Cloning, characterization and analysis of expression profiles of a cDNA encoding a hyoscyamine 6β-hydroxylase (H6H) from Atropa baetica Willk

Plant Physiology and Biochemistry

Volumn 47, Issue 1, 2009, Pages 20-25

  el Jaber Vazdekis, Nabil ^a,b   González, Celedonio ^a   Ravelo, Ángel G ^a,b   Zárate, Rafael ^a,b  

^a UNIVERSIDAD DE LA LAGUNA   (Spain)

^b HOSPITAL UNIVERSITARIO NUESTRA SEÑORA DE CANDELARIA   (Spain)

Author keywords

Atropa baetica; Copy number; Expression profile; Hyoscyamine 6 hydroxylase

Indexed keywords

COMPLEMENTARY DNA; HYOSCYAMINE (6S) DIOXYGENASE; HYOSCYAMINE (6S)-DIOXYGENASE; MIXED FUNCTION OXIDASE; SCOPOLAMINE; VEGETABLE PROTEIN;

ARTICLE; ATROPA; BIOSYNTHESIS; CLASSIFICATION; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLANT ROOT;

ATROPA; BASE SEQUENCE; DNA, COMPLEMENTARY; GENE EXPRESSION PROFILING; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANT PROTEINS; PLANT ROOTS; SCOPOLAMINE; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC;

ANISODUS TANGUTICUS; ATROPA; ATROPA BAETICA; ATROPA BELLADONNA; HYOSCYAMUS; HYOSCYAMUS NIGER;

EID: 57649103111     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2008.09.004     Document Type: Article

References (26)

1. Dhar A.K., and Bhat B.K. Ontogenic variability in alkaloid synthesis and other morphological characters in five genotypes of belladonna. J. Nat. Prod 45 (1982) 525-531
2. Doerkschmitz K., Witte K., and Alfermann A.W. Tropane alkaloid patterns in plants and hairy roots of Hyoscyamus albus. Phytochemistry 35 (1994) 107-111
3. Emanuelsson O., Nielsen H., Brunak S., and Von Heijne G. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J. Mol. Biol. 300 (2000) 1005-1016
4. Evans W.C. Tropane alkaloids from Solanaceae. In: Hawkes J.G., Lester R.N., and Skelding A.D. (Eds). The Biology and Taxonomy of the Solanaceae. Linnean Society Symposium Series No 7 (1979), Academic Press, London 241-254
5. Fliniaux M.A., Manceau F., and Jacquin-Dubreuid A. Simultaneous analysis of l-hyoscyamine, l-scopolamine and dl-tropic acid in plant material by reversed-phase high-performance liquid chromatography. J. Chromatogr 644 (1993) 193-197
6. Geourjon C., and Deléage G. SOPMA: Significant improvement in protein secondary structure prediction by consensus prediction from multiple alignments. Cabios 11 (1995) 681-684
7. Hashimoto T., Hayashi A., Amano Y., Kohno J., Iwanari H., Usada S., and Yamada Y. Hyoscyamine 6β-Hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is located at the pericycle of the root. J. Biol. Chem. 266 7 (1991) 4648-4653
8. Hashimoto T., Kanegae T., Kajiya H., Matsuda J., and Yamada Y. Expression of hyoscyamine 6β-hydroxylase in plants and E. Coli. Curr. Plant Sci. Biotechnol. Agric 22 (1995) 605-609
9. Kai G., Chen J., Li L., Zhou G., Zhou L., Zhang L., Chen Y., and Zhao L. Molecular cloning and characterization of new cDNA encoding hyoscyamine 6β-hydroxylase from roots of Anisodus acutangulus. J. Biochem. Mol. Biol. 40 (2007) 715-722
10. Kanegae T., Kajiya H., Amano Y., Hashimoto T., and Yamada Y. Species-Dependent expression of hyoscyamine 6β-hydroxylase gene in the pericycle. Plant Physiol 105 (1994) 483-490
11. Liu T., Zhu P., Cheng K.D., Meng C., and He H.X. Molecular cloning, expression and characterization of hyoscyamine 6β-hydroxylase from hairy roots of Anisodus tanguticus. Planta Med 71 (2005) 249-253
12. Matsuda J., Okabe S., Hashimoto T., and Yamada Y. Molecular cloning of Hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger. J. Biol. Chem. 266 15 (1991) 9460-9464
13. Moyano E., Palazón J., Bonfill M., Osuna L., Cusido R.M., and Oksman-Caldentey K.M. Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures. Plant Physiol 164 (2007) 521-524
14. Oksman-Caldentey K.M. Tropane and nicotine alkaloid biosynthesis-novel approaches towards biotechnological production of plant-derived pharmaceuticals. Curr. Pharm. Biotechnol 8 (2007) 203-210
15. Palazón J., Moyano E., Cusidó R.M., Bonfill M., Oksman-Caldentey K.M., and Piñol M.T. Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Sci. 165 (2003) 1289-1295
16. Prescott A.G., and John P. Dioxygenases: molecular structure and role in plant metabolism, Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996) 245-271
17. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual. 3rd Edition (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor
18. Suzuki K., Yun D.J., Chen X.Y., Yamada Y., and Hashimoto T. An Atropa belladonna hyoscyamine 6β-hydroxylase gene is differentially expressed in the root pericycle and anthers. Plant Mol. Biol. 40 (1998) 141-152
19. Tamura K., Dudley J., Nei M., and Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24 (2007) 1596-1599
20. Tyker V.E., Brady L.R., and Robbers J.E. Pharmacognosy. 9th Edition (1988), Lea and Febiger, Philadelphia, USA
21. Willaman J.J., and Li H.L. Alkaloid bearing plants and their contained alkaloids. J. Nat. Prod 30 (1970) 1-4
22. Yun D.J., Hashimoto T., and Yamada Y. Metabolic engineering of medicinal plants: Transgenic Atropa belladonna with an improved alkaloid composition. Proc. Natl. Acad. Sci. USA 89 (1992) 11799-11803
23. Zárate R. Tropane alkaloid production by Agrobacterium rhizogenes transformed hairy root cultures of Atropa baetica Willk (Solanaceae). Plant Cell Rep 18 (1999) 418-423
24. Zárate R., El Jaber-Vazdekis N., Medina B., and Ravelo A.G. Tailoring tropane alkaloid accumulation in transgenic hairy roots of Atropa baetica by over-expressing the gene encoding hyoscyamine 6β-hydroxylase. Biotechnol. Lett. 28 (2006) 1271-1277
25. Zárate R., Hermosin B., Cantos M., and Troncoso A. Tropane alkaloid distribution in Atropa baetica plants. J. Chem. Ecol 23 (1997) 2059-2066
26. Zárate R., Cantos M., and Troncoso A. Induction and development of adventitious shoots of Atropa baetica as a mean of propagation. Euphytica 94 (1997) 361-366