Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development

PLoS ONE

Volumn 6, Issue 12, 2011, Pages

  Wilke, Sonja ^a   Groebe, Lothar ^a   Maffenbeier, Vitali ^a   Jäger, Volker ^a   Gossen, Manfred ^b,c   Josewski, Jörn ^a   Duda, Agathe ^a   Polle, Lilia ^a   Owens, Raymond J ^d,e   Wirth, Dagmar ^a   Heinz, Dirk W ^a   van den Heuvel, Joop ^a   Büssow, Konrad ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c Berlin Brandenburg Centre for Regenerative Therapies (BCRT)   (Germany)

^d UNIVERSITY OF OXFORD   (United Kingdom)

^e RUTHERFORD APPLETON LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN; DNA POLYMERASE; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN; RECOMBINASE; RED FLUORESCENT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL LINE; CELL MATURATION; CELL MUTANT; CELL SELECTION; CHO CELL; CONTROLLED STUDY; CRYSTALLIZATION; DEGLYCOSYLATION; GENETIC ENGINEERING; GENETIC TRANSFECTION; GLYCOSYLATION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; TARGET CELL; VALIDATION STUDY; ANIMAL; BIOPHYSICS; BIOSYNTHESIS; CHEMISTRY; CRICETULUS; CULTURE TECHNIQUE; CYTOLOGY; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; HAMSTER; METABOLISM; METHODOLOGY; PROTEIN TERTIARY STRUCTURE;

ANIMALS; BIOPHYSICAL PHENOMENA; CELL CULTURE TECHNIQUES; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; CRYSTALLIZATION; DNA NUCLEOTIDYLTRANSFERASES; GENETIC VECTORS; GLYCOPROTEINS; GLYCOSYLATION; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC;

EID: 83055168492     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027829     Document Type: Article

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