Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction

Journal of Applied Microbiology

Volumn 98, Issue 5, 2005, Pages 1162-1168

  Perelle, S ^a   Dilasser, F ^a   Grout, J ^a   Fach, Patrick ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

Author keywords

Escherichia coli O103; O antigen gene cluster; Real time PCR; Shiga toxin producing E. coli

Indexed keywords

ANTIGENS; ASSAYS; BIOCHEMISTRY; GENES; POLYMERASE CHAIN REACTION;

E. COLI; ESCHERICHIA COLI O103; O-ANTIGEN; O-ANTIGEN GENE CLUSTER; REAL-TIME PCR; REAL-TIME PCR ASSAY; SEROGROUPS; SEROTYPING; SHIGA TOXIN-PRODUCING E. COLI; SHIGA TOXIN-PRODUCING ESCHERICHIA COLI;

ESCHERICHIA COLI;

FLICE INHIBITORY PROTEIN; FLIPPASE; O ANTIGEN; PROTEINASE; SHIGA TOXIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

GENE; IDENTIFICATION METHOD; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; DNA FLANKING REGION; DNA LIBRARY; DNA PROBE; ESCHERICHIA COLI; ESCHERICHIA COLI O103; GALE GENE; GENE AMPLIFICATION; GENE CLUSTER; GND GENE; JUMPSTART GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SEROTYPING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; WZX GENE;

ESCHERICHIA COLI; GALE;

EID: 17644374069     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2005.02545.x     Document Type: Article

References (37)

