.; Iruela-Arispe, M.L. ADAMTS1 cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors. Biochem. Biophys. Res. Commun. 2002, 293, 501-508. (Pubitemid 34694236)
12. Gooljarsingh, L.T.; Lakdawala, A.; Coppo, F.; Luo, L.; Fields, G.B.; Tummino, P.J.; Gontarek, R.R. Characterization of an exosite binding inhibitor of matrix metalloproteinase 13. Protein Sci. 2008, 17, 66-71.
13. Nagase, H.; Kashiwagi, M. Aggrecanases and cartilage matrix degradation. Arthritis Res. Ther. 2003, 5, 94-103. (Pubitemid 36849755)
14. Lee, N.V.; Rodriguez-Manzaneque, J.C.; Thai, S.N.; Twal, W.O.; Luque, A.; Lyons, K.M.; Argraves, W.S.; Iruela-Arispe, M.L. Fibulin-1 acts as a cofactor for the matrix metalloprotease ADAMTS-1. J. Biol. Chem. 2005, 280, 34796-34804. (Pubitemid 41504613)
15. Tortorella, M.D.; Malfait, F.; Barve, R.A.; Shieh, H.S.; Malfait, A.M. A review of the ADAMTS family, pharmaceutical targets of the future. Curr. Pharm. Des. 2009, 15, 2359-2374.
16. Vankemmelbeke, M.N.; Jones, G.C.; Fowles, C.; Ilic, M.Z.; Handley, C.J.; Day, A.J.; Knight, C.G.; Mort, J.S.; Buttle, D.J. Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters. Eur. J. Biochem. 2003, 270, 2394-2403. (Pubitemid 36645357)
17. Pasternak, B.; Aspenberg, P. Metalloproteinases and their inhibitors-diagnostic and therapeutic opportunities in orthopedics. Acta Orthop. 2009, 80, 693-703.
18. Li, X.Y.; Takasaki, C.; Satoh, Y.; Kimura, S.; Yasumoto, K.; Sogawa, K. Expression, purification and characterization of human PHD1 in Escherichia coli. J. Biochem. 2008, 144, 555-561.
19. Lind, T.; Birch, M.A.; McKie, N. Purification of an insect derived recombinant human ADAMTS-1 reveals novel gelatin (type I collagen) degrading activities. Mol. Cell. Biochem. 2006, 281, 95-102. (Pubitemid 43040128)
20. Takasugi, M.; Ishikawa, S.; Nagao, S.; Masamune, T. Studies on phytoalexins of the moraceae. Part 12. Albafuran-C, a natural diels-alder adduct of a dehydroprenyl-2-phenylbenzofuran with a chalcone from mulberry. Chem. Lett. 1982, 8, 1223-1224.
21. Hano, Y.; Takizawa, S.; Mizuno, E.; Nomura, T. Structure of kuwanon-P, a new diels-alder type adduct from the Root bark of the cultivated mulberry tree (morus-lhou (ser) koidz). Chem. Pharm. Bull. 1983, 31, 2936-2939.
22. Hirakura, K.; Hano, Y.; Fukai, T.; Nomura, T.; Uzawa, J.; Fukushima, K. Constituents of the cultivated mulberry tree. 21. Structures of 3 new natural diels-alder type adducts, kuwanon-P and kuwanon-X, and mulberrofuran-J, from the cultivated mulberry tree (morus-lhou koidz). Chem. Pharm. Bull. 1985, 33, 1088-1096.
23. Dai, S.J.; Mi, Z.M.; Ma, Z.B.; Li, S.; Chen, R.Y.; Yu, D.Q. Bioactive diels-alder type adducts from the stem bark of morus macroura. Planta Med. 2004, 70, 758-763. (Pubitemid 39178938)
24. Zhang, J.H.; Chung, T.D.; Oldenburg, K.R. A Simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 1999, 4, 67-73. (Pubitemid 29278954)
25. Zhang, R.Y.; Qin, Y.; Lv, X.Q.; Wang, P.; Xu, T.Y.; Zhang, L.; Miao, C.Y. A fluorometric assay for high-throughput screening targeting nicotinamide phosphoribosyltransferase. Anal. Biochem. 2011, 412, 18-25.