Biochip-based detection of KRAS mutation in non-small cell lung cancer

International Journal of Molecular Sciences

Volumn 12, Issue 12, 2011, Pages 8530-8538

  Kriegshäuser, Gernot ^a   Fabjani, Gerhild ^b,c   Ziegler, Barbara ^b   Zöchbauer Müller, Sabine ^d   End, Adelheid ^d   Zeillinger, Robert ^b,c  

^a VIENNALAB DIAGNOSTICS GMBH   (Austria)

^b UNIV KLIN FÜR FRAUENHEILKUNDE   (Austria)

^c Cluster Translational Oncology   (Austria)

^d MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Biochip hybridization; KRAS; Mutation detection; Non small cell lung cancer

Indexed keywords

ADULT; AGED; ARTICLE; BIOCHIP; CODON; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA SEQUENCE; FEMALE; GENE MUTATION; HUMAN; HUMAN TISSUE; LIMIT OF DETECTION; LUNG NON SMALL CELL CANCER; MALE; MUTATIONAL ANALYSIS; ONCOGENE K RAS; POLYMERASE CHAIN REACTION; CARCINOMA, NON-SMALL-CELL LUNG; CASE CONTROL STUDY; DNA MICROARRAY; EVALUATION STUDY; GENETICS; LUNG NEOPLASMS; MIDDLE AGED; MUTATION; VERY ELDERLY;

KRAS PROTEIN, HUMAN; ONCOPROTEIN; RAS PROTEIN; TUMOR MARKER;

ADULT; AGED; AGED, 80 AND OVER; CARCINOMA, NON-SMALL-CELL LUNG; CASE-CONTROL STUDIES; FEMALE; HUMANS; LUNG NEOPLASMS; MALE; MIDDLE AGED; MUTATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROTO-ONCOGENE PROTEINS; RAS PROTEINS; TUMOR MARKERS, BIOLOGICAL;

EID: 84055217620     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12128530     Document Type: Article

References (21)

