Chromium-induced tropane alkaloid production and H6H gene expression in Atropa belladonna L. (Solanaceae) invitro-propagated plantlets

Plant Physiology and Biochemistry

Volumn 52, Issue , 2012, Pages 98-103

  Vakili, Bahareh ^a   Karimi, Farah ^a   Sharifi, Mozafar ^b   Behmanesh, Mehrdad ^b  

^a SHAHED UNIVERSITY   (Iran)

^b TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

Atropa belladonna L.; H6h; Hyoscyamine; Scopolamine; Tropane alkaloids

Indexed keywords

ALKALOID; ATROPINE; CHLOROPHYLL; CHOLINERGIC RECEPTOR BLOCKING AGENT; CHROMIUM; HYOSCYAMINE (6S) DIOXYGENASE; HYOSCYAMINE (6S)-DIOXYGENASE; MIXED FUNCTION OXIDASE; PROLINE; SCOPOLAMINE BROMIDE; TROPANE DERIVATIVE;

ARTICLE; ATROPA BELLADONNA; DOSE RESPONSE; DRUG EFFECT; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM;

ALKALOIDS; ATROPA BELLADONNA; ATROPINE; CHLOROPHYLL; CHOLINERGIC ANTAGONISTS; CHROMIUM; DOSE-RESPONSE RELATIONSHIP, DRUG; MIXED FUNCTION OXYGENASES; PROLINE; SCOPOLAMINE HYDROBROMIDE; TROPANES;

ATROPA BELLADONNA; SOLANACEAE;

EID: 84255188923     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2011.12.003     Document Type: Article

References (29)

1. Kang S.M., Jung H., Kang Y., Yun D., Bahk J., Yang J., Choi M. Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci. 2004, 166:745-751.
2. Palazón J., Navaro-Ocaña A., Hernandez-Vazquez L., Mirjalili M.H. Aplication of metabolic engineering to the production of scopolamine. Molecules 2008, 13:1722-1742.
3. Hashimoto T., Nakajima K., Ongena G., Yamada Y. Two tropinone reductases with distinct stereospecifities from cultured roots of Hyoscyamus niger. Plant Physiol. 1992, 100:836-845.
4. Matsuda J., Okabe S., Hashimoto T., Yamada Y. Molecular cloning of hyoscyamine GB-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger. Biol. Chem. 1991, 266:9460-9464.
5. Hashimoto T., Matsuda J., Yamada Y. Two-step epoxidatiun of hyascyamine to scopolamine is catalyzed by bifunctional hyoscyamine 6β -hydroxylase. FIBS 1993, 329:35-39.
6. El Jaber-Vazdekis N., Gonzalez C., Ravelo A.G., Zad́rate R. Cloning, characterization and analysis of expression profiles of a cDNA encoding a hyoscyamine 6 β -hydroxylase (H6H) from Atropa baetica Willk. Plant Physiol. Biochem. 2009, 47(1):20-25.
7. Palazón J., Moyano E., Cusidó R.M., Bonfill M., Oksman-Caldentey K.M., Piñol M.T. Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Sci. 2003, 165:1289-1295.
8. Zad́rate R., El Jaber-Vazdekis N., Medina B., Ravelo A.G. Tailoring tropane alkaloid accumulation in transgenic hairy roots of Atropa baetica by over-expressing the gene encoding hyoscyamine 6b-hydroxylase. Biotechnol. Lett. 2006, 28:1271-1277.
9. Pitta-Alvarez S.I., Spollansky T.C., Giulietti A.M. The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzym. Microb. Technol. 2000, 26:252-258.
10. Vernay P., Gauthier-Moussard C., Jean L., Bordas F., Faure O., Ledoigt G., Hitmi A. Effect of chromium species on phytochemical and physiological parameters in Datura innoxia. Chemosphere 2008, 72:763-771.
11. Murashige T., Skoog F. Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 1962, 15:473-497.
12. Mano Y., Nabeshima S., Matsui C., Ohkawa H. Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Agric. Biol. Chem. 1986, 50:2715-2722.
13. Arnon D.I. Copper enzymes in isolated chloroplast Polyphennoloxidase in Beta vulgaris. Plant Physiol. 1949, 24:1-15.
14. Bates S., Waldren R.P., Teare I.D. Rapid determination of the free proline in water stress studies. Plant Soil 1973, 39:205-208.
15. Panda S.K., Choudhury S. Chromium stress in plants. Braz. J. Plant Physiol. 2005, 17:95-102.
16. Van Assche F.V., Clijsters H. Effects of metals on enzyme activity in plants. Plant Cell Environ. 1990, 13:95-206.
17. Vajpayee R.D., Rai U.N., Ali M.B., Tripati R.D., Singh S.N. Chromium induced physiologic changes in Vallisneria spiralis L. and its role in phytoremediation of tannery effluents. Bull. Environ. Contam. Toxicol. 2001, 67:246-256.
18. Tripathi B.N., Gaur J.P. Relationship between Copper and Zinc-induced oxidative stress and proline accumulation in Scenedesmus sp. Planta 2004, 219:397-404.
19. Rai V., Vajpayee P., Singh S.N., Mehrotra S. Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L. Plant Sci. 2004, 167:1159-1169.
20. Ashraf M., Foolad M.R. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot. 2007, 59:206-216.
21. Molinari H.B.C., Marur C.J., Darcos E., Bespalhok M.K., Carvalho P.J., Filho J.C., Pereira L.F.P., Vieira L.G.E. Evaluation of the stress-inducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol. Plant 2007, 130:218-229.
22. Zhang L., Ding R., Chai Y., Bonfill M., Moyano E., Oksman-Caldentey K.M., Xu T., Pi Y., Wang Z., Zhang H., Kai G., Liao Z., Sun X., Tang K. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. PNAS 2004, 17:6786-6791.
23. Yun D.J., Hashimoto T., Yamada Y. Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition. Proc. Natl. Acad. Sci. 1992, 89:11799-11803.
24. Baricevic D., Umek A., Kreft S., Maticic B., Zupancic A. Effect of water stress and nitrogen fertilization on the content of hyoscyamine and scopolamine in the roots of deadly nightshade (Atropa belladonna). Environ. Exp. Bot. 1999, 42:17-24.
25. Flores H.E., Vivanco J.M., Loyola-Vargas V.M. Radicle' biochemistry: the biology of root-specific metabolism. Trends Plant Sci. 1999, 4:220-226.
26. Rothe G., Hachiya A., Yamada Y., Hashimoto T., Draeger B. Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase. J.Exp. Bot. 2003, 54:2065-2070.
27. Kanegae T., Kajiya H., Amano Y., Hashimoto T., Yamada Y. Species-dependent expression of Hyoscyamine 6 β -Hydroxylase gene in the the pericycle. Plant Physiol. 1994, 105:483-490.
28. Suzuki K., Yun D.J., Chen X.Y., Yamada Y., Hashimoto T. An Atropa belladonna hyoscyamine 6 β-hydroxylase gene is differentially expressed in the root pericycle and anthers. Plant Mol. Biol. 1999, 40:141-152.
29. Kai G., Chen J., Li L., Zhou G., Zhou L., Zhang L., Chen Y., Zhao L. Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6β-hydroxylase from roots of Anisodus acutangulus. Biochem. Mol. Biol. 2007, 40:715-722.