Application of the modular approach to an in-house validation study of real-time PCR methods for the detection and serogroup determination of verocytotoxigenic Escherichia coli

Applied and Environmental Microbiology

Volumn 77, Issue 19, 2011, Pages 6954-6963

  Kagkli, Dafni Maria ^a   Weber, Thomas P ^a   Van den Bulcke, Marc ^a   Folloni, Silvia ^a   Tozzoli, Rosangela ^b   Morabito, Stefano ^b   Ermolli, Monica ^a   Gribaldo, Laura ^a   Van den Eede, Guy ^a  

^a JOINT RESEARCH CENTRE   (Italy)

^b ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTICAL PROCESS; BIOLOGICAL HAZARD; CAUSATIVE AGENTS; CRITICAL PARAMETER; DYNAMIC RANGE; E. COLI; EUROPEAN COMMISSION; EUROPEAN FOOD SAFETY AUTHORITIES; FOOD MATRIXES; FOOD MICROBIOLOGY; HYGIENIC CONDITIONS; INTIMIN; LIMIT OF DETECTION; MICROBIOLOGICAL CRITERIA; MODULAR APPROACH; PCR EFFICIENCY; PCR METHOD; PERFORMANCE CRITERION; REAL-TIME PCR; REAL-TIME PCR METHOD; SEROGROUPS; TECHNICAL SPECIFICATIONS; TOXIN GENES; VALIDATION STUDY;

ESCHERICHIA COLI; GENES; POLYMERASE CHAIN REACTION;

FOOD SAFETY;

COLIFORM BACTERIUM; EUROPEAN COMMISSION; POLYMERASE CHAIN REACTION; REAL TIME; TOXICITY;

ARTICLE; CLASSIFICATION; ESCHERICHIA COLI INFECTION; FOOD CONTROL; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; VALIDATION STUDY;

BACTERIOLOGICAL TECHNIQUES; ESCHERICHIA COLI INFECTIONS; FOOD MICROBIOLOGY; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 82955234155     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05357-11     Document Type: Article

References (24)

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