Interleukin-1α, -6, and -8 decrease Cdc42 activity resulting in loss of articular chondrocyte phenotype

Journal of Orthopaedic Research

Volumn 30, Issue 2, 2012, Pages 246-251

  Novakofski, Kira D ^a   Torre, Christopher J ^a   Fortier, Lisa A ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

actin; Cdc42; chondrocyte; IL 1; interleukin

Indexed keywords

AGGRECAN; COLLAGEN TYPE 2; COLLAGEN TYPE IIB; COLLAGENASE 3; CYTOCHALASIN D; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; INTERLEUKIN 1ALPHA; INTERLEUKIN 6; INTERLEUKIN 8; PROTEIN CDC42; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; ARTICULAR CARTILAGE; CELL GROWTH; CELL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; HORSE; MONOLAYER CULTURE; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION; STRESS FIBER;

ANIMALS; CARTILAGE, ARTICULAR; CDC42 GTP-BINDING PROTEIN; CHONDROCYTES; GTPASE-ACTIVATING PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HORSES; INTERLEUKIN-1ALPHA; INTERLEUKIN-6; INTERLEUKIN-8; PHENOTYPE; STRESS FIBERS;

EID: 83855164138     PISSN: 07360266     EISSN: 1554527X     Source Type: Journal    
DOI: 10.1002/jor.21515     Document Type: Article

References (38)

