Construction of recombinant S-layer proteins (rSbsA) and their expression in bacterial ghosts - A delivery system for the nontypeable Haemophilus influenzae antigen Omp26

FEMS Immunology and Medical Microbiology

Volumn 37, Issue 2-3, 2003, Pages 185-192

  Riedmann, Eva M ^a,b   Kyd, Jennelle M ^c   Smith, Adam M ^c   Gomez Gallego, Sara ^c   Jalava, Katri ^b   Cripps, Allan W ^c   Lubitz, Werner ^a,b  

^a UNIVERSITY OF VIENNA   (Austria)

^b Bird C GmbH   (Austria)

^c UNIVERSITY OF CANBERRA   (Australia)

Author keywords

Bacterial ghost; Candidate vaccine; Nontypeable Haemophilus influenzae; Recombinant surface layer

Indexed keywords

ANTIBODY; ANTISERUM; BACTERIAL ANTIGEN; BACTERIAL VACCINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; OMP 26 ANTIGEN; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN 26; RECOMBINANT PROTEIN; SURFACE LAYER PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY RESPONSE; ARTICLE; BACILLUS; BACILLUS STEAROTHERMOPHILLUS; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL INFECTION; BACTERIAL MEMBRANE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DRUG ADMINISTRATION ROUTE; DRUG DELIVERY SYSTEM; DRUG EFFECT; ELECTRON MICROSCOPY; ESCHERICHIA COLI; EXPERIMENT; FEASIBILITY STUDY; HAEMOPHILUS INFLUENZAE; IMMUNOGENICITY; LYSIS; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN STRUCTURE; WESTERN BLOTTING;

EID: 0038433313     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(03)00070-1     Document Type: Article

References (41)

