Production of methionine γ-lyase in recombinant Citrobacter freundii bearing the hemoglobin gene

BMB Reports

Volumn 44, Issue 9, 2011, Pages 590-594

  Kahraman, Hüseyin ^a   Aytan, Emel ^a   Kurt, Asli Giray ^a  

^a INONU UNIVERSITY   (Turkey)

Author keywords

Antitumour efficacy; Citrobacter freundii; Methionine lyase; Vitreoscilla hemoglobin

Indexed keywords

BACTERIAL PROTEIN; CARBON; HEMOGLOBIN PROTEIN, VITREOSCILLA; L-METHIONINE GAMMA-LYASE; LYASE; METHIONINE GAMMA LYASE; RECOMBINANT PROTEIN; TRUNCATED HEMOGLOBIN;

ARTICLE; BIOSYNTHESIS; CITROBACTER FREUNDII; ENZYMOLOGY; FERMENTATION; GENETICS; METABOLISM;

BACTERIAL PROTEINS; CARBON; CARBON-SULFUR LYASES; CITROBACTER FREUNDII; FERMENTATION; RECOMBINANT PROTEINS; TRUNCATED HEMOGLOBINS;

EID: 83655192734     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2011.44.9.590     Document Type: Article

References (22)

1. Geckil, H., Gencer, S., Ates, B., Ozer, U., Uckun, M. and Yilmaz, I. (2006) Effect of Vitreoscilla hemoglobin on production of a chemotherapeutic enzyme, L-asparaginase, by Pseudomonas aeruginosa. Biotechnol. J. 1, 203-208.
2. Takakura, T., Ito, T., Yagi, S., Notsu, Y., Itakura, T., Nakamura, T., Inagaki, K., Esaki, N., Hoffman, R. M. and Takimoto, A. (2006) High-level expression and bulk crystallization of recombinant L-methionine γ-lyase, an anticancer agent. Appl. Microbiol. Biotechnol. 70, 183-192.
3. Takakura, T., Mitsushima, K., Yagi, S., Inagaki, K., Tanaka, H., Esaki, N., Soda, K. and Takimotoa, A. (2004) Assay method for antitumor L-methionine γ-lyase: comprehensive kinetic analysis of the complex reaction with L-methionine. Anal. Biochem. 327, 233-240.
4. Weimer, B., Seefeldt, K. and Dias, B. (1999) Sulfur metabolism in bacteria associated with cheese. Anton. Leeuw. 76, 247-261.
5. Manukhov, I. V., Mamaeva, D. V., Morozova, E. A., Rastorguev, S. M., Faleev, N. G., Demidkina, T. V. and Zavilgelsky, G. B. (2006) L-Methionine γ-lyase from Citrobacter freundii: cloning of the gene and kinetic parameters of the enzyme. Biochem. (Moscow) 71, 361-369.
6. Nikulin, A., Revtovich, S., Morozova, E., Nevskaya, N., Nikonov, S., Garber, M. and Demidkina, T. (2008) High-resolution structure of methionine γ-lyase from Citrobacter freundii. Acta. Cryst. 64, 211-218.
7. Kudou, D., Misaki, S., Yamashita, M., Tamura, T., Takakura, T., Yoshioka, T., Yagi, S., Hoffman, R. M., Takimoto, A., Esaki, N. and Inagaki, K. (2007) Structure of the antitumour enzyme L-methionine γ-lyase from Pseudomonas putida at 1.8A° Resolution. J. Biochem. 141, 535-544.
8. Manukhov, I. V., Mamaeva, D. V., Rastorguev, S. M., Faleev, N. G., Morozova, E. A., Demidkina, T. V. and Zavilgelsky, G. B. (2005) A gene encoding L-methionine γ-lyase is present in Enterobacteriaceae family genomes: Identification and characterization of Citrobacter freundii L-methionine γ-lyase. J. Bacteriol. 187, 3889-3893.
9. Mamaeva, D. V., Morozova, E. A., Nikulin, A. D., Revtovich, S. V., Nikonov, S. V., Garberb, M. B. and Demidkinaa, T. V. (2005) Structure of Citrobacter freundii L-methionine γ-lyase. Acta Cryst. Section F 61, 546-549.
10. Yoshimura, M., Nakano, Y. and Koga, T. (2002) L-Methionine-γ-lyase, as a target to inhibit malodorous bacterial growth by trifluoromethionine. Biochem. Bioph. Res. Co. 292, 964-968.
11. Coombs, G. H. and Mottram, J. C. (2001) Trifluoromethionine, a prodrug designed against methionine γ-lyase-containing pathogens, has efficacy in vitro and in vivo against Trichomonas vaginalis. Antimicrob. Agents. Chem. 45, 1743-1745.
12. Kokkinakis, D. M., Schold, S. C., Hori, H. and Nobori, T. (1997) Effect of long-term depletion of plasma methionine on the growth and survival of human brain tumor xenografts in athmic mice. Nutr. Cancer, 29, 195-204.
13. Takakura, T., Takimoto, A., Notsu, Y., Yoshida, H., Ito, T., Nagatome, H., Ohno, M., Kobayashi, Y., Yoshioka, T., Inagaki, K., Yagi, S., Hoffman, R. M. and Esaki, N. (2006) Physicochemical and pharmacokinetic characterization of highly potent recombinant L-methionine γ-lyase conjugated with polyethylene glycol as an antitumor agent. Cancer Res. 66, 2807-2814.
14. Lishko, V. K., Lishko, O. V. and Hoffman, R. M. (1993) Depletion of serum methionine by methioninase in mice. Anticancer Res. 13, 1465-1468.
15. Borenshtein, D. and Schauer, D. B. (2006) The genus Citrobacter. Prokaryotes 6, 90-98.
16. Wang, J., Chang, S., Chen, Y. and Luh, K. J. (2000) Comparison of antimicrobial susceptibility of Citrobacter freundii isolates in two different time periods. Microbiol. Immunol. Infect. 33, 258-262.
17. Geckil, H., Gencer, S., Kahraman, H. and Erenler, S. O. (2003) Genetic engineering of Enterobacter aerogenes with Vitreoscilla hemoglobin gene: cell growth, survival, and antioxidant enzyme status under oxidative stress. Res. Microbiol. 154, 425-431.
18. Geckil, H., Stark, B. C. and Webster, D. A. (2001) Cell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently affected by the genetically engineered Vitreoscilla hemoglobin gene. J. Biotechnol. 85, 57-66.
19. Yang, J., Webster, D. A. and Stark, B. C. (2005) ArcA Works with Fnr as a positive regulator of Vitreoscilla (bacterial) hemoglobin gene expression in Escherichia coli. Microbiol. Res. 160, 405-415.
20. Kurt, A. G., Aytan, E., Ozer, U., Ates, B. and Geckil, H. (2009) Production of L- DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene. Biotechnol. J. 4, 1077-1088.
21. Tanaka, H., Imahara, H., Esaki, N. and Soda, K. (1980) Selective determination of L-methionine and L-cysteine with bacterial L-methionine γ-lyase and anti-tumor activity of the enzyme. J. Appl. Biochem. 2, 439-444.
22. Soda, K. (1968) Microdetermination of D-amino acids and D-amino acid oxidase activity with 3-methyl-2-benzothiazolone hydrazone hydrochloride. Anal. Biochem. 25, 228-235.