Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting

Protein Science

Volumn 19, Issue 6, 2010, Pages 1264-1271

  Wilke, Sonja ^a   Krausze, Joern ^a   Gossen, Manfred ^b,c   Groebe, Lothar ^a   Jäger, Volker ^a   Gherardi, Ermanno ^d   Van Den Heuvel, Joop ^a   Büssow, Konrad ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c Berlin Brandenburg Center for Regenerative Therapies   (Germany)

^d MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

CD208; Chinese hamster ovary cells; CHO; Crystallization; DC LAMP; Glycoprotein; Hepatocyte growth factor; HGF; LAMP 3; Scatter factor

Indexed keywords

GLYCOPROTEIN; GREEN FLUORESCENT PROTEIN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 3; RECOMBINASE; SCATTER FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL MUTANT; CHO CELL; CONTROLLED STUDY; CRYSTALLIZATION; DEGLYCOSYLATION; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GENE CONSTRUCT; GENETIC ENGINEERING; GENETIC TRANSFECTION; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

ANIMALS; CELL ADHESION MOLECULES, NEURONAL; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; GLYCOSYLATION; GREEN FLUORESCENT PROTEINS; HEPATOCYTE GROWTH FACTOR; HUMANS; PROTO-ONCOGENE PROTEINS; RECOMBINANT FUSION PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 77952726323     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.390     Document Type: Article

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