1. Cebula, T.A., Payne, W.L. and Feng, P. (1995) Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. Journal of Clinical Microbiology 33, 248-250. Published erratum appears in Journal of Clinical Microbiology 33, 1048.
2. Cebula, T.A., Payne, W.L. and Feng, P. (1995) Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. Journal of Clinical Microbiology 33, 248-250. Published erratum appears in Journal of Clinical Microbiology 33, 1048.
3. Coimbra, R.S., Grimont, F. and Grimont, P.A. (1999) Identification of Shigella serotypes by restriction of amplified O-antigen gene cluster. Research in Microbiology 150, 543-553.
4. Coimbra, R.S., Grimont, F., Lenormand, P., Burguiere, P., Beutin, L. and Grimont, P.A. (2000) Identification of Escherichia coli O-serogroups by restriction of the amplified O-antigen gene cluster (rfb-RFLP). Research in Microbiology 151, 639-654.
5. D'Souza, J.M., Wang, L. and Reeves, P. (2002) Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes. Gene 297, 123-127.
6. DebRoy, C., Roberts, E., Kundrat, J., Davis, M.A., Briggs, C.E. and Fratamico, P.M. (2004) Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes. Applied and Environmental Microbiology 70, 1830-1832.
7. Desmarchelier, P.M., Bilge, S.S., Fegan, N., Mills, L., Vary, J.C., Jr and Tarr, P.I. (1998) A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. Journal of Clinical Microbiology 36, 1801-1804.
8. Fach, P., Perelle, S., Dilasser, F. and Grout, J. (2001) Comparison between a PCR-ELISA test and the Vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains. Journal of Applied Microbiology 90, 809-818.
9. Feng, L., Wang, W., Tao, J., Guo, H., Krause, G., Beutin, L. and Wang, L. (2004) Identification of Escherichia coli O114 O-antigen gene cluster and development of an O114 serogroup-specific PCR assay. Journal of Clinical Microbiology 42, 3799-3804.
10. Fratamico, P.M., Briggs, C.E., Needle, D., Chen, C.Y. and DebRoy, C. (2003) Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes. Journal of Clinical Microbiology 41, 3379-3383.
11. Gannon, V.P.J., Rashed, M., King, R.K. and Golsteyn-Thomas, E.J. (1993) Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction. Journal of Clinical Microbiology 31, 1268-1274.
12. Guo, H., Feng, L., Tao, J., Zhang, C. and Wang, L. (2004) Identification of Escherichia coli O172 O-antigen gene cluster and development of a serogroup-specific PCR assay. Journal of Applied Microbiology 97, 181-190.
13. Hobbs, M. and Reeves, P.R. (1994) The JUMPstart sequence: a 39 bp element common to several polysaccharide gene clusters. Molecular Microbiology 12, 855-856.
14. Karmali, M.A. (1989) Infection by verocytotoxin-producing Escherichia coli. Clinical Microbiology Reviews 2, 15-38.
15. Louie, M., De Azavedo, J., Clarke, R., Borczyk, A., Lior, H., Richter, M. and Brunton, J. (1994) Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers. Epidemiology and Infection 112, 449-461.
16. Louie, M., Read, S., Simor, A.E., Holland, J., Louie, L., Ziebell, K., Brunton, J. and Hii, J. (1998) Application of multiplex PCR for detection of non-O157 verocytotoxin-producing Escherichia coli in bloody stools: identification of serogroups O26 and O111. Journal of Clinical Microbiology 36, 3375-3377.
17. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
18. Maurer, J.J., Schmidt, D., Petrosko, P., Sanchez, S., Bolton, L. and Lee, M.D. (1999) Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Applied and Environmental Microbiology 65, 2954-2960.
19. Nataro, J.P. and Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clinical Microbiology Reviews 11, 142-201. Published erratum appears in Clinical Microbiology Reviews 11, 403.
20. Nataro, J.P. and Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clinical Microbiology Reviews 11, 142-201. Published erratum appears in Clinical Microbiology Reviews 11, 403.
21. Oswald, E., Schmidt, H., Morabito, S., Karch, H., Marchès, O. and Caprioli, A. (2000) Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infection and Immunity 68, 64-71.
22. Paton, J.C. and Paton, A.W. (1998a) Pathogenesis and diagnosis of Shiga Toxin-producing Escherichia coli infections. Clinical Microbiology Reviews 11, 450-479.
23. O157. Journal of Clinical Microbiology 36, 598-602.
24. Paton, A.W. and Paton, J.C. (1999a) Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113. Infection and Immunity 67, 5930-5937.
25. Paton, A.W. and Paton, J.C. (1999b) Direct detection of Shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157, and O113 by multiplex PCR. Journal of Clinical Microbiology 37, 3362-3365.
26. O157). Journal of Applied Microbiology 93, 250-260.
27. Perelle, S., Dilasser, F., Grout, J. and Fach, P. (2002b) Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test. Journal of Applied Microbiology 93, 758-764.
28. Perelle, S., Dilasser, F., Grout, J. and Fach, P. (2003) Development of a 5′-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an O-island 29' homologue. Journal of Applied Microbiology 94, 587-594.
29. Perelle, S., Dilasser, F., Grout, J. and Fach, P. (2004) Detection by 5′-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world's most frequent clinical cases. Molecular and Cellular Probe; 18, 185-192.
30. Russmann, H., Kothe, E., Schmidt, H., Franke, S., Harmsen, D., Caprioli, A. and Karch, H. (1995) Genotyping of Shiga-like toxin genes in non-O157 Escherichia coli strains associated with haemolytic uraemic syndrome. Journal of Medical Microbiology 42, 404-410.
31. Sharma, V.K. (2002) Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction. Journal of Food Protection 65, 1371-1380.
32. Sharma, V.K. and Dean-Nystrom, E.A. (2003) Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Veterinary Microbiology 93, 247-260.
33. Wang, L. and Reeves, P.R. (1998) Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes. Infection and Immunity 66, 3545-3551.
34. Wang, L., Curd, H., Qu, W. and Reeves, P.R. (1998) Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes. Journal of Clinical Microbiology 36, 3182-3187.
35. Wang, L., Briggs, C.E., Rothemund, D., Fratamico, P., Luchansky, J.B. and Reeves, P.R. (2001) Sequence of the E. coli O104 antigen gene cluster and identification of O104 specific genes. Gene 30, 231-236.
36. Wang, L., Huskic, S., Cisterne, A., Rothemund, D. and Reeves, P.R. (2002) The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene. Journal of Bacteriology 184, 2620-2625.
37. Yoshitomi, K.J., Jinneman, K.C. and Weagant, S.D. (2003) Optimization of a 3′-minor groove binder-DNA probe targeting the uidA gene for rapid identification of Escherichia coli O157:H7 using real-time PCR. Molecular and Cellular Probes 17, 275-280.