S-layer fusion proteins-construction principles and applications

Current Opinion in Biotechnology

Volumn 22, Issue 6, 2011, Pages 824-831

  Ilk, Nicola ^a   Egelseer, Eva M ^a   Sleytr, Uwe B ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ARCHAEA; BACTERIAL CELL SURFACE; BROAD SPECTRUM; CELL ENVELOPES; DIAGNOSTIC DEVICE; ENCAPSULATION SYSTEMS; ENGINEERING TECHNIQUES; ENZYME MEMBRANES; FUNCTIONAL DOMAINS; MICROCARRIERS; S-LAYER PROTEINS; S-LAYERS; WEALTH OF INFORMATION;

BACTERIA; BIOMIMETICS; BIOSENSORS; CELL MEMBRANES; CYTOLOGY; GENETIC ENGINEERING;

PROTEINS;

ALLERGY VACCINE; ANTHRAX VACCINE; ANTIALLERGIC AGENT; BACTERIAL PROTEIN; EPITOPE; HAEMOPHILUS INFLUENZAE VACCINE; HYBRID PROTEIN; LIVE VACCINE; PROSTATE SPECIFIC ANTIGEN; RECOMBINANT PROTEIN; S LAYER PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; BACTERIAL MEMBRANE; BINDING AFFINITY; BIOCATALYST; BIOMIMETICS; BIOSENSOR; CARBOXY TERMINAL SEQUENCE; CHIMERA; CROSS REACTION; CRYSTALLINE BACTERIAL CELL SURFACE LAYER; CRYSTALLIZATION; DNA MICROARRAY; DRUG DELIVERY SYSTEM; DRUG DESIGN; HUMAN; IMMUNOASSAY; IMMUNOGENICITY; NANOBIOTECHNOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN IMMOBILIZATION; PROTEIN MICROARRAY; REVIEW; SENSITIVITY AND SPECIFICITY; SOLID PHASE IMMUNOASSAY; STRUCTURE ANALYSIS; SURFACE PLASMON RESONANCE;

ARCHAEA; BACTERIA; BIODEGRADATION, ENVIRONMENTAL; BIOLOGICAL MARKERS; BIOSENSING TECHNIQUES; ENZYMES; GLYCOPROTEINS; MEMBRANE GLYCOPROTEINS; NANOPARTICLES; RECOMBINANT PROTEINS; VACCINES;

BACTERIA (MICROORGANISMS);

EID: 82755182961     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2011.05.510     Document Type: Review

References (53)

