Ratiometric fluorescence imaging of dual bio-molecular events in single living cells using a new FRET pair mVenus/mKOκ-based biosensor and a single fluorescent protein biosensor

Biosensors and Bioelectronics

Volumn 31, Issue 1, 2012, Pages 292-298

  Su, Ting ^a,b   Zhang, Zhihong ^a,b   Luo, Qingming ^a,b  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Biosensor; Fluorescent protein; Grx1 roGFP2; MKO ; MVenus; Ratiometric fluorescent imaging

Indexed keywords

BIO-MOLECULAR; EPIDERMAL GROWTH FACTORS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT PROTEIN; GLUTATHIONES; GRX1-ROGFP2; IMAGING CONDITIONS; LIVING CELL; LOW DEGREE; MOLECULAR EVENTS; MULTIPARAMETERS; MVENUS; NON-INVASIVE; RATIOMETRIC; RATIOMETRIC FLUORESCENCE; RATIOMETRIC IMAGING; REDOX POTENTIALS; REDOX SYSTEMS; SIMULTANEOUS IMAGING; SINGLE CELLS;

CELLS; ENERGY TRANSFER; FLUORESCENCE; PEPTIDES; REDOX REACTIONS;

BIOSENSORS;

CALCIUM ION; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYE; GLUTAREDOXIN; GLUTAREDOXIN 1; GLUTATHIONE; GREEN FLUORESCENT PROTEIN; HYDROGEN PEROXIDE; ORANGE FLUORESCENT PROTEIN; PROTEIN TYROSINE KINASE; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

ARTICLE; BIOSENSOR; CALCIUM SIGNALING; CONTROLLED STUDY; FLUORESCENCE IMAGING; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGE ANALYSIS; IMAGE PROCESSING; MOLECULAR IMAGING; OXIDATION REDUCTION POTENTIAL; RATIOMETRIC FLUORESCENCE IMAGING; SIGNAL PROCESSING; SIGNAL TRANSDUCTION;

BIOSENSING TECHNIQUES; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; PROTEIN ARRAY ANALYSIS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84455205610     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2011.10.034     Document Type: Article

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