Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria

Journal of Bacteriology

Volumn 178, Issue 4, 1996, Pages 1197-1199

  Pelicic, Vladimir ^a   Reyrat, Jean Marc ^a   Gicquel, Brigitte ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

LEVANSUCRASE; SUCROSE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL METABOLISM; ENZYME ACTIVITY; GENE EXPRESSION; MYCOBACTERIUM BOVIS; MYCOBACTERIUM SMEGMATIS; NONHUMAN; PRIORITY JOURNAL;

EID: 0030030736     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.4.1197-1199.1996     Document Type: Article

References (22)

1. Baulard, A., C. Jourdan, A. Mercenier, and C. Locht. 1992. Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli Nucleic Acids Res. 20:4105.
2. Beveridge, T. J. 1995. The periplasmic space and the periplasm in gram-positive and gram-negative bacteria ASM News 61:125-130.
3. Blomfield, I. C., V. Vaughn, R. F. Rest, and B. I. Eisenstein. 1991. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature sensitive pSC101 replicon. Mol. Microbiol. 5:1447-1457.
4. Cianciotto, N. P., R. Long, B. I. Einsenstein, and N. C. Engleberg. 1988. Site-specific mutagenesis in Legtonella pneumophila by allelic exchange using counterselectable colE1 vectors. FEMS Microbiol. Lett. 56:203-208.
5. Dedonder, R. 1966. Levansucrase from Bacillus subtilis. Methods Enzymol. 8:500-505.
6. Gay, P., D. Le Coq, M. Steinmetz, T. Berkelman, and C. I. Kado. 1985. Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 164:918-921.
7. Guilhot, C., B. Gicquel, and C. Martin. 1992. Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000. FEMS Microbiol. Lett. 98:181-186.
8. Jacobs, W. R. 1992. Advances in mycobacterial genetics: new promises for old diseases. Immunobiology 184:147-156.
9. Jäger, W., A. Schäfer, A. Pühler, G. Labes, and W. Wohlieben. 1992, Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J. Bacteriol. 174:5462-5465.
10. Kalpana, G. V., B. R. Bloom, and W. R. Jacobs, Jr. 1991. Insertional mutagenesis and illegitimate recombination in mycobacteria. Proc. Natl Acad. Sci. USA 88:5433-5437.
11. Lepesant, J. A., F. Kunst, J. Lepesant-Kejzlarovà, and R. Dedonder. 1972. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg. Mol. Gen. Genet. 118:135-160.
12. Quandt, J., and M. F. Hynes. 1993. Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127: 15-21.
13. Ranes, M. G., J. Rauzier, M. Lagranderie, M. Gheorghiu, and B. Gicquel. 1990. Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a "mini" mycobacterium-Escherichia coli shuttle vector. J. Bacteriol. 172:2793-2797.
14. Rastogi, N. 1991. Structure and functions of the cell envelope in relation to mycobacterial virulence, pathogenicity and multiple drug resistance 7th Forum in Microbiology. Res. Microbiol. 142:419-481.
15. Reyrat, J. M., F. X. Berthet, and B. Gicquel. 1995. The urease locus of Mycobacterium tuberculosis and its utilization for the demonstration of allelic exchange in Mycobacterium bovis bacillus Calmette-Guérin. Proc. Natl. Acad. Sci. USA 92:8768-8772.
16. Ried, J. L., and A. Collmer. 1987. An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in Gram-negative bacteria by marker exchange-eviction mutagenesis. Gene 57:239-246.
17. Sander, P., A. Meier, and E. C. Bottger. 1995. rpsL+ , a dominant selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16:991-1000.
18. Snapper, S. B., R. E. Melton, S. Mustapha, T. Kieser, and W. R. Jacobs. 1990. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4:1911-1919.
19. Soupène, E., M. Foussard, P. Boistard, G. Truchet, and J. Batut. 1995. Oxygen as a key developmental regulator of Rhizobium meliloti N2-fixation gene expression within the alfalfa root nodule. Proc. Natl. Acad. Sci. USA 92:3759-3763.
20. Steinmetz, M., D. Le Coq, H. B. Djemia, and P. Gay. 1983. Analyse génétique de sacB, gène de structure d'une enzyme sécrétée, la lévane-saccharase de Bacillus subtilis Marburg Mol. Gen. Genet. 191:138-144.
21. Stibitz, S. 1994. Use of conditionally counterselectable suicide vectors for allelic exchange. Methods Enzymol. 235:458-465.
22. Wayne, L. G., and G. P. Kubica. 1986. Mycobacteria, p. 1435-1457. In P A. Sneath (ed.), Bergey's manual of systematic bacteriology. Williams & Wilkins, Baltimore.