1. Crystalline Bacterial Cell Surface Layers 1988, Springer, Berlin. U.B. Sleytr, P. Messner, D. Pum, Sára M (Eds.).
2. Messner P., Schäffer C., Egelseer E.M., Sleytr U.B. Occurrence, structure, chemistry, genetics, morphogenesis, and function of S-layers. Prokaryotic Cell Wall Compounds-Structure and Biochemistry 2010, 53-109. Springer-Verlag. H. König, H. Claus, A. Varma (Eds.).
3. Sleytr U.B., Sára M., Pum D., Schuster B. Crystalline bacterial cell surface layers (S-layers): a versatile self-assembly system. Supramolecular Polymers 2005, 583-612. edn 2. A. Ciferri (Ed.).
4. Horejs C., Pum D., Sleytr U.B., Peterlik H., Jungbauer A., Tscheliessnig R. Surface layer protein characterization by small angle X-ray scattering and a fractal mean force concept: from protein structure to nanodisk assemblies. J Chem Phys 2010, 133:175102.
5. Horejs C., Pum D., Sleytr U.B., Tscheliessnig R. Structure prediction of an S-layer protein by the mean force method. J Chem Phys 2008, 128. 065106 (065101-065111).
6. Sleytr U.B., Egelseer E.M., Ilk N., Messner P., Schäffer C., Pum D., Schuster B. Nanobiotechnological applications of S-layers. Prokaryotic Cell Wall Compounds-Structure and Biochemistry 2010, 459-481. Springer. H. König, H. Claus, A. Varma (Eds.).
7. Sleytr U.B., Sára M., Pum D., Schuster B. Characterization and use of crystalline bacterial cell surface layers. Prog Surf Sci 2001, 68:231-278.
8. Sleytr U.B., Sára M., Pum D., Schuster B. Molecular nanotechnology and nanobiotechnology with two-dimensional protein crystals (S-layers). Nano-Surface Chemistry 2001, 333-389. Marcel Dekker Inc. M. Rosoff (Ed.).
9. Sleytr U.B., Sára M., Pum D., Schuster B., Messner P., Schäffer C. Self-assembly protein systems: microbial S-layers. Biopolymers 2002, vol 1st Ed.:285-338. Wiley-VCH. A. Steinbüchel, S.R. Fahnestock (Eds.).
10. Egelseer E.M., Ilk N., Pum D., Messner P., Schäffer C., Schuster B., Sleytr U.B. S-layers, microbial, biotechnological applications. The Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology 2010, vol 7:4424-4448. John Wiley & Sons Inc.
11. Sára M., Pum D., Schuster B., Sleytr U.B. S-layers as patterning elements for application in nanobiotechnology. J Nanosci Nanotechnol 2005, 5:1939-1953.
12. Sleytr U.B., Egelseer E.M., Ilk N., Pum D., Schuster B. S-layers as a basic building block in a molecular construction kit. FEBS J 2007, 274:323-334.
13. Sleytr U.B., Huber C., Ilk N., Pum D., Schuster B., Egelseer E. S-Layers as a tool kit for nanobiotechnological applications. FEMS Microbiol Lett 2007, 267:131-144.
14. Huber C., Egelseer E.M., Ilk N., Sleytr U.B., Sára M. S-layer-streptavidin fusion proteins and S-layer-specific heteropolysaccharides as part of a biomolecular construction kit for application in nanobiotechnology. Microelectron Eng 2006, 83:1589-1593.
15. Huber C., Liu J., Egelseer E.M., Moll D., Knoll W., Sleytr U.B., Sára M. Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as nanoarrayed template for biochip development. Small 2006, 2:142-150.
16. Moll D., Huber C., Schlegel B., Pum D., Sleytr U.B., Sára M. S-layer-streptavidin fusion proteins as template for nanopatterned molecular arrays. Proc Natl Acad Sci U S A 2002, 99:14646-14651.
17. Pleschberger M., Saerens D., Weigert S., Sleytr U.B., Muyldermans S., Sára M., Egelseer E.M. An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology. Bioconjug Chem 2004, 15:664-671.
18. Pleschberger M., Neubauer A., Egelseer E.M., Weigert S., Lindner B., Sleytr U.B., Muyldermans S., Sára M. Generation of a functional monomolecular protein lattice consisting of an S-layer fusion protein comprising the variable domain of a camel heavy chain antibody. Bioconjug Chem 2003, 14:440-448.
19. Tschiggerl H., Casey J.L., Parisi K., Foley M., Sleytr U.B. Display of a peptide mimotope on a crystalline bacterial cell surface layer (S-layer) lattice for diagnosis of Epstein-Barr virus infection. Bioconjug Chem 2008, 19:860-865.