1. Blons, H.; Pallier, K.; Le Corre, D.; Danel, C.; Tremblay-Gravel, M.; Houdayer, C.; Fabre-Guillevin, E.; Riquet, M.; Dessen, P.; Laurent-Puig, P. Genome wide SNP comparative analysis between EGFR and KRAS mutated NSCLC and characterization of two models of oncogenic cooperation in non-small cell lung carcinoma. BMC Med. Genomics 2008, 1, 25.
2. Rusch, V.; Baselga, J.; Cordon-Cardo, C.; Orazem, J.; Zaman, M.; Hoda, S.; McIntosh, J.; Kurie, J.; Dmitrovsky, E. Differential expression of the epidermal growth factor receptor and its ligands in primary non-small cell lung cancers and adjacent benign lung. Cancer Res. 1993, 53, 2379-2385.
3. Adjei, A.A. Blocking oncogenic Ras signaling for cancer therapy. J. Natl. Cancer Inst. 2001, 93, 1062-1074.
4. Langer, C.J. Roles of EGFR and KRAS Mutations in the Treatment of Pateintes with Non-Small-Cell Lung Cancer. Pharm. Ther. 2011, 36, 263-279.
5. Keohavong, P.; Mady, H.H.; Gao, W.M.; Siegfried, J.M.; Luketich, J.D.; Melhem, M.F. Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung cancer patients. Br. J. Cancer 2001, 85, 235-241.
6. Herbst, R.S.; Sandler, A. Bevacizumab and erlotinib: a promising new approach to the treatment of advanced NSCLC. Oncologist 2008, 13, 1166-1176.
7. Pao, W.; Miller, V.; Zakowski, M.; Doherty, J.; Politi, K.; Sarkaria, I.; Singh, B.; Heelan, R.; Rusch, V.; Fulton, L.; et al. EGF receptor gene mutations are common in lung cancers from "never smokers" and are associated with the sensitivity of tumors to gefitinib and erlotinib. Proc. Natl. Acad. Sci. USA 2004, 101, 13306-13311.
8. Pao, W.; Wang, T.Y.; Riely, G.J.; Miller, V.A.; Pan, Q.; Ladanyi, M.; Zakowski, M.F.; Hellan, R.T.; Kris, M.-G.; Varmus, H.E. KRAS Mutations and Primary Resistance of Lung Adenocarcinomas to Gefitinib or Erlotinib. PLoS Med. 2005, 2, e17.
9. Fabjani, G.; Kriegshaeuser, G.; Schuetz, A.; Prix, L.; Zeillinger, R. Biochip for K-ras mutation screening in ovarian cancer. Clin. Chem. 2005, 51, 784-787.
10. Auner, V.; Kriegshäuser, G.; Tong, D.; Horvat, R.; Reinthaller, A.; Mustea, A.; Zeillinger, R. KRAS mutation analysis in ovarian samples using a high sensitivity biochip assay. BMC Cancer 2009, 9, e111.
11. Kriegshäuser, G.; Auner, V.; Schuster, E.; Holzer, B.; Oberkanins, C.; Horvat, R.; Speiser, P.; Zeillinger, R. KRAS mutation analysis in genomic DNA isolated from formalin-fixed paraffin-embedded ovarian tissue: evaluation of a strip-based reverse-hybridisation assay. J. Clin. Pathol. 2011, 64, 252-256.
12. Keohavong, P.; DeMichele, M.A.; Melacrinos, A.C.; Landreneau, R.J.; Weyant, R.J.; Siegfried, J.M. Detection of K-ras mutations in lung carcinomas: Relationship to prognosis. Clin. Cancer Res. 1996, 2, 411-418.
13. Do, H.; Krypuy, M.; Mitchell, P.L.; Fox, S.B.; Dobrovic, A. High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies. BMC Cancer 2008, 8, e142.
14. van Eijk, R.; Licht, J.; Schrumpf, M.; Yazdi, M.T.; Ruano, D.; Forte, G.I.; Nederlof, P.M.; Veselic, M.; Rabe, K.F.; Annema, J.T.; et al. Rapid KRAS, EGFR, BRAF and PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer Using Allele-Speific qPCR. PLoS One 2011, 6, e17791.
15. Kotoula, V.; Charalambous, E.; Biesmans, B.; Malousi, A.; Vrettou, E.; Fountzilas, G.; Karkavelas, G. Targeted KRAS mutation assessment on patient tumor histologic material in real time diagnostics. PLoS One 2009, 4, e7746.
16. Sarasqueta, A.F.; Moerland, E.; de Bruyne, H.; de raaf, H.; Vrancken, T.; van Lijnschoten, G.; van den Brule, A.J.C. SnaPshot and StripAssay as Valuable Alternatives to Direct Sequencing for KRAS Mutation Detection in Colon Cancer Routine Diagnostics. J. Mol. Diagn. 2011, 13, 199-205.
17. Krypuy, M.; Newnham, G.M.; Thomas, D.M.; Conron, M.; Dobrovic, A. High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS codon 12 and 13 mutations in non-small cell lung cancer. BMC Cancer 2006, 6, e295.
18. Ogino, S.; Kawasaki, T.; Brahmandam, M.; Yan, L.; Cantor, M.; Namgyal, C.; Mino-Kenudson, M.; Lauwers, G.Y.; Loda, M.; Fuchs, C.S. Sensitive sequencing method for KRAS mutation detection by Pyrosequencing. J. Mol. Diagn. 2005, 7, 413-421.
19. Tol, J.; Dijkstra, J.R.; Vink-Borger, M.E.; Nagtegaal, I.D.; Punt, C.J.; van Krieken, J.H.; Ligtenberg, M.J. High sensitivity of both sequencing and real-time PCR analysis of KRAS mutations in colorectal cancer tissue. J. Cell. Mol. Med. 2010, 14, 2122-2131.
20. Sambrook, J.; Fritsch, E.F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: New York, NY, USA, 1989.
21. Prix, L.; Uciechowski, P.; Böckmann, B.; Giesing, M.; Schuetz, A.J. Diagnostic biochip array for fast and sensitive detection of K-ras mutations in stool. Clin. Chem. 2002, 48, 428-435.