High-level expression and purification of human epidermal growth factor with SUMO fusion in Escherichia coli

Protein and Peptide Letters

Volumn 13, Issue 8, 2006, Pages 785-792

  Su, Zhijian ^a,b   Huang, Yadong ^b   Zhou, Quannan ^b   Wu, Zhiling ^b   Wu, Xiaoping ^b   Zheng, Qing ^b   Ding, Changcai ^b   Li, Xiaokun ^b  

^a SUN YAT SEN UNIVERSITY   (China)

^b JINAN UNIVERSITY   (China)

Author keywords

Escherichia coli; Human epidermal growth factor; Protein expression; SUMO fusion

Indexed keywords

EPIDERMAL GROWTH FACTOR; HYBRID PROTEIN; SUMO PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CELL STRAIN 3T3; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MITOSIS; MOUSE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANIMALS; BALB 3T3 CELLS; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPIDERMAL GROWTH FACTOR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; MICE; MITOSIS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33746651527     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986606777841280     Document Type: Article

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