Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 879, Issue 32, 2011, Pages 3853-3858

  Barbosa, José Murillo P ^a   Souza, Ranyere L ^a   Fricks, Alini T ^a,b   Zanin, Gisella Maria ^c   Soares, Cleide Mara F ^a,b   Lima, Álvaro S ^a,b  

^a TIRADENTES UNIVERSITY   (Brazil)

^b ITP   (Brazil)

^c STATE UNIVERSITY OF MARINGÁ   (Brazil)

Author keywords

Aqueous two phase system; Lipolytic enzyme; Purification

Indexed keywords

AMMONIUM SULPHATE; AQUEOUS TWO PHASE SYSTEM; BACILLUS SP; ENZYME PURIFICATION; LIPOLYTIC ENZYMES; NEW SOURCES; PH VALUE; POTASSIUM PHOSPHATE; PROTEIN CONTENTS; PURIFICATION FACTORS; PURIFIED ENZYME; SDS-PAGE; SUBMERGED FERMENTATION; ULTRA-PURE WATER;

AMMONIUM COMPOUNDS; BACILLI; BACTERIOLOGY; GLYCOLS; LIPASES; PHOSPHATES; POLYETHYLENE GLYCOLS; POTASSIUM; SODIUM CHLORIDE;

PURIFICATION;

AMMONIUM SULFATE; MACROGOL; POTASSIUM DIHYDROGEN PHOSPHATE; SODIUM CHLORIDE; TRIACYLGLYCEROL LIPASE; WATER;

AQUEOUS SOLUTION; AQUEOUS TWO PHASE SYSTEM; ARTICLE; BACILLUS; ENTROPY; ENZYME PURIFICATION; HYDROPHOBICITY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; VISCOSITY;

BACILLUS; CHEMICAL FRACTIONATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LIPASE; PHOSPHATES; POLYETHYLENE GLYCOLS; POTASSIUM COMPOUNDS; SODIUM CHLORIDE; TEMPERATURE;

BACILLUS SP.;

EID: 82955233644     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2011.10.035     Document Type: Article

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