20. Völlenkle C., Weigert S., Ilk N., Egelseer E., Weber V., Loth F., Falkenhagen D., Sleytr U.B., Sára M. Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification. Appl Environ Microbiol 2004, 70:1514-1521. Highlight in Nature Reviews Microbiology 1512(1515), 1353.
21. Bohle B., Breitwieser A., Zwölfer B., Jahn-Schmid B., Sára M., Sleytr U.B., Ebner C. A novel approach to specific allergy treatment: the recombinant fusion protein of a bacterial cell surface (S-layer) protein and the major birch pollen allergen Bet v 1 (rSbsC-Bet v 1) combines reduced allergenicity with immunomodulating capacity. J Immunol 2004, 172:6642-6648.
22. Breitwieser A., Egelseer E.M., Moll D., Ilk N., Hotzy C., Bohle B., Ebner C., Sleytr U.B., Sára M. A recombinant bacterial cell surface (S-layer)-major birch pollen allergen-fusion protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly structured monomolecular lattices and the functionality of the allergen. Protein Eng 2002, 15:243-249.
23. Ilk N., Völlenkle C., Egelseer E.M., Breitwieser A., Sleytr U.B., Sára M. Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen. Appl Environ Microbiol 2002, 68:3251-3260.
24. Gerstmayr M., Ilk N., Jahn-Schmid B., Sleytr U.B., Bohle B. Natural self-assembly of allergen-S-layer fusion proteins is no prerequisite for reduced allergenicity and T cell stimulatory capacity. Int Arch Allergy Immunol 2009, 149:231-238.
25. Gerstmayr M., Ilk N., Schabussova I., Jahn-Schmid B., Egelseer E.M., Sleytr U.B., Ebner C., Bohle B. A novel approach to specific allergy treatment: the recombinant allergen-S-layer fusion protein rSbsC-Bet v 1 matures dendritic cells that prime Th0/Th1 and IL-10-producing regulatory T cells. J Immunol 2007, 179:7270-7275.
26. Avall-Jääskeläinen S., Kylä-Nikkilä K., Kahala M., Miikkulainen-Lahti T., Palva A. Surface display of foreign epitopes on the Lactobacillus brevis S-layer. Appl Environ Microbiol 2002, 68:5943-5951.
27. Kuen B., Sleytr U.B., Lubitz W. Sequence analysis of the sbsA gene encoding the 130-kDa surface-layer protein of Bacillus stearothermophilus strain PV72. Gene 1994, 145:115-120.
28. Riedmann E.M., Kyd J.M., Smith A.M., Gomez-Gallego S., Jalava K., Cripps A.W., Lubitz W. Construction of recombinant S-layer proteins (rSbsA) and their expression in bacterial ghosts-a delivery system for the nontypeable Haemophilus influenzae antigen Omp26. FEMS Immunol Med Microbiol 2003, 37:185-192.
29. Kern J.W., Schneewind O. BslA, a pXO1-encoded adhesin of Bacillus anthracis. Mol Microbiol 2008, 68:504-515.
30. Mesnage S., Tosi-Couture E., Fouet A. Production and cell surface anchoring of functional fusions between the SLH motifs of the Bacillus anthracis S-layer proteins and the Bacillus subtilis levansucrase. Mol Microbiol 1999, 31:927-936.
31. Mesnage S., Weber-Levy M., Haustant M., Mock M., Fouet A. Cell surface-exposed tetanus toxin fragment C produced by recombinant Bacillus anthracis protects against tetanus toxin. Infect Immun 1999, 67:4847-4850.
32. Bingle W.H., Nomellini J.F., Smit J. Cell-surface display of a Pseudomonas aeruginosa strain K pilin peptide within the paracrystalline S-layer of Caulobacter crescentus. Mol Microbiol 1997, 26:277-288.
33. Umelo-Njaka E., Nomellini J.F., Bingle W.H., Glasier L.G., Irvin R.T., Smit J. Expression and testing of Pseudomonas aeruginosa vaccine candidate proteins prepared with the Caulobacter crescentus S-layer protein expression system. Vaccine 2001, 19:1406-1415.
34. Duncan G., Tarling C.A., Bingle W.H., Nomellini J.F., Yamage M., Dorocicz I.R., Withers S.G., Smit J. Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: fusion with the beta-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product. Appl Biochem Biotechnol 2005, 127:95-110.
35. Nomellini J.F., Duncan G., Dorocicz I.R., Smit J. S-layer-mediated display of the immunoglobulin G-binding domain of streptococcal protein G on the surface of Caulobacter crescentus: development of an immunoactive reagent. Appl Environ Microbiol 2007, 73:3245-3253.