1. Witte A., Bläsi U., Halfmann G., Szostak M., Wanner G., Lubitz W. Phi X174 protein E-mediated lysis of Escherichia coli. Biochimie. 72:1990;191-200.
2. Witte A., Wanner G., Bläsi U., Halfmann G., Szostak M., Lubitz W. Endogenous transmembrane tunnel formation mediated by phi X174 lysis protein E. J. Bacteriol. 172:1990;4109-4114.
3. Witte A., Wanner G., Sulzner M., Lubitz W. Dynamics of PhiX174 protein E-mediated lysis of Escherichia coli. Arch. Microbiol. 157:1992;381-388.
4. Eko F.O., Hensel A., Bunka S., Lubitz W. Immunogenicity of Vibrio cholerae ghosts following intraperitoneal immunization of mice. Vaccine. 12:1994;1330-1334.
5. Huter V., Hensel A., Brand E., Lubitz W. Improved protection against lung colonization by Actinobacillus pleuropneumoniae ghosts: characterization of a genetically inactivated vaccine. J. Biotech. 83:2000;161-172.
6. Hensel A., Huter V., Katinger A., Raza P., Strnistschie C., Roesler U., Brand E., Lubitz W. Intramuscular immunization with genetically inactivated (ghosts) Actinobacillus pleuropneumoniae serotype 9 protects pigs against homologous aerosol challenge and prevents carrier state. Vaccine. 18:2000;2945-2955.
7. Lubitz W. Bacterial ghosts as carrier and targeting systems. Expert Opin. Biol. Ther. 1:2001;1-7.
8. Sleytr U.B., Beveridge T.J. Bacterial S-layers. Trends Microbiol. 7:1999;253-260.
9. Sára M., Sleytr U.B. S-layer proteins. J. Bacteriol. 182:2000;859-868.
10. Sára M., Kuen B., Mayer H.F., Mandl F., Schuster K.C., Sleytr U.B. Dynamics in oxygen-induced changes in S-layer protein synthesis from Bacillus stearothermophilus PV72 and the S-layer-deficient variant T5 in continuous culture and studies of the cell wall composition. J. Bacteriol. 178:1996;2108-2117.
11. Kuen B., Koch A., Asenbauer E., Sára M., Lubitz W. Molecular characterization of the Bacillus stearothermophilus PV72 S-layer gene sbsB induced by oxidative stress. J. Bacteriol. 179:1997;1664-1670.
12. Scholz H., Riedmann E., Witte A., Lubitz W., Kuen B. S-layer variation in Bacillus stearothermophilus PV72 is based on DNA rearrangements between the chromosome and the naturally occurring megaplasmids. J. Bacteriol. 183:2001;1672-1679.
13. Kuen B., Sleytr U.B., Lubitz W. Sequence analysis of the sbsA gene encoding the 130-kDa surface-layer protein of Bacillus stearothermophilus strain PV72. Gene. 145:1994;115-120.
14. Kuen B., Sára M., Lubitz W. Heterologous expression and self-assembly of the S-layer protein SbsA of Bacillus stearothermophilus in Escherichia coli. Mol. Microbiol. 19:1995;495-503.
15. Truppe M., Howorka S., Schroll G., Lechleitner S., Kuen B., Resch S., Lubitz W. Biotechnological applications of recombinant S-layer proteins rSbsA and rSbsB from Bacillus stearothermophilus PV72. FEMS Microbiol. Rev. 20:1997;88-91.
16. Breitwieser A., Egelseer E.M., Moll D., Ilk N., Hotzy C., Bohle B., Ebner C., Sleytr U.B., Sára M. A recombinant bacterial cell surface (S-layer)-major birch pollen allergen-fusion protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly structured monomolecular lattices and the functionality of the allergen. Protein Eng. 15:2002;243-249.
17. Ilk N., Völlenkle C., Egelseer E.M., Breitwieser A., Sleytr U.B., Sára M. Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused antigen. Appl. Environ. Microbiol. 68:2002;3251-3260.
18. Foxwell R.A., Kyd J.M., Cripps A.W. Nontypeable Haemophilus influenzae: Pathogenesis and prevention. Microbiol. Mol. Biol. Rev. 62:1998;294-308.
19. St Geme J.W. III. The pathogenesis of nontypeable Haemophilus influenzae otitis media. Vaccine. 19:2001;41-50.
20. Cripps A.W., Taylor D., Wallace F.J., Clancy R.L. Respiratory immunity stimulated by intestinal immunization with purified nontypeable Haemophilus influenzae antigens. J. Infect. Dis. 165:(Suppl. 1):1992;199-201.
21. Murphy T.F., Nelson M.B., Apicella M.A. The P6 outer membrane protein of nontypeable Haemophilus influenzae as a vaccine antigen. J. Infect. Dis. 165:(Suppl. 1):1992;203-205.
22. Green B.A., Vazquez M.E., Zlotnick G.W., Quigley-Reape G., Swarts J.D., Green I., Cowell J.L., Bluestone C.D., Doyle W.J. Evaluation of mixtures of purified Haemophilus influenzae outer membrane proteins in protection against challenge with nontypeable H. influenzae in the chinchilla otitis media model. Infect. Immun. 61:1993;1950-1957.
23. Kyd J.M., Dunkley M.L., Cripps A.W. Enhanced respiratory clearance of nontypeable Haemophilus influenzae following mucosal immunization with P6 in a rat model. Infect. Immun. 63:1995;2931-2940.
24. Kyd J.M., Cripps A.W. Modulation of antigen-specific T and B cell responses influence bacterial clearance of nontypeable Haemophilus influenzae from the lung in a rat model. Vaccine. 14:1996;1471-1478.
25. Bolduc G.R., Bouchet V., Jiang R.Z., Geisselsoder J., Truong-Bolduc Q.C., Rice P.A., Pelton S.I., Goldstein R. Variability of outer membrane protein P1 and its evaluation as a vaccine candidate against experimental otitis media due to nontypeable Haemophilus influenzae: an unambiguous, multifaceted approach. Infect. Immun. 68:2000;4505-4517.
26. Kyd J.M., Cripps A.W. Potential of a novel protein, OMP26, from nontypeable Haemophilus influenzae to enhance pulmonary clearance in a rat model. Infect. Immun. 66:1998;2272-2278.
27. El-Adhami W., Kyd J.M., Bastin D.A., Cripps A.W. Characterization of the gene encoding a 26-kilodalton protein (OMP26) from nontypeable Haemophilus influenzae and the immune responses to the recombinant protein. Infect. Immun. 67:1999;1935-1942.
28. Poolman J.T., Bakaletz L., Cripps A., Denoel P.A., Forsgren A., Kyd J., Lobet Y. Developing a nontypeable Haemophilus influenzae (NTHi) vaccine. Vaccine. 19:2001;108-115.
29. Lubitz, W. and Resch, S. (1998) Secretion of carrier-bound proteins into the periplasm and into the extracellular space. Patent: International publication number PCT/EP98/04723.
30. Szostak M.P., Hensel A., Eko F.O., Klein R., Auer T., Mader H., Haslberger A., Bunka S., Wanner G., Lubitz W. Bacterial ghosts: non-living candidate vaccines. J. Biotech. 44:1996;161-170.
31. Kloos D.U., Stratz M., Guttler A., Steffan R.J., Timmis K.N. Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death. J. Bacteriol. 176:1994;7352-7361.
32. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
33. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. BioTechniques. 24:1992;145-149.
34. Messner P., Mazid M.A., Unger F.M., Sleytr U.B. Artificial antigens. Synthetic carbohydrate haptens immobilized on crystalline bacterial surface layer glycoproteins. Carbohydr. Res. 233:1992;175-184.
35. Smith R.H., Messner P., Lamontage L.R., Sleytr U.B., Unger F.M. Induction of T-cell immunity to oligosaccharide antigens immobilized on crystalline bacterial surface layers (S-layers). Vaccine. 11:1993;919-924.
36. H1-controlled vaccination responses: vaccine applications of bacterial surface layer proteins. J. Biotech. 44:1996;225-231.
37. Egelseer E.M., Leitner K., Jarosch M., Hotzy C., Zayni S., Sleytr U.B., Sára M. The S-layer proteins of two Bacillus stearothermophilus wild-type strains are bound via their N-terminal region to a secondary cell wall polymer of identical chemical composition. J. Bacteriol. 180:1998;1488-1495.
38. Howorka S., Sára M., Wang Y., Kuen B., Sleytr U.B., Lubitz W., Bayley H. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. J. Biol. Chem. 275:2000;37876-37886.
39. Jarosch M., Egelseer E.M., Mattanovich D., Sleytr U.B., Sára M. S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli. Microbiology. 146:2000;273-281.
40. Messner, P., Unger, F.M. and Sleytr, U.B. (1996) Vaccine development based on S-layer technology. In: Crystalline Bacterial Cell Surface Proteins (Sleytr, U.B., Messner, P., Pum, D. and Sára, M., Eds.), pp. 161-173. R.G. Landes Comp., Austin, TX; Academic Press, San Diego, CA.
41. Kyd J., Cripps A. Identifying vaccine antigens and assessing delivery systems for the prevention of bacterial infections. J. Biotech. 83:2000;85-90.