Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase

Journal of Experimental Botany

Volumn 54, Issue 390, 2003, Pages 2065-2070

  Rothe, Grit ^a   Hachiya, Akira ^b   Yamada, Yasuyuki ^b   Hashimoto, Takashi ^b   Dräger, Birgit ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Atropa belladonna; Calystegine; Hyoscyamine; Overexpression; Putrescine N methyltransferase; Scopolamine; Tropane alkaloids

Indexed keywords

ENZYMES; GENES; SUGAR (SUCROSE);

ROOT CULTURE;

PLANT CELL CULTURE;

ATROPA BELLADONNA; ATROPINE; BELLADONNA ALKALOIDS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GENOME, PLANT; INDOLEACETIC ACIDS; METHYLTRANSFERASES; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT FUSION PROTEINS; SCOPOLAMINE; TOBACCO; TROPANES;

ATROPA; ATROPA BELLADONNA; EMBRYOPHYTA; EUKARYOTA; INSERTION SEQUENCES; NICOTIANA; NICOTIANA TABACUM;

EID: 0041374254     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/erg227     Document Type: Article

References (22)

1. Asano N, Yokoyama K, Sakurai M, et al. 2001. Dihydroxynortropane alkaloids from calystegine-producing plants. Phytochemistry 57, 721-726.
2. Baldwin IT. 1998. Jasmonate-induced responses are costly but benefit plants under attack in native populations. Proceedings of the National Academy of Sciences, USA 95, 8113-8118.
3. Baldwin IT, Zhang ZP, Diab N, Ohnmeiss TE, McCloud ES, Lynds GY, Schmelz EA. 1997. Quantification, correlations and manipulation of wound-induced changes in jasmonic acid and nicotine in Nicotiana sylvestris. Planta 201, 397-404.
4. Biondi S, Fornale S, Oksman-Caldentey KM, Eeva M, Agostani S, Bagni N. 2000. Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures. Plant Cell Reports 19, 691-697.
5. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.
6. Draeger B, Van-Almssick A, Mrachatz G. 1995. Distribution of calystegines in several Solanaceae. Planta Medica 61, 577-579.
7. Feth F, Wagner R, Wagner KG. 1986. Regulation in tobacco callus of enzyme activities of the nicotine pathway. I. The route ornithine to methylpyrroline. Planta 168, 402-407.
8. Hashimoto T, Mitani A, Yamada Y. 1990. Diamine oxidase from cultured roots of Hyoscyamus niger: its function in tropane alkaloid biosynthesis. Plant Physiology 93, 216-221.
9. Hashimoto T, Tamaki K, Suzuki K, Yamada Y. 1998. Molecular cloning of plant spermidine synthases. Plant and Cell Physiology 39, 73-79.
10. Hashimoto T, Yukimune Y, Yamada Y. 1986. Tropane alkaloid production in Hyoscyamus root cultures. Journal of Plant Physiology 124, 61-76.
11. Hibi N, Higashiguchi S, Hashimoto T, Yamada Y. 1994. Gene expression in tobacco low-nicotine mutants. The Plant Cell 6, 723-735.
12. Imanishi S, Hashizume K, Nakakita M, Kojima H, Matsubayashi Y, Hashimoto T, Sakagami Y, Yamada Y, Nakamura K. 1998. Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures. Plant Molecular Biology 38, 1101-1111.
13. Moyano E, Fornale S, Palazon J, Cusido RM, Bagni N, Pinol MT. 2002. Alkaloid production in Duboisia hybrid hairy root cultures overexpressing the pmt gene. Phytochemistry 59, 697-702.
14. Moyano E, Jouhikainen K, Tammela P, Palazon J, Cusido RM, Pinol MT, Teeri HT, Oksman-Caldentey KM. 2003. Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. Journal of Experimental Botany 54, 203-211.
15. Nash RJ, Rothschild M, Porter EA, Watson AA, Waigh RD, Waterman PG. 1993. Calystegines in Solanum and Datura species and the death's-head hawk-moth (Acherontia atropus). Phytochemistry 34, 1281-1283.
16. Rothe G, Dräger B. 2002. Tropane alkaloids - metabolic response to carbohydrate signal in root cultures of Atropa belladonna. Plant Science 163, 979-985.
17. Rothe G, Garske U, Dräger B. 2001. Calystegines in root cultures of Atropa belladonna respond to sucrose, not to elicitation. Plant Science 160, 1043-1053.
18. Sato F, Hashimoto T, Hachiya A, Tamura K, Choi K-B, Morishige T, Fujimoto H, Yamada Y. 2001. Metabolic engineering of plant alkaloid biosynthesis. Proceedings of the National Academy of Sciences, USA 98, 367-372.
19. Sevon N, Draeger B, Hiltunen R, Oksman-Caldentey KM. 1997. Characterization of transgenic plants derived from hairy roots of Hyoscyamus muticus. Plant Cell Reports 16, 605-611.
20. Shoji T, Yamada Y, Hashimoto T. 2000. Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris. Plant and Cell Physiology 41, 831-839.
21. Suzuki K, Yamada Y, Hashimoto T. 1999. Expression of Atropa belladonna putrescine N-methyltransferase gene in root pericycle. Plant and Cell Physiology 40, 289-297.
22. Tiburcio AF, Kaur SR, Galston AW. 1986. Polyamine metabolism ans osmotic stress. II. Improvement of oat protoplasts by an inhibitor of arginine decarboxylase. Plant Physiology 82, 375-378.