A long-term siRNA strategy regulates fibronectin overexpression and improves vascular lesions in retinas of diabetic rats

Molecular Vision

Volumn 17, Issue , 2011, Pages 3166-3174

  Roy, Sumon ^a   Nasser, Sigrid ^a   Yee, Melissa ^a   Graves, Dana T ^b   Roy, Sayon ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PENNSYLVANIA SCHOOL OF DENTAL MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FIBRONECTIN; SMALL INTERFERING RNA; STREPTOZOCIN; TRYPSIN;

ACELLULAR CAPILLARY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BASEMENT MEMBRANE; CAPILLARY; CAPILLARY BASEMENT MEMBRANE THICKENING; CELL LOSS; CONTROLLED STUDY; DIABETES MELLITUS; DIABETIC RETINOPATHY; DISEASE MODEL; DOSE RESPONSE; DOWN REGULATION; DRUG EFFICACY; DRUG SAFETY; DRUG TOLERABILITY; ELECTRON MICROSCOPY; GENE OVEREXPRESSION; IN VIVO STUDY; MALE; MICROANGIOPATHY; NONHUMAN; PERICYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TARGETING; RAT; RETINA; RETINA CELL; WESTERN BLOTTING;

ANIMALS; BASEMENT MEMBRANE; BLOOD GLUCOSE; BODY WEIGHT; CAPILLARIES; DIABETIC RETINOPATHY; FIBRONECTINS; HEMOGLOBIN A, GLYCOSYLATED; INTRAVITREAL INJECTIONS; MALE; PERICYTES; RATS; RATS, SPRAGUE-DAWLEY; RETINA; RNA, SMALL INTERFERING; TIME FACTORS;