1. Eid K, Thornhill TS, Glowacki J,. 2006. Chondrocyte gene expression in osteoarthritis: correlation with disease severity. J Orthop Res 24: 1062-1068. (Pubitemid 43804942)
2. Kouri JB, Argüello C, Luna J, et al. 1998. Use of microscopical techniques in the study of human chondrocytes from osteoarthritic cartilage: an overview. Microsc Res Tech 40: 22-36. (Pubitemid 28028681)
3. Yamamoto K, Shishido T, Masaoka T, et al. 2005. Morphological studies on the ageing and osteoarthritis of the articular cartilage in C57 black mice. J Orthop Surg 13: 8-18.
4. Capin-Gutierrez N, Talamas-Rohana P, Gonzalez-Robles A, et al. 2004. Cytoskeleton disruption in chondrocytes from a rat osteoarthrosic (OA)-induced model: its potential role in OA pathogenesis. Histol Histopathol 19: 1125-1132. (Pubitemid 39386770)
5. Zwicky R, Baici A,. 2000. Cytoskeletal architecture and cathepsin B trafficking in human articular chondrocytes. Histochem Cell Biol 114: 363-372.
6. Tyler J, Saklatvala J,. 1985. Pig interleukin-1 (catabolin) induces resorption of cartilage proteoglycan and prevents synthesis of proteoglycan and collagen. Rheumatology 24: 150.
7. Novakofski K, Boehm A, Fortier L,. 2009. The small GTPase rho mediates articular chondrocyte phenotype and morphology in response to interleukin-1α and insulin-like growth factor-I. J Orthop Res 27: 58-564.
8. Nobes CD, Hall A,. 1995. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81: 53-62.
9. Puls A, Eliopoulos AG, Nobes CD, et al. 1999. Activation of the small GTPase Cdc42 by the inflammatory cytokines TNF (alpha) and IL-1, and by the Epstein-Barr virus transforming protein LMP1. J Cell Sci 112: 2983.
10. Hickery MS, Bayliss MT, Dudhia J, et al. 2003. Age-related changes in the response of human articular cartilage to IL-1α and transforming growth factor-β (TGF-β). J Biol Chem 278: 53063.
11. Murata M, Bonassar LJ, Wright M, et al. 2003. A role for the interleukin-1 receptor in the pathway linking static mechanical compression to decreased proteoglycan synthesis in surface articular cartilage. Arch Biochem Biophys 413: 229-235. (Pubitemid 36506812)
12. Taskiran D, Stefanovicracic M, Georgescu H, et al. 1994. Nitric-oxide mediates suppression of cartilage proteoglycan synthesis by interleukin-1. Biochem Biophys Res Commun 200: 142-148.
13. Ratcliffe A, Tyler JA, Hardingham TE,. 1986. Articular cartilage cultured with interleukin 1. Increased release of link protein, hyaluronate-binding region and other proteoglycan fragments. Biochem J 238: 571.
14. Henrotin YE, De Groote DD, Labasse AH, et al. 1996. Effects of exogenous IL-1 [beta], TNF [alpha], IL-6, IL-8 and LIF on cytokine production by human articular chondrocytes. Osteoarthritis Cartilage 4: 163-173. (Pubitemid 26334016)
15. Legendre F, Dudhia J, Pujol JP, et al. 2003. JAK/STAT but not ERK1/ERK2 pathway mediates interleukin (IL)-6/soluble IL-6R down-regulation of type II collagen, aggrecan core, and link protein transcription in articular chondrocytes. J Biol Chem 278: 2903.
16. Lotz M, Terkeltaub R, Villiger PM,. 1992. Cartilage and joint inflammation. regulation of IL-8 expression by human articular chondrocytes. J Immunol 148: 466.
17. Fortier LA, Kornatowski MA, Mohammed HO, et al. 2005. Age-related changes in serum insulin-like growth factor-I, insulin-like growth factor-I binding protein-3 and articular cartilage structure in thoroughbred horses. Equine Vet J 37: 37-42. (Pubitemid 40091054)
18. Nixon AJ, Lust G, Vernier-Singer M,. 1992. Isolation, propagation, and cryopreservation of equine articular chondrocytes. Am J Vet Res 53: 2364-2370.
19. Clutterbuck AL, Mobasheri A, Shakibaei M, et al. 2009. Interleukin-1β-induced extracellular matrix degradation and glycosaminoglycan release is inhibited by curcumin in an explant model of cartilage inflammation. Ann NY Acad Sci 1171: 428-435.
20. Platt D, Bayliss MT,. 1994. An investigation of the proteoglycan metabolism of mature equine articular cartilage and its regulation by interleukin-1. Equine Vet J 26: 297-303.
21. Frisbie DD, Nixon AJ,. 1997. Insulin-like growth factor 1 and corticosteroid modulation of chondrocyte metabolic and mitogenic activities in interleukin 1-conditioned equine cartilage. Am J Vet Res 58: 524-530. (Pubitemid 27253772)
22. Livak KJ, Schmittgen TD,. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-[delta][delta] CT method. Methods 25: 402-408. (Pubitemid 34164012)
23. Ren XD, Schwartz MA,. 2000. Determination of GTP loading on rho. Methods Enzymol 325: 264-272.
24. Taylor SJ, Shalloway D,. 1996. Cell cycle-dependent activation of ras. Curr Biol 6: 1621-1627. (Pubitemid 27050229)
25. Fortier LA, Deak MM, Semevolos SA, et al. 2004. Insulin-like growth factor-I diminishes the activation status and expression of the small GTPase Cdc42 in articular chondrocytes. J Orthop Res 22: 436-445. (Pubitemid 38155303)
26. Flanagan MD, Lin S,. 1980. Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin. J Biol Chem 255: 835.
27. Lancaster CA, Taylor-Harris PM, Self AJ, et al. 1994. Characterization of rhoGAP. A GTPase-activating protein for rho-related small GTPases. J Biol Chem 269: 1137.
28. Barfod E, Zheng Y, Kuang W, et al. 1993. Cloning and expression of a human CDC42 GTPase-activating protein reveals a functional SH3-binding domain. J Biol Chem 268: 26059.
29. Fortier LA, Miller BJ,. 2006. Signaling through the small G-protein Cdc42 is involved in insulin-like growth factor-I resistance in aging articular chondrocytes. J Orthop Res 24: 1765-1772. (Pubitemid 44203308)
30. Wang G, Beier F,. 2005. Rac1/Cdc42 and RhoA GTPases antagonistically regulate chondrocyte proliferation, hypertrophy, and apoptosis. J Bone Miner Res 20: 1022-1031. (Pubitemid 40712827)
31. Liu BP, Burridge K,. 2000. Vav2 activates Rac1, Cdc42, and RhoA downstream from growth factor receptors but not beta 1 integrins. Mol Cell Biol 20: 7160.
32. Porée B, Kypriotou M, Chadjichristos C, et al. 2008. Interleukin-6 (IL-6) and/or soluble IL-6 receptor down-regulation of human type II collagen gene expression in articular chondrocytes requires a decrease of Sp1·Sp3 ratio and of the binding activity of both factors to the COL2A1 promoter. J Biol Chem 283: 4850.
33. Fan Z, Bau B, Yang H, et al. 2004. IL-1 [beta] induction of IL-6 and LIF in normal articular human chondrocytes involves the ERK, p38 and NF [kappa] B signaling pathways. Cytokine 28: 17-24. (Pubitemid 39165616)
34. Nietfeld J, Wilbrink B, Helle M, et al. 1990. Interleukin-1-induced interleukin-6 is required for the inhibition of proteoglycan synthesis by interleukin-1 in human articular cartilage. Arthritis Rheum 33: 1695-1701.
35. Loty S, Forest N, Boulekbache H, et al. 1995. Cytochalasin D induces changes in cell shape and promotes in vitro chondrogenesis: a morphological study. Biol Cell 83: 149-161.
36. Newman P, Watt FM,. 1988. Influence of cytochalasin D-induced changes in cell shape on proteoglycan synthesis by cultured articular chondrocytes. Exp Cell Res 178: 199-210.
37. Kong L, Ge BX,. 2008. MyD88-independent activation of a novel actin-Cdc42/Rac pathway is required for toll-like receptor-stimulated phagocytosis. Cell Res 18: 745-755. (Pubitemid 351948225)
38. Wei L, Imanaka-Yoshida K, Wang L, et al. 2002. Inhibition of rho family GTPases by rho GDP dissociation inhibitor disrupts cardiac morphogenesis and inhibits cardiomyocyte proliferation. Development 129: 1705.