36. Nomellini J.F., Li C., Lavallee D., Shanina I., Cavacini L.A., Horwitz M.S., Smit J. Development of an HIV-1 specific microbicide using Caulobacter crescentus S-layer mediated display of CD4 and MIP1alpha. PLoS One 2010, 5:e10366.
37. Simon B., Nomellini J.F., Chiou P., Binale W., Thornton J., Smit J., Leong J.A. Recombinant vaccines against infectious hematopoietic necrosis virus: production line by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials. Dis Aquat Org 2001, 44:400-402.
38. Bhatnagar P.K., Awasthi A., Nomellini J.F., Smit J., Suresh M.R. Anti-tumor effects of the bacterium Caulobacter crescentus in murine tumor models. Cancer Biol Ther 2006, 5:485-491.
39. Georgiou G., Stathopoulos C., Daugherty P.S., Nayak A.R., Iverson B.L., Curtiss R. Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. Nat Biotechnol 1997, 15:29-34.
40. Nomellini J.F., Toporowski M.C., Smit J. Secretion or presentation of recombinant proteins and peptides mediated by the S-layer of Caulobacter crescentus. Expression Technologies: Current Status and Future Trends 2004, 477-524. Horizon Scientific Press. F. Banex (Ed.).
41. Poobalane S., Thompson K.D., Ardo L., Verjan N., Han H.J., Jeney G., Hirono I., Aoki T., Adams A. Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish. Vaccine 2010, 28:3540-3547.
42. Tschiggerl H., Breitwieser A., de Roo G., Verwoerd T., Schäffer C., Sleytr U.B. Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus. J Biotechnol 2008, 133:403-411.
43. Schäffer C., Novotny R., Küpcü S., Zayni S., Scheberl A., Friedmann J., Sleytr U.B., Messner P. Novel biocatalysts based on S-layer self-assembly of Geobacillus stearothermophilus NRS 2004/3a: a nanobiotechnological approach. Small 2007, 3:1549-1559.
44. Kainz B., Steiner K., Moller M., Pum D., Schäffer C., Sleytr U.B., Toca-Herrera J.L. Absorption, steady-state fluorescence, fluorescence lifetime, and 2D self-assembly properties of engineered fluorescent S-layer fusion proteins of Geobacillus stearothermophilus NRS 2004/3a. Biomacromolecules 2010, 11:207-214.
45. Kainz B., Steiner K., Sleytr U.B., Pum D., Toca-Herrera J.L. Fluorescence energy transfer in the bi-fluorescent S-layer tandem fusion protein ECFP-SgsE-YFP. J Struct Biol 2010, 172:276-283.
46. Ilk N., Küpcü S., Moncayo G., Klimt S., Ecker R.C., Hofer-Warbinek R., Egelseer E.M., Sleytr U.B., Sára M. A functional chimaeric S-layer-enhanced green fluorescent protein to follow the uptake of S-layer-coated liposomes into eukaryotic cells. Biochem J 2004, 379:441-448.
47. Badelt-Lichtblau H., Kainz B., Völlenkle C., Egelseer E.M., Sleytr U.B., Pum D., Ilk N. Genetic engineering of the S-layer protein SbpA of Lysinibacillus sphaericus CCM 2177 for the generation of functionalized nanoarrays. Bioconjug Chem 2009, 20:895-903.
48. Pollmann K., Raff J., Merroun M., Fahmy K., Selenska-Pobell S. Metal binding by bacteria from uranium mining waste piles and its technological applications. Biotechnol Adv 2006, 24:58-68.
49. Velasquez L., Dussan J. Biosorption and bioaccumulation of heavy metals on dead and living biomass of Bacillus sphaericus. J Hazard Mater 2009, 167:713-716.
50. Patel J., Zhang Q., McKay R.M., Vincent R., Xu Z. Genetic engineering of Caulobacter crescentus for removal of cadmium from water. Appl Biochem Biotechnol 2010, 160:232-243.
51. Ilk N., Egelseer E.M., Ferner-Ortner J., Küpcü S., Pum D., Schuster B., Sleytr U.B. Surfaces functionalized with self-assembling S-layer fusion proteins for nanobiotechnological applications. Colloids Surf A: Physicochem Eng Aspects 2008, 321:163-167.
52. Pum D, Sleytr UB: Protein-based nanobioelectronics. In Nanobioelectronics for Electronics, Biology, and Medicine Edited by Offenhäusser A, Rinaldi R: Springer; 2009:167-180. [Lockwood D (Series Editor): Nanostructure Science and Technology.].
53. Schuster B., Sleytr U.B. Composite S-layer lipid structures. J Struct Biol 2009, 168:207-216.