EID: 83055178335     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Oshitari T, Polewski P, Chadda M, Li AF, Sato T, Roy S. Effect of combined antisense oligonucleotides against high-glucoseand diabetes-induced overexpression of extracellular matrix components and increased vascular permeability. Diabetes 2006; 55:86-92. [PMID: 16380480]
2. Evans T, Deng DX, Chen S, Chakrabarti S. Endothelin receptor blockade prevents augmented extracellular matrix component mRNA expression and capillary basement membrane thickening in the retina of diabetic and galactose-fed rats. Diabetes 2000; 49:662-6. [PMID: 10871206]
3. Fukui M. Altered glomerular mRNA expression of basement membrane components and type I collagen in diabetic rats treated with or without insulin therapy. Nippon Jinzo Gakkai Shi 1991; 33:275-81. [PMID: 2062013]
4. Seon YD, Lee TH, Lee MC. Changes of glomerular basement membrane components in Vacor-induced diabetic nephropathy. Korean J Intern Med 1999; 14:77-84. [PMID: 10063318]
5. Li AF, Sato T, Haimovici R, Okamoto T, Roy S. High glucose alters connexin 43 expression and gap junction intercellular communication activity in retinal pericytes. Invest Ophthalmol Vis Sci 2003; 44:5376-82. [PMID: 14638741]
6. Roy S, Sato T, Paryani G, Kao R. Downregulation of fibronectin overexpression reduces basement membrane thickening and vascular lesions in retinas of galactose-fed rats. Diabetes 2003; 52:1229-34. [PMID: 12716757]
7. Cherian S, Roy S, Pinheiro A. Tight glycemic control regulates fibronectin expression and basement membrane thickening in retinal and glomerular capillaries of diabetic rats. Invest Ophthalmol Vis Sci 2009; 50:943-9. [PMID: 18775856]
8. Bertrand JR, Pottier M, Vekris A, Opolon P, Maksimenko A, Malvy C. Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo. Biochem Biophys Res Commun 2002; 296:1000-4. [PMID: 12200148]
9. Oshitari T, Brown D, Roy S. SiRNA strategy against overexpression of extracellular matrix in diabetic retinopathy. Exp Eye Res 2005; 81:32-7. [PMID: 15978252]
10. Roy S, Cagliero E, Lorenzi M. Fibronectin overexpression in retinal microvessels of patients with diabetes. Invest Ophthalmol Vis Sci 1996; 37:258-66. [PMID: 8603829]
11. Roy S, Zhang K, Roth T, Vinogradov S, Kao RS, Kabanov A. Reduction of fibronectin expression by intravitreal administration of antisense oligonucleotides. Nat Biotechnol 1999; 17:476-9. [PMID: 10331808]
12. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411:494-8. [PMID: 11373684]
13. Siperstein MD, Norton W, Unger RH, Madison LL. Muscle capillary basement membrane width in normal, diabetic and prediabetic patients. Trans Assoc Am Physicians 1966; 79:330-47. [PMID: 5929467]
14. Kuwabara T, Cogan D. Studies of retinal vascular patterns. Part I. Normal architecture. Arch Ophthalmol 1960; 64:904-11. [PMID: 13755464]
15. Dhar S, Roy S, Cherian S, Roy S. High Glucose-Induced Altered Extracellular Matrix Triggers Apoptosis in Meicrovascular Endothelial Cells. ARVO Annual Meeting; 2006 April 30-May 4; Fort Lauderdale (FL).
16. Das A, Frank RN, Zhang NL, Samadani E. Increases in collagen type IV and laminin in galactose-induced retinal capillary basement membrane thickening-prevention by an aldose reductase inhibitor. Exp Eye Res 1990; 50:269-80. [PMID: 2108050]
17. Das A, Frank RN, Zhang NL, Turczyn TJ. Ultrastructural localization of extracellular matrix components in human retinal vessels and Bruch's membrane. Arch Ophthalmol 1990; 108:421-9. [PMID: 2310346]
18. Mandarino LJ, Sundarraj N, Finlayson J, Hassell HR. Regulation of fibronectin and laminin synthesis by retinal capillary endothelial cells and pericytes in vitro. Exp Eye Res 1993; 57:609-21. [PMID: 8282048]
19. Osterby R. Basement membrane morphology in diabetes mellitus. In: Rifkin H, Porte DJ, editors. In Diabetes Mellitus Theory and Practice. 4th ed. New York: Elsevier Science; 1990. p. 220-33.
20. Roy S, Maiello M, Lorenzi M. Increased expression of basement membrane collagen in human diabetic retinopathy. J Clin Invest 1994; 93:438-42. [PMID: 8282817]
21. Roy S, Paryani G, Kao RS. Antisense oligonucleotides modulate high glucose-induced laminin overexpression and cell proliferation. Diabetes 1999; 48:A113.
22. Roy S, Roth T. Proliferative effect of high glucose is modulated by antisense oligonucleotides against fibronectin in rat endothelial cells. Diabetologia 1997; 40:1011-7. [PMID: 9300237]
23. Roy S, Rothschild JG, Chen A. Vascular complications and gene therapy. Expert Opin Biol Ther 2003; 3:71-83. [PMID: 12718732]
24. Roy S, Sato T, Kao RS, Paryani G. Effect of reduced fibronectin expression in retinal capillary Basement membranes of galactose-fed rats. Diabetes 2000; 49:A123.
25. Behl Y, Krothapalli P, Desta T, Roy S, Graves DT. FOXO1 plays an important role in enhanced microvascular cell apoptosis and microvascular cell loss in type 1 and type 2 diabetic rats. Diabetes 2009; 58:917-25. [PMID: 19168598]
26. Alikhani M, Roy S, Graves DT. FOXO1 plays an essential role in apoptosis of retinal pericytes. Mol Vis 2010; 16:408-15. [PMID: 20300563]
27. Behl Y, Krothapalli P, Desta T, DiPiazza A, Roy S, Graves DT. Diabetes-enhanced tumor necrosis factor-alpha production promotes apoptosis and the loss of retinal microvascular cells in type 1 and type 2 models of diabetic retinopathy. Am J Pathol 2008; 172:1411-8. [PMID: 18403591]
28. Kim DH, Behlke MA, Rose SD, Chang MS, Choi S, Rossi JJ. Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nat Biotechnol 2005; 23:222-6. [PMID: 15619617]