Assessment of GFP expression and viability using the Tali image-based cytometer

Journal of Visualized Experiments

Volumn , Issue 57, 2011, Pages

  Remple, Krissy ^a   Stone, Laurel ^a  

^a Life Technologies   (United States)

Author keywords

Apoptosis; Cell analysis; Cell biology; Cell counting; Cell viability; Cytometry; Dead cell red; Expression assay; Fluorescence protein expression; GFP expression; Image based; Imaging; Issue 57; PI; Propidium iodide; RFP expression

Indexed keywords

EID: 83455195294     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3659     Document Type: Article

References (12)

1. Godfrey, W.L., Hill, D.M., Kilgore, J.A., Buller, G.M., Bradford, J.A., Gray, D.R., Clements, I., Oakleaf, K., Salisbury, J.J., Ignatius, M.J., & Janes, M.S. Complementarity of Flow Cytometry and Fluorescence Microscopy. Microsc. Microanal. 11, 246-247 (2005).
2. Mittag, A., Pinto, F.E., Endringer, D.C., Tarnok, A., & Lenz, D. Cellular analysis by open-source software for affordable cytometry. Scanning. 33, 33-40 (2011).
3. Patterson, G.H., Knobel, S.M., Sharif, W.D., Kain, S.R. & Piston, D.W. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys. J. 73, 2782-90 (1997).
4. Smyth, P.G. & Berman, S.A. Markers of apoptosis: methods for elucidating the mechanism of apoptotic cell death from the nervous system. Biotechniques. 32, 648-50, 652, 654 passim (2002).
5. Vermes, I., Haanen, C., Steffens-Nakken, H., & Reutelingsperger, C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods. 184, 39-51 (1995).
6. Koopman, G., et al. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 84, 1415-20 (1994).
7. Tanke, H.J., van der Linden, P.W., & Langerak, J. Alternative fluorochromes to ethidium bromide for automated read out of cytotoxicity tests. J. Immunol. Methods. 52, 91-6 (1982).
8. King, M.A. Detection of dead cells and measurement of cell killing by flow cytometry. J. Immunol. Methods. 243, 155-66 (2000).
9. Jones, K.H. & Kniss, D.A. Propidium iodide as a nuclear counterstain for immunofluorescence studies on cells in culture. J. Histochem. Cytochem. 35, 123-5 (1987).
10. Dell'Arciprete, R., et al. High-efficiency expression gene cloning by flow cytometry. J. Histochem. Cytochem. 44, 629-40 (1996).
11. Lamm, G.M., Steinlein, P., Cotten, M., & Christofori, G. A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells. Nucleic. Acids. Res. 25, 4855-7 (1997).
12. Perfetto, S.P., et al. Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. J. Immunol. Methods. 313, 199-208 (2006).